Protein Extraction From Mammalian Tissues

2007 ◽  
pp. 385-389 ◽  
Author(s):  
Choogon Lee
Keyword(s):  
Protein Extraction ◽  
Mammalian Tissues

Discovery of intracellular 2,8 dihydroxyadenine crystals in bovine lymphatic and hepatic cells

1987 ◽  
Vol 45 ◽  
pp. 906-907
Author(s):  
W. E. Rigsby ◽  
D. M. Hinton ◽  
V. J. Hurst ◽  
P. C. McCaskey
Keyword(s):  
Polarized Light ◽  
Paraffin Sections ◽  
Tissue Samples ◽  
Whole Cells ◽  
Tissue Sections ◽  
Hepatic Cells ◽  
Slaughter House ◽  
Mammalian Tissues ◽  
Carbon Coated ◽  
Cellular Components

Crystalline intracellular inclusions are rarely seen in mammalian tissues and are often difficult to positively identify. Lymph node and liver tissue samples were obtained from two cows which had been rejected at the slaughter house due to the abnormal appearance of these organs in the animals. The samples were fixed in formaldehyde and some of the fixed material was embedded in paraffin. Examination of the paraffin sections with polarized light microscopy revealed the presence of numerous crystals in both hepatic and lymph tissue sections. Tissue sections were then deparaffinized in xylene, mounted, carbon coated, and examined in a Phillips 505T SEM equipped with a Tracor Northern X-ray Energy Dispersive Spectroscopy (EDS) system. Crystals were obscured by cellular components and membranes so that EDS spectra were only obtainable from whole cells. Tissue samples which had been fixed but not paraffin-embedded were dehydrated, embedded in Spurrs plastic, and sectioned.

Localization of endothelial nitric oxide synthase in the normal and failing human atrial myocardium

1996 ◽  
Vol 54 ◽  
pp. 786-787
Author(s):  
Chi-Ming Wei ◽  
Margarita Bracamonte ◽  
Shi-Wen Jiang ◽  
Richard C. Daly ◽  
Christopher G.A. McGregor ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Heart Failure ◽  
Nitric Oxide Synthase ◽  
Endothelial Nitric Oxide Synthase ◽  
Cardiac Tissues ◽  
Mammalian Tissues ◽  
End Stage Heart Failure ◽  
End Stage ◽  
Oxide Synthase ◽  
Endothelial Nitric Oxide

Nitric oxide (NO) is a potent endothelium-derived relaxing factor which also may modulate cardiomyocyte inotropism and growth via increasing cGMP. While endothelial nitric oxide synthase (eNOS) isoforms have been detected in non-human mammalian tissues, expression and localization of eNOS in the normal and failing human myocardium are poorly defined. Therefore, the present study was designed to investigate eNOS in human cardiac tissues in the presence and absence of congestive heart failure (CHF).Normal and failing atrial tissue were obtained from six cardiac donors and six end-stage heart failure patients undergoing primary cardiac transplantation. ENOS protein expression and localization was investigated utilizing Western blot analysis and immunohistochemical staining with the polyclonal rabbit antibody to eNOS (Transduction Laboratories, Lexington, Kentucky).

Verification of Protein Extraction Method by Magneto-Ferrite Treatment with EDS Analysis

2020 ◽  
Vol 140 (2) ◽  
pp. 128-129
Author(s):  
Suguru Kotani ◽  
Masaya Endo ◽  
Mahmudul Kabir ◽  
Kazutaka Mitobe
Keyword(s):  
Extraction Method ◽  
Protein Extraction ◽  
Eds Analysis

Methods of Protein Extraction from Soil and Their Potential Application inStudy of Soil Metaproteome

2012 ◽  
Vol 18 (4) ◽  
pp. 691
Author(s):  
Xiaoli WANG ◽  
PEREZ Pablo GONZALEZ ◽  
Jun YE ◽  
Danfeng HUANG
Keyword(s):  
Potential Application ◽  
Protein Extraction

Protein Extraction from Mice Xenograft Tumor

BIO-PROTOCOL ◽  
2012 ◽  
Vol 2 (7) ◽  
Author(s):  
Huan Pang
Keyword(s):  
Protein Extraction ◽  
Xenograft Tumor

Evidence for presence of a reduced form of digoxin-like immunoreactive factor (dihydro-DLIF) in mammalian tissues

1996 ◽  
Vol 42 (7) ◽  
pp. 1092-1099 ◽  
Author(s):  
H M Qazzaz ◽  
S A Jortani ◽  
J M Poole ◽  
R Valdes
Keyword(s):  
Lactone Ring ◽  
Fold Increase ◽  
Cross Reactivity ◽  
Reduced Form ◽  
Molar Ratio ◽  
Endogenous Ligand ◽  
Metabolic Route ◽  
Mammalian Tissues ◽  
Spectral Absorbance ◽  
Cytochrome P 450

Abstract Digoxin-like immunoreactive factor (DLIF) from adrenal glands is an endogenous ligand structurally related to the plant-derived cardiac glycoside digoxin. Cardiac glycosides regulate the activity of the sodium pump and thus play key roles in disease processes involving regulation of ion transport. We now report the discovery of an endogenous dihydro-DLIF analogous to dihydrodigoxin. We used HPLC, ultraviolet spectrophotometry, and cross-reactivity with two antibodies, one specific for digoxin and one for dihydrodigoxin, to support the hypothesis that dihydro-DLIF contains a chemically reduced lactone ring. The spectral absorbance maximum for dihydro-DLIF is at 196 nm, identical to dihydrodigoxin. DLIF and dihydro-DLIF are 975- and 2588-fold less immunoreactive than digoxin and dihydrodigoxin for their respective antibodies. The molar ratio of dihydro-DLIF to DLIF is approximately 5.3 in bovine adrenocortical tissue and approximately 0.38 in human serum. Dihydrodigoxin (reduced lactone ring) added to microsomes isolated from bovine adrenal cortex produced a 4.5-fold increase in digoxin-like immunoreactivity (oxidized lactone ring) after 3 h of incubation. The biotransformation is likely mediated by a cytochrome P-450 NADPH-dependent process. Our findings demonstrate the presence of a dihydro-DLIF in mammals and suggest a metabolic route for synthesis of endogenous DLIF in mammalian tissue.

scholarly journals An improved protein extraction method applied to cotton leaves is compatible with 2-DE and LC-MS

BMC Genomics ◽  
2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Xiang Jin ◽  
Liping Zhu ◽  
Chengcheng Tao ◽  
Quanliang Xie ◽  
Xinyang Xu ◽  
...  
Keyword(s):  
Extraction Method ◽  
Protein Extraction
Sign in / Sign up

Export Citation Format

Share Document