Introduction to Part II: Natural Photosensitive Proteins

2011 ◽  
pp. 95-97
Author(s):  
James J. Chambers ◽  
Richard H. Kramer
Keyword(s):  
Photosensitive Proteins

Conformational changes of an oriented film of photosensitive proteins observed by polarized ATR infrared spectroscopy

2021 ◽  
Author(s):  
Maria Eleonora Temperini ◽  
Raffaella Polito ◽  
Antonia Intze ◽  
Leonetta Baldassarre ◽  
Valeria Giliberti ◽  
...  
Keyword(s):  
Infrared Spectroscopy ◽  
Conformational Changes ◽  
Oriented Film ◽  
Photosensitive Proteins

Simple and efficient system for photoconverting light-sensitive proteins in serial crystallography experiments

2017 ◽  
Vol 50 (3) ◽  
pp. 932-939 ◽  
Author(s):  
Giorgio Schirò ◽  
Joyce Woodhouse ◽  
Martin Weik ◽  
Ilme Schlichting ◽  
Robert L. Shoeman
Keyword(s):  
Structural Changes ◽  
Fluorescent Protein ◽  
Photophysical Properties ◽  
Super Resolution ◽  
High Viscosity ◽  
Optical Pump ◽  
Time Resolved ◽  
Efficient System ◽  
X Ray ◽  
Photosensitive Proteins

Proteins that change their structure in response to light absorption regulate many functional processes in living cells. Moreover, biotechnological approaches like optogenetics and super-resolution fluorescence microscopy recently triggered the generation of new genetically modified photosensitive proteins. Light-induced structural changes in photosensitive proteins can be studied by time-resolved serial femtosecond crystallography (SFX), an X-ray diffraction technique that allows the determination of macromolecular structures at X-ray free-electron lasers from a large number of nano- to micro-sized crystals. This article describes a simple and efficient system for converting photosensitive proteins into light-induced semi-stationary states by inline laser illumination prior to sample injection with a gas-focused liquid jet and subsequent optical pump–X-ray probe exposure. The simple setup of this device makes it suitable for integration into other liquid injectors (like electro-spinning and electro-kinetic injectors) and potentially also in high-viscosity extruders, provided that embedding microcrystals in viscous media does not alter protein photophysical properties. The functioning of the device is demonstrated with an example of a photoswitchable fluorescent protein pre-illuminated (photoactivated) for time-resolved SFX experiments. The device can be easily adapted for the conversion in time-resolved SFX experiments of other microcrystalline proteins, such as photosystems, phytochromes and rhodopsins.

Langmuir-Blodgett films of photosensitive proteins

1996 ◽  
Vol 33 (3) ◽  
pp. 191-200 ◽  
Author(s):  
Isidoro Mario Pepe ◽  
Claudio Nicolini
Keyword(s):  
Langmuir Blodgett ◽  
Langmuir Blodgett Films ◽  
Photosensitive Proteins

Investigations of Photosensitive Proteins by Serial Crystallography

2018 ◽  
Vol 83 (S1) ◽  
pp. S163-S175 ◽  
Author(s):  
G. K. Selikhanov ◽  
M. S. Fando ◽  
M. V. Dontsova ◽  
A. G. Gabdulkhakov
Keyword(s):  
Serial Crystallography ◽  
Photosensitive Proteins
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