Lessons from an α-Helical Membrane Enzyme: Expression, Purification, and Detergent Optimization for Biophysical and Structural Characterization

2016 ◽  
pp. 281-301
Author(s):  
Jennifer L. Johnson ◽  
Sibel Kalyoncu ◽  
Raquel L. Lieberman
Keyword(s):  
Structural Characterization ◽  
Enzyme Expression ◽  
Membrane Enzyme

Phase behavior of cationic and anionic surfactants at low concentrations

1992 ◽  
Vol 50 (1) ◽  
pp. 694-695
Author(s):  
E. Naranjo
Keyword(s):  
Phase Behavior ◽  
Structural Characterization ◽  
Dynamic Systems ◽  
Anionic Surfactants ◽  
Intermolecular Forces ◽  
Stable Form ◽  
Surfactant Mixtures ◽  
Low Concentrations ◽  
Cationic And Anionic Surfactants ◽  
General Method

Equilibrium vesicles, those which are the stable form of aggregation and form spontaneously on mixing surfactant with water, have never been demonstrated in single component bilayers and only rarely in lipid or surfactant mixtures. Designing a simple and general method for producing spontaneous and stable vesicles depends on a better understanding of the thermodynamics of aggregation, the interplay of intermolecular forces in surfactants, and an efficient way of doing structural characterization in dynamic systems.

New ultrastructural findings about Borrelia burgdorferi by cryofixation, negative staining, thin sectioning and scanning techniques

1990 ◽  
Vol 48 (3) ◽  
pp. 582-583
Author(s):  
S. F. Hayes ◽  
M. D. Corwin ◽  
T. G. Schwan ◽  
D. W. Dorward ◽  
W. Burgdorfer
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Structural Characterization ◽  
Negative Staining ◽  
Low Speed ◽  
Thin Sectioning ◽  
Ultrastructural Findings

Characterization of Borrelia burgdorferi strains by means of negative staining EM has become an integral part of many studies related to the biology of the Lyme disease organism. However, relying solely upon negative staining to compare new isolates with prototype B31 or other borreliae is often unsatisfactory. To obtain more satisfactory results, we have relied upon a correlative approach encompassing a variety EM techniques, i.e., scanning for topographical features and cryotomy, negative staining and thin sectioning to provide a more complete structural characterization of B. burgdorferi.For characterization, isolates of B. burgdorferi were cultured in BSK II media from which they were removed by low speed centrifugation. The sedimented borrelia were carefully resuspended in stabilizing buffer so as to preserve their features for scanning and negative staining. Alternatively, others were prepared for conventional thin sectioning and for cryotomy using modified procedures. For thin sectioning, the fixative described by Ito, et al.

STRUCTURAL CHARACTERIZATION, PREPARATION AND MAGNETIC PROPERTIES OF SEVERAL ROOM-TEMPERATURE MAGNETIC OXIDES WITH THE PEROVSKITE-LIKE STRUCTURE

1977 ◽  
Vol 38 (C1) ◽  
pp. C1-95-C1-101 ◽  
Author(s):  
B. BOCHU ◽  
M. N. DESCHIZEAUX ◽  
J. C. JOUBERT ◽  
J. CHENAVAS ◽  
A. COLLOMB ◽  
...  
Keyword(s):  
Magnetic Properties ◽  
Structural Characterization ◽  
Room Temperature ◽  
Magnetic Oxides

Structural characterization of the PPIase domain of FKBP51, a cochaperone of human Hsp90

2011 ◽  
Vol 44 (06) ◽  
Author(s):  
A Bracher ◽  
C Kozany ◽  
AK Thost ◽  
F Hausch
Keyword(s):  
Structural Characterization ◽  
Ppiase Domain

Structural Characterization and Bioactivity of Cranberry Oligosaccharides

Planta Medica ◽  
2013 ◽  
Vol 79 (10) ◽  
Author(s):  
KM Auker ◽  
CM Coleman ◽  
M Wang ◽  
B Avula ◽  
L Kimble ◽  
...  
Keyword(s):  
Structural Characterization

Structural characterization of dendrobine-type sesquiterpene alkaloids from the stems of Dendrobium nobile using LC-MS/MS

Planta Medica ◽  
2014 ◽  
Vol 80 (10) ◽  
Author(s):  
YH Wang ◽  
B Avula ◽  
N Abe ◽  
F Wei ◽  
M Wang ◽  
...  
Keyword(s):  
Structural Characterization ◽  
Dendrobium Nobile
Sign in / Sign up

Export Citation Format

Share Document