Protein Stabilization by the Rational Design of Surface Charge–Charge Interactions

2008 ◽  
pp. 261-283 ◽  
Author(s):  
Katrina L. Schweiker ◽  
George I. Makhatadze
Keyword(s):  
Surface Charge ◽  
Rational Design ◽  
Protein Stabilization ◽  
Charge Interactions

Surface charge-based rational design of aspartase modifies the optimal pH for efficient β-aminobutyric acid production

2020 ◽  
Vol 164 ◽  
pp. 4165-4172
Author(s):  
Yaling Wang ◽  
Meijuan Xu ◽  
Taowei Yang ◽  
Xian Zhang ◽  
Zhiming Rao
Keyword(s):  
Surface Charge ◽  
Acid Production ◽  
Rational Design ◽  
Aminobutyric Acid ◽  
Optimal Ph

scholarly journals Improvement in Thermostability of an Achaetomium sp. Strain Xz8 Endopolygalacturonase via the Optimization of Charge-Charge Interactions

2015 ◽  
Vol 81 (19) ◽  
pp. 6938-6944 ◽  
Author(s):  
Tao Tu ◽  
Huiying Luo ◽  
Kun Meng ◽  
Yanli Cheng ◽  
Rui Ma ◽  
...  
Keyword(s):  
Rational Design ◽  
Residual Activity ◽  
Maximal Activity ◽  
Wild Type ◽  
Content Type ◽  
Highly Active ◽  
Wide Ph Range ◽  
Enzyme Thermal Stability ◽  
Ph Range ◽  
Charge Interactions

ABSTRACTImproving enzyme thermostability is of importance for widening the spectrum of application of enzymes. In this study, a structure-based rational design approach was used to improve the thermostability of a highly active, wide-pH-range-adaptable, and stable endopolygalacturonase (PG8fn) fromAchaetomiumsp. strain Xz8 via the optimization of charge-charge interactions. By using the enzyme thermal stability system (ETSS), two residues—D244 and D299—were inferred to be crucial contributors to thermostability. Single (D244A and D299R) and double (D244A/D299R) mutants were then generated and compared with the wild type. All mutants showed improved thermal properties, in the order D244A < D299R < D244A/D299R. In comparison with PG8fn, D244A/D299R showed the most pronounced shifts in temperature of maximum enzymatic activity (Tmax), temperature at which 50% of the maximal activity of an enzyme is retained (T50), and melting temperature (Tm), of about 10, 17, and 10.2°C upward, respectively, with the half-life (t1/2) extended by 8.4 h at 50°C and 45 min at 55°C. Another distinguishing characteristic of the D244A/D299R mutant was its catalytic activity, which was comparable to that of the wild type (23,000 ± 130 U/mg versus 28,000 ± 293 U/mg); on the other hand, it showed more residual activity (8,400 ± 83 U/mg versus 1,400 ± 57 U/mg) after the feed pelleting process (80°C and 30 min). Molecular dynamics (MD) simulation studies indicated that mutations at sites D244 and D299 lowered the overall root mean square deviation (RMSD) and consequently increased the protein rigidity. This study reveals the importance of charge-charge interactions in protein conformation and provides a viable strategy for enhancing protein stability.

scholarly journals Surface Charge Interactions of the FMN Module Govern Catalysis by Nitric-oxide Synthase

2006 ◽  
Vol 281 (48) ◽  
pp. 36819-36827 ◽  
Author(s):  
Koustubh Panda ◽  
Mohammad Mahfuzul Haque ◽  
Elsa D. Garcin-Hosfield ◽  
Deborah Durra ◽  
Elizabeth D. Getzoff ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Surface Charge ◽  
Oxide Synthase ◽  
Charge Interactions

scholarly journals Computational design of the Fyn SH3 domain with increased stability through optimization of surface charge-charge interactions

2007 ◽  
Vol 16 (12) ◽  
pp. 2694-2702 ◽  
Author(s):  
Katrina L. Schweiker ◽  
Arash Zarrine-Afsar ◽  
Alan R. Davidson ◽  
George I. Makhatadze
Keyword(s):  
Surface Charge ◽  
Computational Design ◽  
Sh3 Domain ◽  
Charge Interactions

Long-Range Surface Charge-Charge Interactions in Proteins

1993 ◽  
Vol 232 (2) ◽  
pp. 574-583 ◽  
Author(s):  
Reign Loewenthal ◽  
Javier Sancho ◽  
Tapani Reinikainen ◽  
Alan R. Fersht
Keyword(s):  
Surface Charge ◽  
Long Range ◽  
Charge Interactions

A Zeta Potentiometric Study on Effects of Ionic Composition and Rock-Saturation on Surface-Charge Interactions in Low-Salinity Water Flooding for Carbonate Formation

2021 ◽  
Author(s):  
Paras Himmat Gopani ◽  
Navpreet Singh ◽  
Hemanta Kumar Sarma ◽  
Digambar S. Negi ◽  
Padmaja S. Mattey
Keyword(s):  
Zeta Potential ◽  
Surface Charge ◽  
Carbonate Rocks ◽  
Carbonate Rock ◽  
Ionic Composition ◽  
Streaming Potential ◽  
Potentiometric Studies ◽  
Rock Samples ◽  
Streaming Current ◽  
Charge Interactions

Abstract As carbonate reservoirs are mostly oil-wet, the potential for the success of a waterflooding is lower. Therefore, a primary focus during waterflooding such reservoirs is on the ionic composition and salinity of injected brine which are able to impact the alteration of the rock wettability favorably by altering the surface charge towards a higher negative value or close to zero. The objective of this study is to employ zeta potentiometric studies comprising streaming potential and streaming current techniques to quantify the surface interactions and charges between the carbonate rock and fluid type as a function of the variations in its ionic state and rock saturation. Zeta potentiometric studies were conducted on carbonate rock samples to understand the behavior of different aqueous solutions by variation in the brine's salinity and ionic composition and the results were integrated with wettability studies. The concentrations of potential-determining ions (PDIs) such as SO42-, Mg2+ and Ca2+ in the injected brines are deemed responsible for altering the wettability state of the carbonate rocks. Several diluted brines (25%, 10% and 1% diluted seawater) and smart brines have been investigated. Smart brines were prepared by spiking the concentration of major PDIs. All zeta potential measurements were conducted using a specially designed zeta potentiometer sample-holding clamp capable of using the whole core plugs rather than pulverized rock samples. A major advantage of using the whole core sample is that the same core can be used in subsequent coreflooding tests, thus making zeta potentiometric results more relevant and representative for a particular rock-fluid system used in the study. The classical streaming potential and streaming current techniques were used for zeta potential measurement. The Fairbrother-Mastin approach was used where the streaming potential is measured against different pressure differentials. Measurements were also carried out for brines with rock samples of different states: oil-saturated, water-saturated and rock samples cleaned with organic solvents to determine any likely variations in surface charge interactions. The results of our experiments imply that the value of zeta potential either increases or becomes more negative with increasing percentage of dilution (25%, 10%, and 1%). This can be attributed to electrical double-layer expansion which is primarily caused by reduced ionic strength. Furthermore, with measurements done on smart brines, zeta potential value was also found to be increased when different diluted brines are spiked with ionic concentration of PDIs such as sulfate. This could have been caused by surface ion alteration mechanism where PDIs get adsorbed on rock surface causing possible detachment of oil droplets. Both the phenomena are known mechanisms for altering wettability towards more water wetness in carbonate rocks and are discussed in detail.

Rational design of electrocatalysts for simultaneously promoting bulk charge separation and surface charge transfer in solar water splitting photoelectrodes

2018 ◽  
Vol 6 (6) ◽  
pp. 2568-2576 ◽  
Author(s):  
Yingfei Hu ◽  
Yangqing Wu ◽  
Jianyong Feng ◽  
Huiting Huang ◽  
Chunchen Zhang ◽  
...  
Keyword(s):  
Charge Transfer ◽  
Surface Charge ◽  
Water Splitting ◽  
Thermodynamic Functions ◽  
Charge Separation ◽  
Rational Design ◽  
Solar Water ◽  
Solar Water Splitting ◽  
Bulk Charge

The composite AgOx/NiOx electrocatalyst was found to possess both kinetic and thermodynamic functions.

scholarly journals Rational Design of a Synthetic Peg-Like Polymer for Protein Stabilization

2017 ◽  
Vol 112 (3) ◽  
pp. 59a
Author(s):  
Christopher DelRe ◽  
Brian Panganiban ◽  
Tim Li ◽  
Charley Huang ◽  
Monica Olvera de la Cruz ◽  
...  
Keyword(s):  
Rational Design ◽  
Protein Stabilization

scholarly journals Removal of surface charge-charge interactions from ubiquitin leaves the protein folded and very stable

2008 ◽  
Vol 11 (1) ◽  
pp. 174-177 ◽  
Author(s):  
Vakhtang V. Loladze ◽  
George I. Makhatadze
Keyword(s):  
Surface Charge ◽  
Charge Interactions
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