Generation of Neural Crest Progenitors from Human Pluripotent Stem Cells

2019 ◽  
pp. 37-47 ◽  
Author(s):  
Kwaku Dad Abu-Bonsrah ◽  
Serena Viventi ◽  
Donald F. Newgreen ◽  
Mirella Dottori
Keyword(s):  
Stem Cells ◽  
Neural Crest ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells

scholarly journals Notch signaling regulates the differentiation of neural crest from human pluripotent stem cells

Development ◽  
2014 ◽  
Vol 141 (11) ◽  
pp. e1106-e1106
Author(s):  
P. Noisa ◽  
C. Lund ◽  
K. Kanduri ◽  
R. Lund ◽  
H. Lahdesmaki ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Crest ◽  
Notch Signaling ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells

scholarly journals Differentiation protocol for cranial neural crest-derived pericyte-like cells from human pluripotent stem cells

2020 ◽  
Author(s):  
Jiaqi Sun ◽  
Yinong Huang ◽  
Jin Gong ◽  
Yili Wei ◽  
Chuanfeng Xiong ◽  
...  
Keyword(s):  
Stem Cells ◽  
Ischemic Stroke ◽  
Neural Crest ◽  
Human Embryonic Stem Cells ◽  
Pluripotent Stem Cells ◽  
Embryonic Stem ◽  
Human Pluripotent Stem Cells ◽  
Cranial Neural Crest ◽  
Differentiation Protocol ◽  
Induced Pluripotent

Abstract The successful differentiation of brain pericyte-like cells from human pluripotent stem cells may allow us to study their biological characteristics and their applications in the treatment of pericyte dysfunction-related neurodegenerative diseases, including ischemic stroke, Parkinson’s disease (PD) and so on.The protocol we present in the study provides a cranial neural crest originated, fast and robust forebrain pericyte-like cells differentiation method using either human embryonic stem cells or human induced pluripotent stem cells as a starting material.

scholarly journals Brainstem organoids from human pluripotent stem cells contain neural crest population

2019 ◽  
Author(s):  
Nobuyuki Eura ◽  
Takeshi K. Matsui ◽  
Joachim Luginbühl ◽  
Masaya Matsubayashi ◽  
Hitoki Nanaura ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Crest ◽  
Pluripotent Stem Cells ◽  
Dopaminergic Neurons ◽  
Cholinergic Neurons ◽  
Human Pluripotent Stem Cells ◽  
Neural Crest Stem Cells ◽  
Human Brain Development ◽  
Cellular Population ◽  
The Brain

SummaryThe brainstem controls heartbeat, blood pressure and respiration, which are life-sustaining functions, therefore, disorders of the brainstem can be lethal. Brain organoids derived from human pluripotent stem cells recapitulate the course of human brain development and are expected to be useful for medical research on central nervous system disorders. However, existing organoid models have limitations, hampering the elucidation of diseases affecting specific components of the brain. Here, we developed a method to generate human brainstem organoids (hBSOs), containing neural crest stem cells as well as midbrain/hindbrain progenitors, noradrenergic and cholinergic neurons, and dopaminergic neurons, demonstrated by specific electrophysiological signatures. Single-cell RNA sequence analysis, together with proteomics and electrophysiology, revealed that the cellular population in these organoids was similar to that of the human brainstem and neural crest, which raises the possibility of making use of hBSOs in grafting for transplantation, efficient drug screenings and modeling the neural crest diseases.

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