Challenges in the DNA Barcoding of Plant Material

2012 ◽  
pp. 23-33 ◽  
Author(s):  
Robyn S. Cowan ◽  
Michael F. Fay
Keyword(s):  
Dna Barcoding ◽  
Plant Material

scholarly journals Unidentifiable by morphology: DNA barcoding of plant material in local markets in Iran

PLoS ONE ◽  
2017 ◽  
Vol 12 (4) ◽  
pp. e0175722 ◽  
Author(s):  
Abdolbaset Ghorbani ◽  
Yousef Saeedi ◽  
Hugo J. de Boer
Keyword(s):  
Dna Barcoding ◽  
Plant Material ◽  
Local Markets

scholarly journals Forensic botany and forensic chemistry working together: application of plant DNA barcoding as a complement to forensic chemistry—a case study in Brazil

Genome ◽  
2019 ◽  
Vol 62 (1) ◽  
pp. 11-18 ◽  
Author(s):  
Renato T.F. Paranaiba ◽  
Carlos B.V. Carvalho ◽  
Jorge M. Freitas ◽  
Levy H. Fassio ◽  
Élvio D. Botelho ◽  
...  
Keyword(s):  
Dna Barcoding ◽  
Plant Species ◽  
Plant Material ◽  
Forensic Analysis ◽  
Forensic Chemistry ◽  
Artemisia Absinthium ◽  
Genetic Sequencing ◽  
Plant Dna ◽  
Plant Matter ◽  
Working Together

Recently, Brazilian Federal Police used forensic chemistry and forensic botany techniques on a case. Two packets containing fragmented plant matter were seized and sent for forensic analysis. Forensic chemistry, the gold standard for evaluating plant material suspected to contain illicit substances, did not find illicit materials. Gas chromatography coupled mass spectrometry (GC-MS) identified thujone in the botanical material. Thujone is a chemical compound naturally found in many plant species, notably Artemisia absinthium. Because doubt remained, we next used plant DNA barcoding methods. Total DNA from plant tissue fragments was extracted and five different DNA regions were amplified, sequenced, and analyzed using plant DNA barcoding methods. Genetic analysis yielded 30 good quality sequences representing five taxa. Most specimens were identified as A. absinthium. Few studies focus on practical forensic applications of plant DNA barcoding methods using a case solved in a forensic laboratory with its difficulties and limitations. To the best of our knowledge, this is the first study to report an effective joint effort of forensic chemistry and botany techniques to assess plant material in Brazil. The availability of a new technical approach for the genetic sequencing of plant species will enhance many forensic investigations and inspire similar initiatives.

scholarly journals DNA Barcoding in Selected Species and Subspecies of Rye (Secale) Using Three Chloroplast Loci (matK, rbcL, trnH-psbA)

2018 ◽  
Vol 47 (1) ◽  
pp. 54-62 ◽  
Author(s):  
Lidia SKUZA ◽  
Izabela SZUĆKO ◽  
Ewa FILIP ◽  
Anastazja ADAMCZYK
Keyword(s):  
Chloroplast Dna ◽  
Dna Barcoding ◽  
Plant Species ◽  
Plant Material ◽  
High Variability ◽  
Universal Primers ◽  
Neighbor Joining ◽  
Cultivated Species ◽  
Chloroplast Dna Regions

DNA barcoding is a relatively new method of identifying plant species using short sequences of chloroplast DNA. Although there is a large number of studies using barcoding on various plant species, there are no such studies in the genus Secale. In this study the plant material consisted of 10 cultivated and non-cultivated species and subspecies of rye genus. Three chloroplast DNA regions (rbcL, matK, trnH-psbA) were tested for their suitability as DNA barcoding regions. Universal primers were used, and sequenced products were analyzed using Neighbor Joining and the Maximum Likelihood in the MEGA 7.1 program. We did not observe high variability in nucleotide sequences within the matK and rbcL regions. Only 2.2% of the sequences showed polymorphism in the rbcL region, while 6.5% in the matK region. The most variable trnH-psbA (15.6%) intergenic region was the most useful for rye barcoding. Individual application of the studied regions did not provide the expected results. None of the regions used in the study allowed the division of rye species and subspecies according to the adopted classification of the genus Secale. The results confirm that the use of matK and rbcL is insufficient for DNA barcoding in rye species, and better discrimination within the genus Secale can be obtained only in combination with the non-coding trnH-psbA sequence. Our results also indicate the necessity of using a different region. All of the new sequences have been deposited in Genbank.

DNA barcoding of medicinal plant material for identification

2014 ◽  
Vol 25 ◽  
pp. 103-110 ◽  
Author(s):  
Natascha Techen ◽  
Iffat Parveen ◽  
Zhiqiang Pan ◽  
Ikhlas A Khan
Keyword(s):  
Medicinal Plant ◽  
Dna Barcoding ◽  
Plant Material ◽  
Medicinal Plant Material

DNA barcoding for identification of species in mushrooms: A component of product certification

Planta Medica ◽  
2014 ◽  
Vol 80 (10) ◽  
Author(s):  
HA Raja ◽  
TR Baker ◽  
JG Little ◽  
NH Oberlies
Keyword(s):  
Dna Barcoding ◽  
Product Certification ◽  
Identification Of Species

BIOEFFICACY OF PLANT EXTRACTS AGAINST MUSTARD APHID AND THEIR NATURAL ENEMIES

2019 ◽  
Vol 25 (1) ◽  
Author(s):  
NARENDRA SINGH ◽  
N. S. BHADAURIA ◽  
PRADYUMN SINGH
Keyword(s):  
Plant Extracts ◽  
Natural Enemies ◽  
Untreated Control ◽  
Plant Material ◽  
Aphid Population ◽  
College Of Agriculture ◽  
Mustard Aphid ◽  
Plant Materials

The Bio-efficacy of eleven plant extracts namely viz.Neem Kernel; Rhizome of Ginger; Leaves of Datura, Gajarghas, Harsingar, Oak and Latjeera; Bulb of Garlic and Onion; Flowers of Chrysenthemum and Fruits of Chilli in the concentration of 5 percent and imidacloprid @ 40 g ai/ha was tested against mustard aphid, Lipaphiserysimi and their effect on D. rapae and Coccinellid beetle were tested in the Department of Entomology, College of Agriculture, Gwalior (M.P.). All the tested plant materials and imidacloprid @ 40 g ai/ha were effective significanty in reducing the aphid population over control.The aphid population in treated plots ranged from 7.2 to 40.0 as against 85.4 aphid/twig in untreated control. Among the plant material, three sprays of Neem Kernel were found most effective followed by three sprays of chilli fruits.All the plant extracts were found significantly safer to D. rapae and coccinellid bettle in comparision to insecticide (imidacloprid).

EVALUATION OF CLOVERS ON SANDY COASTAL SOIL

1977 ◽  
pp. 130-138 ◽  
Author(s):  
W.M. Williams ◽  
L.B. Anderson ◽  
B.M. Cooper
Keyword(s):  
New Zealand ◽  
Drought Stress ◽  
Sandy Soil ◽  
White Clover ◽  
Plant Material ◽  
Red Clover ◽  
Early Spring ◽  
Commercial Cultivars ◽  
Mediterranean Origin ◽  
Winter Annual

In evaluations of clover performances on summer-dry Himatangi sandy soil, it was found that none could match lucerne over summer. Emphasis was therefore placed on production in autumn-winter- early spring when lucerne growth was slow. Evaluations of some winter annual clover species suggested that Trifolium spumosum, T. pallidum, T. resupinatum, and T. vesiculosum would justify further investigation, along with T. subterraneum which is already used in pastures on this soil type. Among the perennial clover species, Kenya white clover (7'. semipilosum) showed outstanding recovery from drought and was the only species to produce significantly in autumn. However, it failed to grow in winter-early spring. Within red clover, materials of New Zealand x Moroccan origin substantially outproduced the commercial cultivars. Within white clover, material from Israel, Italy and Lebanon, as well as progeny of a selected New Zealand plant, showed more rapid recovery from drought stress and subsequently better winter growth than New Zealand commercial material ('Grasslands Huia'). The wider use of plant material of Mediterranean origin and of plants collected in New Zealand dryland pastures is advocated in development of clover cultivars for New Zealand dryland situations.

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