Evaluation of Molecular Interaction between CCN2 Protein and Its Binding Partners by Surface Plasmon Resonance (SPR)

2016 ◽  
pp. 169-176 ◽  
Author(s):  
Eriko Aoyama ◽  
Masaharu Takigawa
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Molecular Interaction ◽  
Binding Partners ◽  
Ccn2 Protein

scholarly journals Surface functionalization of SPR chip for specific molecular interaction analysis under flow condition

2017 ◽  
Vol 10 (02) ◽  
pp. 1650040 ◽  
Author(s):  
Tao Ma ◽  
Xiaoxia Chen ◽  
Qing Peng ◽  
Pengfei Zhang ◽  
Yonghong He
Keyword(s):  
Aqueous Solution ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Surface Functionalization ◽  
Plasmon Resonance ◽  
Molecular Interaction ◽  
Flow Condition ◽  
Interaction Analysis ◽  
Low Cost ◽  
Protein A

Surface functionalization of sensor chip for probe immobilization is crucial for the biosensing applications of surface plasmon resonance (SPR) sensors. In this paper, we report a method circulating the dopamine aqueous solution to coat polydopamine film on sensing surface for surface functionalization of SPR chip. The polydopamine film with available thickness can be easily prepared by controlling the circulation time and the biorecognition elements can be immobilized on the polydopamine film for specific molecular interaction analysis. These operations are all performed under flow condition in the fluidic system, and have the advantages of easy implementation, less time consuming, and low cost, because the reagents and devices used in the operations are routinely applied in most laboratories. In this study, the specific absorption between the protein A probe immobilized on the sensing surface and human immunoglobulin G in the buffer is monitored based on this surface functionalization strategy to demonstrated its feasibility for SPR biosensing applications.

scholarly journals MDM2–MDM4 molecular interaction investigated by atomic force spectroscopy and surface plasmon resonance

2016 ◽  
Vol Volume 11 ◽  
pp. 4221-4229 ◽  
Author(s):  
Salvatore Cannistraro ◽  
Ilaria Moscetti ◽  
Emanuela Teveroni ◽  
Fabiola Moretti ◽  
Anna Rita Bizzarri
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Molecular Interaction ◽  
Force Spectroscopy ◽  
Atomic Force Spectroscopy ◽  
Atomic Force

Comparison of sensitivity of the refractometric methods of frustrated total internal reflection and surface plasmon resonance

2020 ◽  
pp. 44-49
Author(s):  
I. N. Pavlov
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Total Internal Reflection ◽  
Resonance Method ◽  
Internal Reflection ◽  
Optical Methods ◽  
Thin Boundary Layer ◽  
Experimental Conditions ◽  
Frustrated Total Internal Reflection

Two optical methods, namely surface plasmon resonance imaging and frustrated total internal reflection, are described in the paper in terms of comparing their sensitivity to change of refractive index of a thin boundary layer of an investigated medium. It is shown that, despite the fact that the theoretically calculated sensitivity is higher for the frustrated total internal reflection method, and the fact that usually in practice the surface plasmon resonance method, on the contrary, is considered more sensitive, under the same experimental conditions both methods show a similar result.

Surface Plasmon Resonance Based Sensitive Immunosensor for Benzaldehyde Detection

2010 ◽  
Vol 130 (7) ◽  
pp. 269-274 ◽  
Author(s):  
Takeshi Onodera ◽  
Takuzo Shimizu ◽  
Norio Miura ◽  
Kiyoshi Matsumoto ◽  
Kiyoshi Toko
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance
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