Chemiluminescence-Based Detection of Peptide Activity and Peptide-Receptor Binding in Plants

2017 ◽  
pp. 287-295
Author(s):  
Mari Wildhagen ◽  
Markus Albert ◽  
Melinka A. Butenko
Keyword(s):  
Receptor Binding ◽  
Peptide Receptor

Phospholipids stabilize binding of pituitary adenylate cyclase-activating peptide to vasoactive intestinal polypeptide receptor

2021 ◽  
Author(s):  
Nidhin Thomas ◽  
Ashutosh Agrawal
Keyword(s):  
Adenylate Cyclase ◽  
Receptor Binding ◽  
Vasoactive Intestinal Polypeptide ◽  
Binding Free Energy ◽  
Active Role ◽  
Lipid Binding ◽  
Peripheral Tissues ◽  
Peptide Receptor ◽  
Pituitary Adenylate Cyclase ◽  
Intestinal Polypeptide

Vasoactive intestinal polypeptide receptor (VIP1R) is a class B G-protein coupled receptor (GPCR) that is widely distributed throughout the central nervous system, T-lymphocytes, and peripheral tissues of organs like lungs and liver. Critical functions of these receptors render them potential pharmacological targets for the treatment of a broad spectrum of inflammatory and neurodegenerative diseases. Here we use atomistic studies to show that phospholipids can act as potent regulators of peptide binding on to the receptor. We simulated the binding of neuropeptide pituitary adenylate cyclase-activating peptide (PACAP27) into the transmembrane bundle of the receptor. The simulations reveal two lipid binding sites on the peptidic ligand for the negatively charged phosphodiester of phospholipids in the extracellular leaflet which lower the peptide-receptor binding free energy by ~8kBT. We further simulated the effect of anionic lipids phosphatidylinositol-4,5-bisphosphate (PIP2). These lipids show much stronger interaction, lowering the peptide-receptor binding energy by an additional ~7kBT compared to POPC lipids. These findings suggest that lipids can play an active role in catalyzing peptide-receptor binding and activating vasoactive intestinal polypeptide receptors.

Development of peptide receptor binding assays: Methods to avoid false negatives

2009 ◽  
Vol 158 (1-3) ◽  
pp. 97-102 ◽  
Author(s):  
Valentijn Vergote ◽  
Sylvia Van Dorpe ◽  
Mathieu Verbeken ◽  
Christian Burvenich ◽  
Christophe Van de Wiele ◽  
...  
Keyword(s):  
Receptor Binding ◽  
Binding Assays ◽  
False Negatives ◽  
Peptide Receptor

Application of the multipin peptide synthesis technique for peptide receptor binding studies: Substance P as A model system

1993 ◽  
Vol 3 (3) ◽  
pp. 447-450 ◽  
Author(s):  
Jian-xin. Wang ◽  
Andrew M. Bray ◽  
Angela J. DiPasquale ◽  
N.Joe Maeji ◽  
H.Mario Geysen
Keyword(s):  
Substance P ◽  
Peptide Synthesis ◽  
Receptor Binding ◽  
Model System ◽  
Binding Studies ◽  
Peptide Receptor ◽  
Synthesis Technique

Effects of ibuprofen on chemotactic peptide-receptor binding and granulocyte response

1986 ◽  
Vol 35 (19) ◽  
pp. 3349-3354 ◽  
Author(s):  
Keith M. Skubixz ◽  
Dale E. Hammerschmidt
Keyword(s):  
Receptor Binding ◽  
Chemotactic Peptide ◽  
Peptide Receptor

Changes in Parathyroid Hormone-Related Peptide Receptor Binding in the Shell Gland of Laying Hens (Gallus domesticus) during the Oviposition Cycle

2000 ◽  
Vol 117 (2) ◽  
pp. 182-188 ◽  
Author(s):  
Teruko Ieda ◽  
Tetsuya Takahashi ◽  
Noboru Saito ◽  
Tadashi Yasuoka ◽  
Mitsuo Kawashima ◽  
...  
Keyword(s):  
Parathyroid Hormone ◽  
Receptor Binding ◽  
Laying Hens ◽  
Gallus Domesticus ◽  
Shell Gland ◽  
Peptide Receptor ◽  
Related Peptide ◽  
Parathyroid Hormone Related Peptide
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