ESCRT Mutant Analysis and Imaging of ESCRT Components in the Model Fungus Ustilago maydis

2019 ◽  
pp. 251-271 ◽  
Author(s):  
Carl Haag ◽  
Thomas Klein ◽  
Michael Feldbrügge
Keyword(s):  
Ustilago Maydis ◽  
Mutant Analysis

Ustilago maydis Virulence Assays in Maize

BIO-PROTOCOL ◽  
2016 ◽  
Vol 6 (6) ◽  
Author(s):  
Amey Redkar ◽  
Gunther Doehlemann
Keyword(s):  
Ustilago Maydis

scholarly journals Ca2+-ATPase-driven calcium accumulation in Ustilago maydis plasma membrane vesicles

Microbiology ◽  
1994 ◽  
Vol 140 (11) ◽  
pp. 3047-3051 ◽  
Author(s):  
A. Hernandez ◽  
D. T. Cooke ◽  
D. T. Clarkson
Keyword(s):  
Plasma Membrane ◽  
Membrane Vesicles ◽  
Ustilago Maydis ◽  
Plasma Membrane Vesicles ◽  
Calcium Accumulation

scholarly journals Zfp1, a putative Zn(II) 2 Cys 6 transcription factor, influences Ustilago maydis pathogenesis at multiple stages

2021 ◽  
Author(s):  
H.Y. Kitty Cheung ◽  
Michael E. Donaldson ◽  
Emilee R.M. Storfie ◽  
Kelsey L. Spence ◽  
Jessie L.O. Fetsch ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Ustilago Maydis ◽  
Multiple Stages

scholarly journals Controlling Unconventional Secretion for Production of Heterologous Proteins in Ustilago maydis through Transcriptional Regulation and Chemical Inhibition of the Kinase Don3

2021 ◽  
Vol 7 (3) ◽  
pp. 179
Author(s):  
Kai P. Hussnaetter ◽  
Magnus Philipp ◽  
Kira Müntjes ◽  
Michael Feldbrügge ◽  
Kerstin Schipper
Keyword(s):  
Protein Production ◽  
Heterologous Protein ◽  
Ustilago Maydis ◽  
Downstream Processing ◽  
Culture Broth ◽  
Yeast Cells ◽  
Heterologous Protein Expression ◽  
Heterologous Proteins ◽  
Regulatory Systems ◽  
Unconventional Secretion

Heterologous protein production is a highly demanded biotechnological process. Secretion of the product to the culture broth is advantageous because it drastically reduces downstream processing costs. We exploit unconventional secretion for heterologous protein expression in the fungal model microorganism Ustilago maydis. Proteins of interest are fused to carrier chitinase Cts1 for export via the fragmentation zone of dividing yeast cells in a lock-type mechanism. The kinase Don3 is essential for functional assembly of the fragmentation zone and hence, for release of Cts1-fusion proteins. Here, we are first to develop regulatory systems for unconventional protein secretion using Don3 as a gatekeeper to control when export occurs. This enables uncoupling the accumulation of biomass and protein synthesis of a product of choice from its export. Regulation was successfully established at two different levels using transcriptional and post-translational induction strategies. As a proof-of-principle, we applied autoinduction based on transcriptional don3 regulation for the production and secretion of functional anti-Gfp nanobodies. The presented developments comprise tailored solutions for differentially prized products and thus constitute another important step towards a competitive protein production platform.

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