Use of Real-Time Polymerase Chain Reaction to Measure Gene Expression in Single Cells

2007 ◽  
pp. 125-133 ◽  
Author(s):  
Dagan Wells
Keyword(s):  
Gene Expression ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Single Cells ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Measure Gene Expression

scholarly journals Expression of DROSHA and DICER genes in peripheral blood leukocytes in lung sarcoidosis

2018 ◽  
Vol 90 (3) ◽  
pp. 21-24
Author(s):  
I E Malysheva ◽  
O V Balan ◽  
E L Tikhonovich ◽  
T O Volkova
Keyword(s):  
Gene Expression ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Peripheral Blood ◽  
Messenger Rna ◽  
Peripheral Blood Leukocytes ◽  
Chain Reaction ◽  
Blood Leukocytes ◽  
Healthy Donors ◽  
Polymerase Chain

Aim. To study the expression level of the genes DROSHA and DICER in peripheral blood leukocytes (PBL) of patients with sarcoidosis of the lungs Materials and methods. The study included 32 patients diagnosed with persistent lung sarcoidosis (mean age 41.56±1.27 years) and 36 healthy donors (control; mean age 42.79±1.95 years). The level of expression of messenger RNA (mRNA) of the genes DROSHA and DICER were determined in PBL of healthy donors and patients with sarcoidosis of the lung by polymerase chain reaction in real time. Results. As a result of the conducted researches it is established that the level of drosha gene expression in PBL patients with sarcoidosis of lungs is significantly reduced in comparison with the control (p

Comparison of real-time polymerase chain reaction and end-point polymerase chain reaction for the analysis of gene expression in preimplantation embryos

2006 ◽  
Vol 18 (3) ◽  
pp. 365 ◽  
Author(s):  
Árpád Baji Gál ◽  
Joseph Wallace Carnwath ◽  
Andras Dinnyes ◽  
Doris Herrmann ◽  
Heiner Niemann ◽  
...  
Keyword(s):  
Gene Expression ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Reverse Transcription ◽  
Dynamic Range ◽  
Chain Reaction ◽  
Real Time System ◽  
The Real ◽  
End Point ◽  
Polymerase Chain

The aim of the present study was to compare real-time polymerase chain reaction (PCR) and end-point PCR with respect to their suitability for the analysis of gene expression in samples in which the number of cells is limited; for example, in studies of preimplantation embryonic development and to determine the variability of the real-time reverse transcription–PCR assay. The sensitivity, dynamic range and precision of both PCR systems were compared using a single mouse liver cDNA standard. The real-time system was 100-fold more sensitive than the end-point system and had a dynamic range of more than four orders of magnitude. The linear range for end-point PCR extended for two orders of magnitude using a fixed end-point of 31 cycles. The percentage standard error of the mean based on 30 replicates was 0.14% of the threshold cycle (Ct) value for the real-time system and 6.8% for the end-point fluorescence intensity. The coefficients of variation (CV) for reverse transcription combined with real-time analysis and the complete gene expression protocol consisting of mRNA isolation, reverse transcription and real-time PCR analysis were 0.6% and 1.4% of the Ct values, respectively. The present paper details, for the first time, measurement of the biological variation of individual mammalian oocytes. The CV was 1.8% of the Ct value for expression analysis of six bovine oocytes. The results are discussed in relation to the analysis of gene expression in preimplantation embryo development.

Quantitative assessment of gene expression in highly purified hematopoietic cells using real-time reverse transcriptase polymerase chain reaction

2002 ◽  
Vol 30 (5) ◽  
pp. 481-487 ◽  
Author(s):  
Marc H.G.P. Raaijmakers ◽  
Liesbeth van Emst ◽  
Theo de Witte ◽  
Ewald Mensink ◽  
Reinier A.P. Raymakers
Keyword(s):  
Gene Expression ◽  
Polymerase Chain Reaction ◽  
Reverse Transcriptase ◽  
Real Time ◽  
Quantitative Assessment ◽  
Hematopoietic Cells ◽  
Chain Reaction ◽  
Polymerase Chain

Comparison of reference genes for quantitative real-time polymerase chain reaction analysis of gene expression in sugarcane

2004 ◽  
Vol 22 (4) ◽  
pp. 325-337 ◽  
Author(s):  
Hayati M. Iskandar ◽  
Robert S. Simpson ◽  
Rosanne E. Casu ◽  
Graham D. Bonnett ◽  
Donald J. Maclean ◽  
...  
Keyword(s):  
Gene Expression ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Reference Genes ◽  
Polymerase Chain Reaction Analysis ◽  
Chain Reaction ◽  
Polymerase Chain

Improved Biplex Quantitative Real-Time Polymerase Chain Reaction with Modified Primers for Gene Expression Analysis

2006 ◽  
Vol 16 (4) ◽  
pp. 395-403 ◽  
Author(s):  
Irina A. Afonina ◽  
Alan Mills ◽  
Silvia Sanders ◽  
Alena Kulchenko ◽  
Robert Dempcy ◽  
...  
Keyword(s):  
Gene Expression ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Chain Reaction ◽  
Polymerase Chain
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