Isolation and Purification of Rodent Pancreatic Islets of Langerhans

2019 ◽  
pp. 179-184
Author(s):  
Jacqueline F. O’Dowd ◽  
Claire J. Stocker
Keyword(s):  
Pancreatic Islets ◽  
Islets Of Langerhans ◽  
Isolation And Purification ◽  
Pancreatic Islets Of Langerhans

Altered TNF-α, glucose, insulin, and amino acids in islets of Langerhans cultured in a microgravity model system

2001 ◽  
Vol 280 (1) ◽  
pp. E92-E102 ◽  
Author(s):  
Brian W. Tobin ◽  
Sandra K. Leeper-Woodford ◽  
Brian B. Hashemi ◽  
Scott M. Smith ◽  
Clarence F. Sams
Keyword(s):  
Cell Culture ◽  
Pancreatic Islets ◽  
Islets Of Langerhans ◽  
Three Dimensional ◽  
Model System ◽  
Culture Methods ◽  
Static Culture ◽  
Pancreatic Islets Of Langerhans ◽  
Tnf Α ◽  
Factor Α

The present studies were designed to determine effects of a microgravity model system upon lipopolysaccharide (LPS)-stimulated tumor necrosis factor-α (TNF-α) activity and indexes of insulin and fuel homeostasis of pancreatic islets of Langerhans. Islets (1,726 ± 117, 150 islet equivalent units) from Wistar-Furth rats were treated as 1) high aspect ratio vessel (HARV) cell culture, 2) HARV plus LPS, 3) static culture, and 4) static culture plus LPS. TNF-α (L929 cytotoxicity assay) was significantly increased in LPS-induced HARV and static cultures; yet the increase was more pronounced in the static culture group ( P < 0.05). A decrease in insulin concentration was demonstrated in the LPS-stimulated HARV culture ( P< 0.05). We observed a greater glucose concentration and increased disappearance of arginine in islets cultured in HARVs. Although nitrogenous compound analysis indicated a ubiquitous reliance on glutamine in all experimental groups, arginine was converted to ornithine at a twofold greater rate in the islets cultured in the HARV microgravity model system ( P < 0.05). These studies demonstrate alterations in LPS-induced TNF-α production of pancreatic islets of Langerhans, favoring a lesser TNF activity in the HARV. These alterations in fuel homeostasis may be promulgated by gravity-averaged cell culture methods or by three-dimensional cell assembly.

A method for large-scale, high-yield isolation of canine pancreatic islets of Langerhans

Metabolism ◽  
1982 ◽  
Vol 31 (2) ◽  
pp. 184-187 ◽  
Author(s):  
Jack Noel ◽  
Alexander Rabinovitch ◽  
Les Olson ◽  
George Kyriakides ◽  
Joshua Miller ◽  
...  
Keyword(s):  
Pancreatic Islets ◽  
Islets Of Langerhans ◽  
Large Scale ◽  
High Yield ◽  
Pancreatic Islets Of Langerhans

SURVIVAL OF CRYOPRESERVED ISOLATED ADULT HUMAN PANCREATIC ISLETS OF LANGERHANS

1987 ◽  
Vol 44 (1) ◽  
pp. 75-82 ◽  
Author(s):  
GARTH L. WARNOCK ◽  
DEREK W. R. GRAY ◽  
PHILIP MCSHANE ◽  
MAUREEN PETERS ◽  
PETER J. MORRIS
Keyword(s):  
Pancreatic Islets ◽  
Islets Of Langerhans ◽  
Human Pancreatic Islets ◽  
Adult Human ◽  
Pancreatic Islets Of Langerhans
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