Activation Tagging

2011 ◽  
pp. 117-133 ◽  
Author(s):  
Xiaoping Gou ◽  
Jia Li
Keyword(s):  
Activation Tagging

Constructing and Phenotypic Analyzing an Activation-Tagging Arabidopsis Mutant Pool

2010 ◽  
Vol 32 (1) ◽  
pp. 53-59
Author(s):  
Yan-Long GUAN ◽  
Wan-Sha LI ◽  
Kui-De YIN ◽  
Yong-Ping YANG ◽  
Xiang-Yang HU
Keyword(s):  
Activation Tagging ◽  
Mutant Pool

Diverse developmental mutants revealed in an activation-tagged population of poplarThis article is one of a selection of papers published on the Special Issue of Poplar Research in Canada.

2007 ◽  
Vol 85 (11) ◽  
pp. 1071-1081 ◽  
Author(s):  
Edward J. Harrison ◽  
Michael Bush ◽  
Jonathan M. Plett ◽  
Daniel P. McPhee ◽  
Robin Vitez ◽  
...  
Keyword(s):  
Large Scale ◽  
Insertional Mutagenesis ◽  
Hybrid Poplar ◽  
Activation Tagging ◽  
Developmental Abnormalities ◽  
Endogenous Gene ◽  
Developmental Mutants ◽  
Poplar Trees ◽  
Wide Range ◽  
Mutant Lines

We have produced the largest population of activation-tagged poplar trees to date, approximately 1800 independent lines, and report on phenotypes of interest that have been identified in tissue culture and greenhouse conditions. Activation tagging is an insertional mutagenesis technique that results in the dominant upregulation of an endogenous gene. A large-scale Agrobacterium -mediated transformation protocol was used to transform the pSKI074 activation-tagging vector into Populus tremula × Populus alba hybrid poplar. We have screened the first 1000 lines for developmental abnormalities and have a visible mutant frequency of 2.4%, with alterations in leaf and stem structure as well as overall stature. Most of the phenotypes represent new phenotypes that have not previously been identified in poplar and, in some cases, not in any other plant either. Molecular analysis of the T-DNA inserts of a subpopulation of mutant lines reveal both single and double T-DNA inserts with double inserts more common in lines with visible phenotypes. The broad range of developmental mutants identified in this pilot screen of the population reveals that it will be a valuable resource for gene discovery in poplar. The full value of this population will only be realized as we screen these lines for a wide range of phenotypes.

scholarly journals Activation Tagging Using the En-I Maize Transposon System in Arabidopsis

2002 ◽  
Vol 129 (4) ◽  
pp. 1544-1556 ◽  
Author(s):  
Nayelli Marsch-Martinez ◽  
Raffaella Greco ◽  
Gert Van Arkel ◽  
Luis Herrera-Estrella ◽  
Andy Pereira
Keyword(s):  
Activation Tagging

Identification of Seed Dormancy Mutants by Activation Tagging

2011 ◽  
pp. 185-198 ◽  
Author(s):  
Tiehan Zhao ◽  
Ying Zeng ◽  
Allison R. Kermode
Keyword(s):  
Seed Dormancy ◽  
Activation Tagging

Activation tagging: a means of isolating genes implicated as playing a role in plant growth and development

1994 ◽  
pp. 285-292
Author(s):  
Richard Walden ◽  
Klaus Fritze ◽  
Hiroaki Hayashi ◽  
Edvins Miklashevichs ◽  
Hinrich Harling ◽  
...  
Keyword(s):  
Plant Growth ◽  
Growth And Development ◽  
Activation Tagging ◽  
Plant Growth And Development

scholarly journals Genome-Wide Investigation of 2,4-Diacetylphloroglucinol Protection Genes in Arabidopsis thaliana

2020 ◽  
Vol 33 (8) ◽  
pp. 1072-1079
Author(s):  
Dae-Han Chae ◽  
Da-Ran Kim ◽  
Gyeongjun Cho ◽  
Suhyeon Moon ◽  
Youn-Sig Kwak
Keyword(s):  
Activation Tagging ◽  
Biological Processes ◽  
Gene Ontology Analysis ◽  
Toxic Activity ◽  
Reverse Transcription Pcr ◽  
Thermal Asymmetric Interlaced Pcr ◽  
Tolerant Plants ◽  
Genome Wide ◽  
Negative Effect ◽  
High Concentrations

The compound 2,4-diacetylphloroglucinol (DAPG) is a well-known secondary metabolite produced by Pseudomonas spp. that are used as biocontrol agents. DAPG displays a remarkably broad spectrum of toxic activity against pathogens of plants. Yet high concentrations of DAPG may also have negative effect on plants, but the phytotoxicity of DAPG is not clearly understood. Here, we used genome-wide activation, tagging Arabidopsis plants as the model plant to investigate the plant response to DAPG. A total of 15 lines were selected as DAPG-tolerant plants from among 62,000 lines investigated. The DAPG-responsible genes were then identified via thermal asymmetric interlaced PCR and quantitative reverse transcription PCR, and the gene ontology analysis showed the distribution of these genes having different biological processes, cellular regulations, and molecular functional properties. Collectively, these findings suggest that plants may rely on several pathways to prevent DAPG phytotoxicity.

scholarly journals Development of an activation tagging system for maize

Plant Direct ◽  
2019 ◽  
Vol 3 (2) ◽  
pp. e00118
Author(s):  
John P. Davies ◽  
Vaka S. Reddy ◽  
Xing L. Liu ◽  
Avutu S. Reddy ◽  
William Michael Ainley ◽  
...  
Keyword(s):  
Activation Tagging ◽  
Tagging System
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