scholarly journals Characterization of Ferrochelatase (hemH) Mutations in Haemophilus influenzae

2000 ◽  
Vol 68 (5) ◽  
pp. 3007-3009 ◽  
Author(s):  
Stefan Schlör ◽  
Mark Herbert ◽  
Michaela Rodenburg ◽  
Julia Blass ◽  
Joachim Reidl
Keyword(s):  
Haemophilus Influenzae ◽  
Homologous Gene ◽  
Biosynthetic Enzymes ◽  
Growth Physiology ◽  
Laboratory Conditions

ABSTRACT Haemophilus influenzae lacks most of the biosynthetic enzymes for hemin synthesis. However, the organism has retained ferrochelatase activity, which we identified to be encoded by ahemH-homologous gene. In this report we characterize the growth physiology conferred by hemH mutations under infection and laboratory conditions.

scholarly journals Development and Characterization of Wheat-Agropyron cristatum Introgression Lines Induced by Gametocidal Genes and Wheat ph1b Mutant

Agronomy ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 277
Author(s):  
Alejandro Copete-Parada ◽  
Carmen Palomino ◽  
Adoración Cabrera
Keyword(s):  
Genetic Resources ◽  
Agropyron Cristatum ◽  
Homologous Gene ◽  
Aegilops Cylindrica ◽  
Induction Frequency ◽  
Structural Aberrations ◽  
Translocation Lines ◽  
New Germplasm

The P genome of Agropyron cristatum Gaertn. contains many desirable genes that can be utilized as genetic resources to improve wheat. In this research, we used both the gametocidal chromosome 2Cc and the pairing homologous gene (Ph1b) mutant to induce structural aberrations and translocations between wheat and the 4P, 5P, and 6P genome chromosomes. By using the two approaches, a total of 19 wheat-A. cristatum translocations have been identified, in which 13 were induced by the Triticum aestivum cv. Chinese Spring (CS) ph1b mutant (CS ph1b) and six were induced by gametocidal chromosome 2Cc from Aegilops cylindrica Host. The wheat-4P, -5P and -6P A. cristatum translocations were characterized by in situ hybridization and by a set of conserved orthologous set (COS) molecular markers. The aberrations included centromeric translocations, terminal translocations, dicentric translocations, and deletions. The average induction frequency of chromosome structural aberrations was 10.9% using gametocidal 2Cc chromosome and 8.8% using ph1b mutant. The highest frequency obtained was for chromosome 4P using both approaches. All the wheat-A. cristatum translocation lines obtained were valuable for identifying A. cristatum chromosome 4P, 5P, and 6P related genes. In addition, these lines provided genetic resources and new germplasm accessions for the genetic improvement of wheat.

scholarly journals Characterization of the acoustic activity of Perna perna (bivalve mollusc) under laboratory conditions

2019 ◽  
Author(s):  
Ubirajara Gonçalves de Melo Júnior ◽  
Fábio Contrera Xavier ◽  
Daniel Campbell ◽  
Nilce Silveira ◽  
Leonardo Versiani ◽  
...  
Keyword(s):  
Bivalve Mollusc ◽  
Perna Perna ◽  
Acoustic Activity ◽  
Laboratory Conditions

Functional characterization of the Haemophilus influenzae 4.5S RNA

1998 ◽  
Vol 44 (1) ◽  
pp. 91-94
Author(s):  
G Scott Jenkins ◽  
Mark S Chandler ◽  
Pamela S Fink
Keyword(s):  
Haemophilus Influenzae ◽  
Functional Characterization ◽  
Coli Strain ◽  
Northern Analysis ◽  
Gene Encoding ◽  
E Coli ◽  
Functional Homolog ◽  
Polymerase Chain ◽  
4.5S Rna

The putative 4.5S RNA of Haemophilus influenzae was identified in the genome by computer analysis, amplified by the polymerase chain reaction, and cloned. We have determined that this putative 4.5S RNA will complement an Escherichia coli strain conditionally defective in 4.5S RNA production. The predicted secondary structures of the molecules were quite similar, but Northern analysis showed that the H. influenzae RNA was slightly larger than the E. coli RNA. The H. influenzae gene encoding this RNA is the functional homolog of the ffs gene in E. coli. Key words: ffs gene, complementation studies, small RNA, prokaryotic genetics.

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