Cell Enumeration Assays: Application of the MTT and Sulforhodamine B Assays to Lipopolysaccharide-Stimulated Neonatal Rodent Microglia

Inhibitory synaptic transmission via GABA and glycine receptors plays a crucial role in shaping the excitatory response of neurons in the retina. Whole-cell recordings were obtained from ganglion cells in the intact rabbit eyecup preparation to correlate GABA- and glycine-activated currents with the presence of their specific receptors on morphologically identified α ganglion cells. Alpha ganglion cells were chosen based upon their large somata when viewing the retinal surface, and responses to light and dark spots were used to identify OFF-alpha ganglion cells. Light responses were abolished by superfusion of Ringer's containing cobalt to synaptically isolate the cell by blocking all Ca2+-mediated transmitter release. Pressure pulses of GABA and glycine were delivered to an area that encompassed the dendritic field while receptor antagonists were applied through superfusion to characterize the direct inhibition onto the ganglion cell. Physiological results indicated that OFF-α cells did not have any GABAC receptor-activated currents, but did express currents mediated by ionotropic GABAA receptors and metabotropic GABAB receptors that were blocked by their specific antagonists bicuculline and CGP55845, respectively. The amplitudes of strychnine-sensitive glycine-activated currents were always larger than the currents elicited by GABA. Confocal optical sections of physiologically identified, sulforhodamine B-stained cells displayed the localization of glycine and GABAA receptor subunit labeling dispersed over the stained dendrites.

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Abstract 596: Single circulating tumor cell enumeration and targeted sequencing in non-small cell lung cancer

10.1158/1538-7445.am2021-596 ◽

2021 ◽

Author(s):

Mouadh Barbirou ◽

Yariswamy Manjunath ◽

Amanda Miller ◽

Arturo Ramirez ◽

Nolan Ericson ◽

...

Keyword(s):

Lung Cancer ◽

Tumor Cell ◽

Small Cell Lung Cancer ◽

Cell Lung Cancer ◽

Circulating Tumor Cell ◽

Small Cell ◽

Targeted Sequencing ◽

Small Cell Lung ◽

Cell Enumeration

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ZnO:Ag nanorods as efficient photocatalysts: Sunlight driven photocatalytic degradation of sulforhodamine B

Applied Surface Science ◽

10.1016/j.apsusc.2017.09.050 ◽

2018 ◽

Vol 427 ◽

pp. 863-875 ◽

Cited By ~ 34

Author(s):

Raji R. ◽

Sibi K.S. ◽

Gopchandran K.G.

Keyword(s):

Photocatalytic Degradation ◽

Sulforhodamine B

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Anticancer, antimicrobial and antioxidant potential of sterically tuned bis-N-heterocyclic salts

Zeitschrift für Naturforschung C ◽

10.1515/znc-2018-0095 ◽

2018 ◽

Vol 74 (1-2) ◽

pp. 17-23 ◽

Cited By ~ 2

Author(s):

Noor ul Huda ◽

Shamsul Islam ◽

Muhammad Zia ◽

Kainaat William ◽

Fakhar i Abbas ◽

...

Keyword(s):

Breast Adenocarcinoma ◽

Benzimidazole Derivatives ◽

Alkyl Aryl ◽

Cytotoxic Compound ◽

Sulforhodamine B ◽

Hct 116 ◽

Mrsa Strains ◽

Pharmacological Potential ◽

Derivatives Of ◽

Lipophilic Character

AbstractThe current study was conducted to evaluate the antimicrobial, antioxidant, antileishmanial and cytotoxic potential of designed derivatives of 1,1′-(1,3-phenylenebis(methylene))bis(3-alkyl/aryl-1H-benzimidazol-3-ium) salts. The antibacterial potential of the test compounds was investigated againstStaphylococcus aureus, Pseudomonas aeruginosaand two methicillin-resistantS. aureus(MRSA) strains (MRSA10, MRSA11), where compound6showed the best results. For brine shrimp lethality bioassay (BSLB), compound6again showed up to 100% mortality at 200 μg/mL and 56.7% mortality at 6.25 μg/mL. Antileishmanial assay was performed againstLeishmania tropicaat 20 μg/mL dosage, where6showed the most promising activity with 16.26% survival (83.74% mortality; IC50=14.63 μg/mL). The anticancer potential of the selected benzimidazole derivatives was evaluated against two selected cell lines (human colorectal cancer, HCT-116 and breast adenocarcinoma, MCF-7) using sulforhodamine B (SRB) assay. Compound6was found to be the most effective cytotoxic compound with 75% inhibition of HCT-116 proliferation at 1 mg/mL concentration. Succinctly,6exhibited impressive pharmacological potential that might be attributed to its higher lipophilic character owing to the longer N-substituted alkyl chains when compared to the other test compounds.

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Comparison of Different 3D Reconstruction and Classification Algorithms based on the Extended Cell Enumeration Representation

Computer Assisted Radiology / Computergestützte Radiologie ◽

10.1007/978-3-662-00807-2_186 ◽

1991 ◽

pp. 873-873

Author(s):

U. Tronnier ◽

F. Hasenbrink ◽

A. Wittich

Keyword(s):

3D Reconstruction ◽

Classification Algorithms ◽

Cell Enumeration

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Sulforhodamine B and exogenous surfactant effects on alveolar surface tension under acute respiratory distress syndrome conditions

Journal of Applied Physiology ◽

10.1152/japplphysiol.00422.2020 ◽

2020 ◽

Vol 129 (6) ◽

pp. 1505-1513 ◽

Cited By ~ 1

Author(s):

Tam L. Nguyen ◽

Carrie E. Perlman

Keyword(s):

Surface Tension ◽

Acute Respiratory Distress Syndrome ◽

Respiratory Distress Syndrome ◽

Respiratory Distress ◽

Distress Syndrome ◽

Exogenous Surfactant ◽

Sulforhodamine B ◽

Isolated Lungs ◽

Surfactant Effects ◽

Alveolar Surface

In the acute respiratory distress syndrome (ARDS), lowering surface tension, T, should reduce ventilation injury, yet exogenous surfactant has not reduced mortality. We show with direct T determination in isolated lungs that substances suggested to elevate T in ARDS indeed raise T, and exogenous surfactant reduces T. Further, we extend our previous finding that sulforhodamine B (SRB) reduces T below normal in healthy lungs and show that SRB, too, reduces T under ARDS conditions.

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