Generation of Hepatic Tissue Structures Using Multicellular Spheroid Culture

2018 ◽  
pp. 157-165 ◽  
Author(s):  
Fumiya Tao ◽  
Hirotaka Mihara ◽  
Nobuhiko Kojima
Keyword(s):  
Hepatic Tissue ◽  
Multicellular Spheroid ◽  
Spheroid Culture ◽  
Tissue Structures

scholarly journals The Use of Human Tumor Cells Grown in Multicellular Spheroid Culture for Designing and Improving Therapeutic Strategies

1998 ◽  
Vol 1 (3) ◽  
pp. 193-207 ◽  
Author(s):  
H. Acker
Keyword(s):  
Tumor Cells ◽  
Tumor Therapy ◽  
Human Tumor ◽  
Therapeutic Strategies ◽  
Multicellular Spheroid ◽  
Concerted Action ◽  
Human Tumor Cells ◽  
Spheroid Culture ◽  
Ideal System ◽  
Heterogeneous Interaction

Human turnor cells grown in threedimensional multicellular spheriod culture represent an ideal system to study the heterogeneous interaction between microenvironment and different therpeutic modalities in patients. Overcoming this hetergenenity is the most challeging task for the improvement of cancer theraphy. Examples of this interaction are given with the meaning of pO2and pH for the treatment of tumor cells with radition cytostatic,cytokines and gene therapy. The presented result might form a base for the use of muticellular spheriods in a concerted action between clinicians, biologist and mathematicians to improve tumor therapy individually for patient.

scholarly journals Effects of cobalt on haem proteins of erythropoietin-producing HepG2 cells in multicellular spheroid culture

FEBS Letters ◽  
1994 ◽  
Vol 348 (2) ◽  
pp. 216-218 ◽  
Author(s):  
A. Görlach ◽  
J. Fandrey ◽  
G. Holtermann ◽  
H. Acker
Keyword(s):  
Hepg2 Cells ◽  
Multicellular Spheroid ◽  
Spheroid Culture ◽  
Haem Proteins

scholarly journals Multicellular Spheroid Culture Models: Applications in prostate Cancer Research and Therapeutics

2011 ◽  
Vol 03 (03) ◽  
Author(s):  
Daisuke Kurioka ◽  
Akimitsu Takagi ◽  
Misao Yoneda ◽  
Yoshifumi Hirokawa ◽  
Taizo Shiraishi
Keyword(s):  
Prostate Cancer ◽  
Cancer Research ◽  
Multicellular Spheroid ◽  
Spheroid Culture ◽  
Culture Models ◽  
Prostate Cancer Research

Influence of glucose on metabolism and growth of rat glioma cells (C6) in multicellular spheroid culture

1992 ◽  
Vol 52 (2) ◽  
pp. 279-285 ◽  
Author(s):  
H. Acker ◽  
G. Holtermann ◽  
B. Bölling ◽  
J. Carlsson
Keyword(s):  
Glioma Cells ◽  
Multicellular Spheroid ◽  
Spheroid Culture ◽  
Rat Glioma

A Comparison of the Ultrastructural Morphology of Hepatic Necrosis in BDF1 Mice

1981 ◽  
Vol 39 ◽  
pp. 586-587
Author(s):  
Becky Jackson
Keyword(s):  
Endoplasmic Reticulum ◽  
Smooth Endoplasmic Reticulum ◽  
Preliminary Investigation ◽  
Hepatic Necrosis ◽  
Hepatic Tissue ◽  
Pronounced Increase ◽  
Glycogen Store ◽  
Ultrastructural Morphology ◽  
Mitochondrial Integrity ◽  
Glycogen Stores

Preliminary investigation has indicated similarity in hepatic ultrastructural morphology in nutritional deprivation, and cyanide induced hepatic necrosis. Analysis of hepatic tissue has indicated disruption of intracellular membranes, specifically, reduction in rough endoplasmic reticulum (RER) mitochondrial integrity, and glycogen stores. An increase in smooth endoplasmic reticulum (SER) portion was observed.To further investigate the apparent equivalence of necrotic morphology, ultrastructura1ly, BDF1 mice were subjected to senescence, nutritional deprevation, potassium cyanide (KCN) induced toxemia, and acetaminophen induced toxemia. Controls were utilized to ellucidate non-necrotic hepatocellular normals. U1trastructura1 investigation of controls (Fig. 1) shows densely granular RER, abundant glycogen stores, and morphologically normal mitochondria. Subjects with acetaminophen induced necrosis exhibit reduced normal RER with increased levels of dialated, vesicular RER in apparent conversion to SER (Fig. 2), loss of mitochondrial integrity, and glycogen store reduction. Senescent subjects exhibit a pronounced increase in SER and loss of glycogen store. (Fig. 3). Investigation of the senescent SER at high magnification (Fig. 5) indicates that the SER is arising from degranulating and vesiculating RER.

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