Reconstruction of Hepatic Tissue Structures Using Interstitial Flow in a Microfluidic Device

2018 ◽  
pp. 167-174 ◽  
Author(s):  
Ryo Sudo
Keyword(s):  
Microfluidic Device ◽  
Hepatic Tissue ◽  
Interstitial Flow ◽  
Tissue Structures

scholarly journals Microfluidic device to control interstitial flow-mediated homotypic and heterotypic cellular communication

Lab on a Chip ◽  
2015 ◽  
Vol 15 (17) ◽  
pp. 3521-3529 ◽  
Author(s):  
Luis F. Alonzo ◽  
Monica L. Moya ◽  
Venktesh S. Shirure ◽  
Steven C. George
Keyword(s):  
Microfluidic Device ◽  
Three Dimensional ◽  
Cellular Communication ◽  
Interstitial Flow ◽  
Temporal Interactions

To address the gap between in vivo microenvironments and in vitro systems, we have developed a novel microfluidic device that precisely controls the spatial and temporal interactions between adjacent three-dimensional cellular environments.

INTERSTITIAL FLOW ENHANCES FORMATION, CONNECTIVITY, AND FUNCTION OF 3D BRAIN MICROVASCULAR NETWORKS GENERATED WITHIN MICROFLUIDIC DEVICE

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Max A Winkelman ◽  
Diana Y Kim ◽  
Shravani Kakarla ◽  
Alexander Grath ◽  
Nathaniel Silvia ◽  
...  
Keyword(s):  
Blood Brain Barrier ◽  
Microfluidic Device ◽  
Convective Transport ◽  
Brain Barrier ◽  
Human Biology ◽  
Interstitial Flow ◽  
Microvascular Networks ◽  
Blood Brain ◽  
And Function

The convective transport of fluid through tissue is an important factor in human biology, including the development of brain microvascular networks (MVNs) with a functional blood-brain barrier (BBB). Engineering a...

A Comparison of the Ultrastructural Morphology of Hepatic Necrosis in BDF1 Mice

1981 ◽  
Vol 39 ◽  
pp. 586-587
Author(s):  
Becky Jackson
Keyword(s):  
Endoplasmic Reticulum ◽  
Smooth Endoplasmic Reticulum ◽  
Preliminary Investigation ◽  
Hepatic Necrosis ◽  
Hepatic Tissue ◽  
Pronounced Increase ◽  
Glycogen Store ◽  
Ultrastructural Morphology ◽  
Mitochondrial Integrity ◽  
Glycogen Stores

Preliminary investigation has indicated similarity in hepatic ultrastructural morphology in nutritional deprivation, and cyanide induced hepatic necrosis. Analysis of hepatic tissue has indicated disruption of intracellular membranes, specifically, reduction in rough endoplasmic reticulum (RER) mitochondrial integrity, and glycogen stores. An increase in smooth endoplasmic reticulum (SER) portion was observed.To further investigate the apparent equivalence of necrotic morphology, ultrastructura1ly, BDF1 mice were subjected to senescence, nutritional deprevation, potassium cyanide (KCN) induced toxemia, and acetaminophen induced toxemia. Controls were utilized to ellucidate non-necrotic hepatocellular normals. U1trastructura1 investigation of controls (Fig. 1) shows densely granular RER, abundant glycogen stores, and morphologically normal mitochondria. Subjects with acetaminophen induced necrosis exhibit reduced normal RER with increased levels of dialated, vesicular RER in apparent conversion to SER (Fig. 2), loss of mitochondrial integrity, and glycogen store reduction. Senescent subjects exhibit a pronounced increase in SER and loss of glycogen store. (Fig. 3). Investigation of the senescent SER at high magnification (Fig. 5) indicates that the SER is arising from degranulating and vesiculating RER.

The Glissonian Approach of the Hilum

Swiss Surgery ◽  
1999 ◽  
Vol 5 (3) ◽  
pp. 143-146 ◽  
Author(s):  
Launois ◽  
Maddern ◽  
Tay
Keyword(s):  
Portal Vein ◽  
Hepatic Artery ◽  
Posterior Approach ◽  
Hepatic Duct ◽  
Rapid Evolution ◽  
Hepatic Tissue ◽  
Detailed Knowledge ◽  
Porta Hepatis ◽  
Liver Segment ◽  
Glissonian Approach

The detailed knowledge of the segmental anatomy of the liver has led to a rapid evolution in resectional surgery based on the intrahepatic distribution of the portal trinity (the hepatic artery, hepatic duct and portal vein). The classical intrafascial or extrahepatic approach is to isolate the appropriate branch of the portal vein, hepatic artery and the hepatic duct, outside the liver substance. Another method, the extrafascial approach, is to dissect the whole sheath of the pedicle directly after division of a substantial amount of the hepatic tissue to reach the pedicle, which is surrounded by a sheath, derived from Glisson's capsule. This Glissonian sheath encloses the portal trinity. In the transfissural or intrahepatic approach, these sheaths can be approached either anteriorly (after division of the main, right or umbilical fissure) or posteriorly from behind the porta hepatis. We describe the technique for approaching the Glissonian sheath and hence the hepatic pedicle structures and their branches by the intrahepatic posterior approach that allows early delineation of the liver segment without the need for ancillary techniques. In addition, the indications for the use of this technique in the technical and oncologic settings are also discussed.

Increased frequencies of activated effector CD4+ T cells in the peripheral blood and hepatic tissue of patients with NAFL and NASH

2014 ◽  
Vol 52 (08) ◽  
Author(s):  
M Rau ◽  
AK Schilling ◽  
J Meertens ◽  
I Hering ◽  
T Kudlich ◽  
...  
Keyword(s):  
T Cells ◽  
Peripheral Blood ◽  
Cd4 T Cells ◽  
Hepatic Tissue

Pattern Recognition in Histo-Pathology: Basic Considerations

1982 ◽  
Vol 21 (01) ◽  
pp. 15-22 ◽  
Author(s):  
W. Schlegel ◽  
K. Kayser
Keyword(s):  
Pattern Recognition ◽  
Graph Theory ◽  
Normal Tissue ◽  
Diagnostic Procedures ◽  
Minimal Distance ◽  
Malignant Growth ◽  
Colon Tissue ◽  
First Results ◽  
Tissue Structures ◽  
The Given

A basic concept for the automatic diagnosis of histo-pathological specimen is presented. The algorithm is based on tissue structures of the original organ. Low power magnification was used to inspect the specimens. The form of the given tissue structures, e. g. diameter, distance, shape factor and number of neighbours, is measured. Graph theory is applied by using the center of structures as vertices and the shortest connection of neighbours as edges. The algorithm leads to two independent sets of parameters which can be used for diagnostic procedures. First results with colon tissue show significant differences between normal tissue, benign and malignant growth. Polyps form glands that are twice as wide as normal and carcinomatous tissue. Carcinomas can be separated by the minimal distance of the glands formed. First results of pattern recognition using graph theory are discussed.

Sign in / Sign up

Export Citation Format

Share Document