Quantitation of 25-OH-Vitamin D (25OHD) Using Liquid Tandem Mass Spectrometry (LC-MS-MS)

2009 ◽  
pp. 509-517 ◽  
Author(s):  
Ravinder J. Singh
Keyword(s):  
Mass Spectrometry ◽  
Vitamin D ◽  
Tandem Mass Spectrometry ◽  
Tandem Mass ◽  
25 Oh Vitamin D

Comparison of Automated 25-OH Vitamin D Immunoassays with Liquid Chromatography Isotope Dilution Tandem Mass Spectrometry

2013 ◽  
Vol 59 (01+02/2013) ◽  
Author(s):  
Marja-Kaisa Koivula ◽  
Ursula Turpeinen ◽  
Pivi Laitinen ◽  
Juha Risteli
Keyword(s):  
Mass Spectrometry ◽  
Vitamin D ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Isotope Dilution ◽  
Tandem Mass ◽  
25 Oh Vitamin D

Performance characteristics of the VIDAS® 25-OH Vitamin D Total assay – comparison with four immunoassays and two liquid chromatography-tandem mass spectrometry methods in a multicentric study

2016 ◽  
Vol 54 (1) ◽  
Author(s):  
Emmanuel Moreau ◽  
Silvia Bächer ◽  
Sophie Mery ◽  
Caroline Le Goff ◽  
Nadia Piga ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Vitamin D ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Clinical Performance ◽  
Tandem Mass ◽  
Multicentric Study ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass ◽  
25 Oh Vitamin D

AbstractThe study was conducted to evaluate the analytical and clinical performance of the VIDASVIDASThe VIDASThe VIDAS

Vitamin D analyses in veterinary feeds by gas chromatography-tandem mass spectrometry

2021 ◽  
pp. 146906672110002
Author(s):  
Andreas Lehner ◽  
Margaret Johnson ◽  
Alan Zimmerman ◽  
Justin Zyskowski ◽  
John Buchweitz
Keyword(s):  
Mass Spectrometry ◽  
Vitamin D ◽  
Gas Chromatography ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Vitamin D3 ◽  
Mass Spectrometric ◽  
Chromatographic Behavior ◽  
Tandem Mass ◽  
Chromatography Tandem Mass Spectrometry

This report examines the feasibility of determination of Vitamin D3, D2 and their 25-hydroxy metabolites utilizing Gas Chromatography Tandem Mass Spectrometry (GC/MS/MS) as a potential alternative to popular Liquid Chromatography Tandem Mass Spectrometric (LC/MS/MS) methodologies. The GC/MS/MS approach was found to operate reasonably well despite long-standing concerns that gas-liquid chromatography of vitamin D compounds invoke thermal rearrangements owing to the relatively high inlet and capillary column temperatures used. The workup procedure involved incubation of feed samples with concentrated potassium hydroxide for overnight fat saponification, extraction of D Vitamins in n-hexane and reaction with N,O-bis(trimethylsilyl)trifluoroacetamide at 70 °C for 30 mins. In addition to parent compounds, small amounts of pyro-, isopyro-, and iso-vitamin D and isotachysterol3 variants were obtained from each Vitamin D-related compound upon extraction and GC/MS/MS analysis. Mass spectral and chromatographic behavior of these compounds are herein described and interpreted. Multiple Reaction Monitoring settings on GC/MS/MS included m/z 456→351 for Vitamin D3 and m/z 486→363 for Vitamin D2. Trimethylsilylation enabled single predominant peaks for Vitamins D3 and D2, and sample workup in the presence of deuterated Vitamin D analogs enabled accurate and precise sensitivity to 1 ppb (ng/g) in feeds. The method could be extended with reasonable accuracy to 25-hydroxy (25OH) compounds, but accuracies would be significantly improved by inclusion of respective 25OH-specific deuterated internal standards. The method was applied to 27 submissions of suspect dog foods of which 22% were discovered elevated and 44% were discovered to contain toxic levels of Vitamin D3. The described method was thus discovered to provide a suitable mass spectrometric approach for Vitamin D, proving itself here specifically of value in detection of ergocalciferol and cholecalciferol in animal feeds. The specificity and sensitivity of the tandem quadrupole approach can enable suitable applicability to serum determination if desired.

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