Characterization of Inhibitor Binding Through Multiple Inhibitor Analysis: A Novel Local Fitting Method

2016 ◽  
pp. 33-45 ◽  
Author(s):  
Thomas V. Riera ◽  
Tim J. Wigle ◽  
Robert A. Copeland
Keyword(s):  
Inhibitor Binding ◽  
Fitting Method ◽  
Inhibitor Analysis ◽  
Local Fitting

Characterization of inhibitor binding to human kinesin spindle protein by site-directed mutagenesis

2009 ◽  
Vol 484 (1) ◽  
pp. 1-7 ◽  
Author(s):  
C. Gary Marshall ◽  
Maricel Torrent ◽  
Olusegun Williams ◽  
Kelly A. Hamilton ◽  
Carolyn A. Buser
Keyword(s):  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Inhibitor Binding ◽  
Kinesin Spindle Protein

scholarly journals Characterization of the Vortex Beam by Fermat’s Spiral

Photonics ◽  
2020 ◽  
Vol 7 (4) ◽  
pp. 102
Author(s):  
Ewa Frączek ◽  
Agnieszka Popiołek-Masajada ◽  
Sławomir Szczepaniak
Keyword(s):  
Phase Distribution ◽  
Phase Plate ◽  
Vortex Beam ◽  
Scanning Microscope ◽  
Object Beam ◽  
Fitting Method ◽  
Numerical Fitting ◽  
Helical Beam ◽  
Spiral Phase

In this paper, we characterize the helical beam structure through an analysis of the spiral character of the phase distribution inside a light beam. In particular, we show that a line connected with the 2π phase jump in the Laguerre–Gauss beam can be described by a Fermat’s spiral. We propose a numerical fitting method to determine the parameters of a spiral equation for the phase distribution of the helical beam. Next, we extend the procedure to a vortex beam created by the spiral phase plate and apply it to experimental phase maps, which allows us to recover the phase shift introduced into the object beam in the optical vortex scanning microscope.

scholarly journals Identification of three critical acidic residues of poly(ADP-ribose) glycohydrolase involved in catalysis: determining the PARG catalytic domain

2005 ◽  
Vol 388 (2) ◽  
pp. 493-500 ◽  
Author(s):  
Chandra N. PATEL ◽  
David W. KOH ◽  
Myron K. JACOBSON ◽  
Marcos A. OLIVEIRA
Keyword(s):  
Catalytic Activity ◽  
Catalytic Domain ◽  
Functional Characterization ◽  
Sequence Alignments ◽  
Inhibitor Binding ◽  
Conserved Sequence ◽  
Binding Residue ◽  
Acidic Residues ◽  
Hydrolysis Of

PARG [poly(ADP-ribose) glycohydrolase] catalyses the hydrolysis of α(1″→2′) or α(1‴→2″) O-glycosidic linkages of ADP-ribose polymers to produce free ADP-ribose. We investigated possible mechanistic similarities between PARG and glycosidases, which also cleave O-glycosidic linkages. Glycosidases typically utilize two acidic residues for catalysis, thus we targeted acidic residues within a conserved region of bovine PARG that has been shown to contain an inhibitor-binding site. The targeted glutamate and aspartate residues were changed to asparagine in order to minimize structural alterations. Mutants were purified and assayed for catalytic activity, as well as binding, to an immobilized PARG inhibitor to determine ability to recognize substrate. Our investigation revealed residues essential for PARG catalytic activity. Two adjacent glutamic acid residues are found in the conserved sequence Gln755-Glu-Glu757, and a third residue found in the conserved sequence Val737-Asp-Phe-Ala-Asn741. Our functional characterization of PARG residues, along with recent identification of an inhibitor-binding residue Tyr796 and a glycine-rich region Gly745-Gly-Gly747 important for PARG function, allowed us to define a PARG ‘signature sequence’ [vDFA-X3-GGg-X6–8-vQEEIRF-X3-PE-X14-E-X12-YTGYa], which we used to identify putative PARG sequences across a range of organisms. Sequence alignments, along with our mapping of PARG functional residues, suggest the presence of a conserved catalytic domain of approx. 185 residues which spans residues 610–795 in bovine PARG.

scholarly journals A characterization of dominant local Fitting classes

2012 ◽  
Vol 358 ◽  
pp. 27-32 ◽  
Author(s):  
P. Hauck ◽  
V.N. Zahursky
Keyword(s):  
Fitting Classes ◽  
Local Fitting

Characterization and Formulation of Microstrip-Slot Impedance with Different Thickness and Relative Permittivity

2015 ◽  
Vol 781 ◽  
pp. 53-56
Author(s):  
Khairul Huda Yusof ◽  
Norhudah Seman ◽  
Mohd Haizal Jamaluddin ◽  
D.N.A. Zaidel
Keyword(s):  
Curve Fitting ◽  
Relative Permittivity ◽  
Slot Line ◽  
Fitting Method ◽  
Curve Fitting Method ◽  
Different Thickness ◽  
Cst Microwave Studio

This paper presents the characterization of a microstrip-slot line in terms of its impedance and dimension. The characterization is made by referring to the analysis of different relative permittivity and thicknesses of substrates, which conducted by using CST Microwave Studio. Based on the characterization with the use of completing square curve fitting method, new formulated equations are proposed for microstrip-slot line. These formulated equations are suitable for thin substrate with low relative permittivity. The characterization is made based on the substrates with the selected thickness of 0.508 mm, 0.762 mm and 1.542 mm, for relative permittivity of 4.5 (TMM4), 3.38 (RO 4003C) and 2.94 (RT 6002).

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