The Albino Xenopus laevis Tadpole as a Novel Model of Developmental Seizures

2009 ◽  
pp. 45-57
Author(s):  
D. Sesath Hewapathirane ◽  
Kurt Haas
Keyword(s):  
Xenopus Laevis ◽  
Novel Model ◽  
Xenopus Laevis Tadpole

scholarly journals Expression of the Ca2+-binding protein, parvalbumin, during embryonic development of the frog, Xenopus laevis

1987 ◽  
Vol 104 (4) ◽  
pp. 841-847 ◽  
Author(s):  
BK Kay ◽  
AJ Shah ◽  
WE Halstead
Keyword(s):  
Xenopus Laevis ◽  
Fusion Protein ◽  
Binding Protein ◽  
Expression Library ◽  
Simple Method ◽  
High Affinity ◽  
Tadpole Tail ◽  
Developing Muscle ◽  
Beta Galactosidase ◽  
Xenopus Laevis Tadpole

A cDNA segment encoding the Ca2+-binding protein, parvalbumin, was isolated with the use of antibodies, from a lambda gtll expression library of Xenopus laevis tadpole poly(A)+ RNAs. The bacterially expressed beta-galactosidase-parvalbumin fusion protein of one lambda recombinant shows high affinity 45Ca2+ binding. The sequence of the tadpole parvalbumin is highly similar to previously characterized beta-parvalbumins of other organisms. Data from protein and RNA blotting experiments demonstrate that parvalbumin is absent in oocytes, eggs, and early staged embryos, and only becomes expressed during embryogenesis at the time of myogenesis. The protein can be detected in individual developing muscle cells and in muscle fibers of tadpole tail muscles. A simple method is also described for the isolation of neural tube-notochord-somite complexes from Xenopus embryos.

Specific cell surface labels in the visual centers of Xenopus laevis tadpole identified using monoclonal antibodies

1987 ◽  
Vol 122 (1) ◽  
pp. 90-100 ◽  
Author(s):  
Shin Takagi ◽  
Toshiaki Tsuji ◽  
Takashi Amagai ◽  
Tetsuro Takamatsu ◽  
Hajime Fujisawa
Keyword(s):  
Monoclonal Antibodies ◽  
Cell Surface ◽  
Xenopus Laevis ◽  
Specific Cell ◽  
Specific Cell Surface ◽  
Xenopus Laevis Tadpole

scholarly journals Use of Large-Scale Expression Cloning Screens in the Xenopus laevis Tadpole to Identify Gene Function

2000 ◽  
Vol 228 (2) ◽  
pp. 197-210 ◽  
Author(s):  
Timothy C Grammer ◽  
Karen J Liu ◽  
Francesca V Mariani ◽  
Richard M Harland
Keyword(s):  
Xenopus Laevis ◽  
Gene Function ◽  
Large Scale ◽  
Expression Cloning ◽  
Xenopus Laevis Tadpole

scholarly journals Differential expression of the Xenopus laevis tadpole and adult beta-globin genes when injected into fertilized Xenopus laevis eggs.

1984 ◽  
Vol 4 (3) ◽  
pp. 567-570 ◽  
Author(s):  
M M Bendig ◽  
J G Williams
Keyword(s):  
Xenopus Laevis ◽  
Differential Expression ◽  
Maximum Level ◽  
Globin Genes ◽  
Beta Globin ◽  
Stages Of Development ◽  
Chromosomal Genes ◽  
Xenopus Laevis Tadpole

Xenopus laevis tadpole and adult beta-globin genes were injected into fertilized X. laevis eggs. Both injected genes replicated and were retained in the developing embryos with equal efficiency. Transcripts of the injected adult gene were detectable at gastrulation and reached a maximum level shortly thereafter. In contrast, transcripts of the injected tadpole gene were not detected until much later stages of development. The level of expression of both the injected genes was low compared with the level of expression of the chromosomal genes during erythropoiesis.

scholarly journals Novel Behavioral Assays to Model Neurodevelopmental Disorders in the Xenopus laevis Tadpole

2015 ◽  
Vol 29 (S1) ◽  
Author(s):  
Carolina Ramirez‐Vizcarrondo ◽  
Mashfiq Hasan ◽  
Jenny Gu ◽  
Arseny Khakhalin ◽  
Carlos Aizenman
Keyword(s):  
Xenopus Laevis ◽  
Neurodevelopmental Disorders ◽  
Behavioral Assays ◽  
Xenopus Laevis Tadpole
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