scholarly journals A Method for Analyzing Protein–Protein Interactions in the Plasma Membrane of Live B Cells by Fluorescence Resonance Energy Transfer Imaging as Acquired by Total Internal Reflection Fluorescence Microscopy

2009 ◽  
pp. 159-183 ◽  
Author(s):  
Hae Won Sohn ◽  
Pavel Tolar ◽  
Joseph Brzostowski ◽  
Susan K. Pierce
Keyword(s):  
Plasma Membrane ◽  
Energy Transfer ◽  
B Cells ◽  
Fluorescence Microscopy ◽  
Fluorescence Resonance Energy Transfer ◽  
Protein Interactions ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Internal Reflection ◽  
Protein Protein Interactions

scholarly journals A Novel Fluorescence Resonance Energy Transfer Assay Demonstrates that the Human Immunodeficiency Virus Type 1 Pr55Gag I Domain Mediates Gag-Gag Interactions

2004 ◽  
Vol 78 (3) ◽  
pp. 1230-1242 ◽  
Author(s):  
Aaron Derdowski ◽  
Lingmei Ding ◽  
Paul Spearman
Keyword(s):  
Human Immunodeficiency Virus ◽  
Plasma Membrane ◽  
Energy Transfer ◽  
Protein Interactions ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Protein Protein Interactions ◽  
Immunodeficiency Virus ◽  
Hiv 1

ABSTRACT Human immunodeficiency virus type 1 (HIV-1) assembly takes place at the plasma membrane of cells and is directed by the Pr55Gag polyprotein (Gag). One of the essential steps in the assembly process is the multimerization of Gag. We have developed a novel fluorescence resonance energy transfer (FRET) assay for the detection of protein-protein interactions between Gag molecules. We demonstrate that Gag multimerization takes place primarily on cellular membranes, with the majority of these interactions occurring on the plasma membrane. However, distinct sites of Gag-Gag interaction are also present at punctate intracellular locations. The I domain is a functional assembly domain within the nucleocapsid region of Gag that affects particle density, the subcellular localization of Gag, and the formation of detergent-resistant Gag protein complexes. Results from this study provide evidence that the I domain mediates Gag-Gag interactions. Using Gag-fluorescent protein fusion constructs that were previously shown to define the minimal I domain within HIV-1 Pr55Gag, we show by FRET techniques that protein-protein interactions are greatly diminished when Gag proteins lacking the I domain are expressed. Gag-Tsg101 interactions are also seen in living cells and result in a shift of Tsg101 to the plasma membrane. The results within this study provide direct evidence that the I domain mediates protein-protein interactions between Gag molecules. Furthermore, this study establishes FRET as a powerful tool for the detection of protein-protein interactions involved in retrovirus assembly.

scholarly journals Measuring cooperative Rev protein-protein interactions on Rev responsive RNA by fluorescence resonance energy transfer

RNA Biology ◽  
2011 ◽  
Vol 8 (2) ◽  
pp. 316-324 ◽  
Author(s):  
Thomas Vercruysse ◽  
Sonalika Pawar ◽  
Wim De Borggraeve ◽  
Els Pardon ◽  
George N. Pavlakis ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Fluorescence Resonance Energy Transfer ◽  
Protein Interactions ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Protein Protein Interactions ◽  
Fluorescence Resonance ◽  
Rev Protein

Fluorescence resonance energy transfer-based technologies in the study of protein–protein interactions at the cell surface

Methods ◽  
2012 ◽  
Vol 57 (4) ◽  
pp. 467-472 ◽  
Author(s):  
Víctor Fernández-Dueñas ◽  
Javier Llorente ◽  
Jorge Gandía ◽  
Dasiel O. Borroto-Escuela ◽  
Luigi F. Agnati ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Cell Surface ◽  
Fluorescence Resonance Energy Transfer ◽  
Protein Interactions ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Protein Protein Interactions ◽  
Fluorescence Resonance
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