Microbial Expression of Alkaloid Biosynthetic Enzymes for Characterization of Their Properties

2010 ◽  
pp. 111-120 ◽  
Author(s):  
Hiromichi Minami ◽  
Nobuhiro Ikezawa ◽  
Fumihiko Sato
Keyword(s):  
Biosynthetic Enzymes

Electron microscope studies of the isolated dna fragments containing biotin genes of escherichia coli k12

1978 ◽  
Vol 36 (2) ◽  
pp. 198-199
Author(s):  
C.K. DasGupta ◽  
H. Samanta ◽  
A. Guha
Keyword(s):  
Gene Expression ◽  
Escherichia Coli ◽  
Electron Microscope ◽  
Linkage Map ◽  
Molecular Level ◽  
Present Report ◽  
Biosynthetic Enzymes ◽  
Electron Microscopic ◽  
Phage Dna

The genes coding for the biotin biosynthetic enzymes, bioABFCD are located at 17 min of the linkage map of EL coli K12 (Bachman, et al. Bact. Rev. 40: 116, 1976). It was shown that the bioA gene is oriented anticlockwise and the bioBFCD genes are oriented clockwise in the EL coli map, with the bio regulator region lying in between them (Guha et al. J. Mol. Biol. 56: 53, 1971). The study of the control elements of this divergently oriented operon at the molecular level is important for understanding the various intriguing mechanisms of gene expression in bacteria. In the present work, the electron microscopic characterization of the entire biotin genes and its regulator segment isolated after cleaving a biotin transducing phage DNA by restriction endonucleases, is described. The isolated biotin DNA fratments were subsequently recombined with the colEI DNA to isolate a colEl-biotin DNA hybrid. The present report also includes a portion of the study of this cloned plasmid chimera in a biotin auxotroph.

Cloning and Characterization of Genes Encoding Tetrahydrobiopterin Biosynthetic Enzymes

1993 ◽  
pp. 139-145
Author(s):  
Robert A. Levine ◽  
J. Christopher States ◽  
Panagiotis Z. Anastasiadis ◽  
Donald M. Kuhn
Keyword(s):  
Biosynthetic Enzymes ◽  
Genes Encoding

Characterization of Biotin Biosynthetic Enzymes ofBacillus sphaericus: a Dethiobiotin producing Bacterium

1981 ◽  
Vol 45 (9) ◽  
pp. 1983-1989
Author(s):  
Yoshikazu Izumi ◽  
Yoshiaki Kano ◽  
Kenji Inagaki ◽  
Naoki Kawase ◽  
Yoshiki Tani ◽  
...  
Keyword(s):  
Biosynthetic Enzymes

scholarly journals Characterization of Ferrochelatase (hemH) Mutations in Haemophilus influenzae

2000 ◽  
Vol 68 (5) ◽  
pp. 3007-3009 ◽  
Author(s):  
Stefan Schlör ◽  
Mark Herbert ◽  
Michaela Rodenburg ◽  
Julia Blass ◽  
Joachim Reidl
Keyword(s):  
Haemophilus Influenzae ◽  
Homologous Gene ◽  
Biosynthetic Enzymes ◽  
Growth Physiology ◽  
Laboratory Conditions

ABSTRACT Haemophilus influenzae lacks most of the biosynthetic enzymes for hemin synthesis. However, the organism has retained ferrochelatase activity, which we identified to be encoded by ahemH-homologous gene. In this report we characterize the growth physiology conferred by hemH mutations under infection and laboratory conditions.

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