Cell Synchrony Techniques

1987 ◽  
pp. 367-402 ◽  
Author(s):  
David J. Grdina ◽  
Marvin L. Meistrich ◽  
Raymond E. Meyn ◽  
Tod S. Johnson ◽  
R. Allen White
Keyword(s):  
Cell Synchrony

Cell Synchrony Techniques. I. A Comparison of Methods

1984 ◽  
Vol 17 (3) ◽  
pp. 223-236 ◽  
Author(s):  
David J. Grdina ◽  
Marvin L. Meistrich ◽  
Raymond E. Meyn ◽  
Tod S. Johnson ◽  
R. Allen Whitel
Keyword(s):  
Comparison Of Methods ◽  
Cell Synchrony

scholarly journals Role for E2F in Control of Both DNA Replication and Mitotic Functions as Revealed from DNA Microarray Analysis

2001 ◽  
Vol 21 (14) ◽  
pp. 4684-4699 ◽  
Author(s):  
Seiichi Ishida ◽  
Erich Huang ◽  
Harry Zuzan ◽  
Rainer Spang ◽  
Gustavo Leone ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Gene Regulation ◽  
Dna Replication ◽  
Dna Microarrays ◽  
Cell Cycle Analysis ◽  
Cell Synchrony ◽  
Mammalian Cell Cycle ◽  
Induced Genes ◽  
Regulated Gene Expression ◽  
E2f Proteins

ABSTRACT We have used high-density DNA microarrays to provide an analysis of gene regulation during the mammalian cell cycle and the role of E2F in this process. Cell cycle analysis was facilitated by a combined examination of gene control in serum-stimulated fibroblasts and cells synchronized at G1/S by hydroxyurea block that were then released to proceed through the cell cycle. The latter approach (G1/S synchronization) is critical for rigorously maintaining cell synchrony for unambiguous analysis of gene regulation in later stages of the cell cycle. Analysis of these samples identified seven distinct clusters of genes that exhibit unique patterns of expression. Genes tend to cluster within these groups based on common function and the time during the cell cycle that the activity is required. Placed in this context, the analysis of genes induced by E2F proteins identified genes or expressed sequence tags not previously described as regulated by E2F proteins; surprisingly, many of these encode proteins known to function during mitosis. A comparison of the E2F-induced genes with the patterns of cell growth-regulated gene expression revealed that virtually all of the E2F-induced genes are found in only two of the cell cycle clusters; one group was regulated at G1/S, and the second group, which included the mitotic activities, was regulated at G2. The activation of the G2 genes suggests a broader role for E2F in the control of both DNA replication and mitotic activities.

Inducing Yeast Cell Synchrony: Dilution of Stationary-Phase Cultures

2006 ◽  
Vol 2006 (1) ◽  
pp. pdb.prot4176
Author(s):  
David C. Amberg ◽  
Daniel J. Burke ◽  
Jeffrey N. Strathern
Keyword(s):  
Stationary Phase ◽  
Yeast Cell ◽  
Cell Synchrony

Noninvasive, Real-Time Method for the Examination of Thymidine Uptake Events—Application of the Method to V-79 Cell Synchrony Studies

1997 ◽  
Vol 248 (2) ◽  
pp. 251-257 ◽  
Author(s):  
Robert Graves ◽  
Rhian Davies ◽  
Gerard Brophy ◽  
Gerry O'Beirne ◽  
Neil Cook
Keyword(s):  
Real Time ◽  
Thymidine Uptake ◽  
Cell Synchrony

The zebrafish midblastula transition

Development ◽  
1993 ◽  
Vol 119 (2) ◽  
pp. 447-456 ◽  
Author(s):  
D.A. Kane ◽  
C.B. Kimmel
Keyword(s):  
Cell Cycle ◽  
Cell Motility ◽  
Cycle Length ◽  
Cell Cycle Regulation ◽  
Midblastula Transition ◽  
Timing Mechanism ◽  
Cell Synchrony ◽  
Activation Of Transcription ◽  
Blastomere Volume ◽  
Cycle Regulation

The zebrafish midblastula transition (MBT) begins at cycle 10. It is characterized by cell cycle lengthening, loss of cell synchrony, activation of transcription and appearance of cell motility. Superceding a 15 minute oscillator that controls the first nine cycles, the nucleocytoplasmic ratio appears to govern the MBT. This timing mechanism operates cell autonomously: clones of labeled cells initiate cell cycle lengthening independently of neighbors but dependent on immediate lineal ancestors. Unequal divisions, when they occur, produce asymmetric cell cycle lengthening based on the volume of each daughter. During the several cycles after the MBT begins, cycle length is correlated with the reciprocal of the blastomere volume, suggesting a continuation of cell cycle regulation by the nucleocytoplasmic ratio during an interval that we term the ‘MBT period’.

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