Oral and Pharyngeal Epithelial Keratinocyte Culture

2012 ◽  
pp. 67-79 ◽  
Author(s):  
Kantima Leelahavanichkul ◽  
J. Silvio Gutkind
Keyword(s):  
Keratinocyte Culture

scholarly journals Biologically relevant laminin as chemically defined and fully human platform for human epidermal keratinocyte culture

2018 ◽  
Vol 9 (1) ◽  
Author(s):  
Monica Suryana Tjin ◽  
Alvin Wen Choong Chua ◽  
Aida Moreno-Moral ◽  
Li Yen Chong ◽  
Po Yin Tang ◽  
...  
Keyword(s):  
Biologically Relevant ◽  
Epidermal Keratinocyte ◽  
Keratinocyte Culture ◽  
Human Epidermal Keratinocyte

Keratinocyte Culture

2010 ◽  
pp. 235-235
Author(s):  
RP Narayan
Keyword(s):  
Keratinocyte Culture

scholarly journals Isolation, cultivation and immunofluorescence characterization of lamellar keratinocytes from equine hoof by using explants

2019 ◽  
Vol 39 (4) ◽  
pp. 292-298
Author(s):  
João P.H. Pfeifer ◽  
Vitor H. Santos ◽  
Gustavo Rosa ◽  
Jaqueline B. Souza ◽  
Marcos Jun Watanabe ◽  
...  
Keyword(s):  
Current Knowledge ◽  
Primary Cultures ◽  
Culture Method ◽  
Ki 67 ◽  
Keratinocyte Culture ◽  
Cell Growth And Differentiation ◽  
Equine Hoof ◽  
Horse Health

ABSTRACT: The importance of the hoof to the horse health is clear, and the current knowledge regarding the cellular aspects of hoof keratinocytes is poor. Studies on equine keratinocyte culture are scarce. Developing keratinocyte cultures in vitro is a condition for studies on molecular biology, cell growth and differentiation. Some methods have already been established, such as those for skin keratinocyte culture. However, few methodologies are found for lamellar keratinocytes. The objective of this study was to standardize the equine hoof keratinocyte isolation and cultivation, and then characterize the cell immunophenotype. For this, the primary culture method used was through explants obtained from three regions of the equine hoof (medial dorsal, dorsal, and lateral dorsal). After the cell isolation and cultivation, the cell culture and its explants were stained with anti-pan cytokeratin (pan-CK) (AE1/AE3), vimentin (V9), p63 (4A4), and Ki-67 (MIB-1) antibodies. Cells were grown to third passage, were positive for pan-CK, p63 and Ki-67, and few cells had vimentin positive expression. As for the explants, the epidermal laminae were not stained for vimentin or Ki-67. However, some cells presented positive pan-CK and p63 expression. This study demonstrated the viability of lamellar explants of equine hooves as a form of isolating keratinocytes in primary cultures, as well as characterized the proliferation ability of such keratinocytes in monolayers.

scholarly journals Guanine Deaminase in Human Epidermal Keratinocytes Contributes to Skin Pigmentation

Molecules ◽  
2020 ◽  
Vol 25 (11) ◽  
pp. 2637
Author(s):  
Joon Min Jung ◽  
Tai Kyung Noh ◽  
Soo Youn Jo ◽  
Su Yeon Kim ◽  
Youngsup Song ◽  
...  
Keyword(s):  
Stem Cell Factor ◽  
Small Interfering Rna ◽  
Skin Pigmentation ◽  
Epidermal Keratinocytes ◽  
Melanin Content ◽  
Human Epidermal Keratinocytes ◽  
Guanine Deaminase ◽  
Keratinocyte Culture ◽  
Interfering Rna

Epidermal keratinocytes are considered as the most important neighboring cells that modify melanogenesis. Our previous study used microarray to show that guanine deaminase (GDA) gene expression is highly increased in melasma lesions. Hence, we investigated the role of GDA in skin pigmentation. We examined GDA expression in post-inflammatory hyperpigmentation (PIH) lesions, diagnosed as Riehl’s melanosis. We further investigated the possible role of keratinocyte-derived GDA in melanogenesis by quantitative PCR, immunofluorescence staining, small interfering RNA-based GDA knockdown, and adenovirus-mediated GDA overexpression. We found higher GDA positivity in the hyperpigmentary lesional epidermis than in the perilesional epidermis. Both UVB irradiation and stem cell factor (SCF) plus endothelin-1 (ET-1) were used, which are well-known melanogenic stimuli upregulating GDA expression in both keratinocyte culture alone and keratinocyte and melanocyte coculture. GDA knockdown downregulated melanin content, while GDA overexpression promoted melanogenesis in the coculture. When melanocytes were treated with UVB-exposed keratinocyte-conditioned media, the melanin content was increased. Also, GDA knockdown lowered SCF and ET-1 expression levels in keratinocytes. GDA in epidermal keratinocytes may promote melanogenesis by upregulating SCF and ET-1, suggesting its role in skin hyperpigmentary disorders.

Canine keratinocyte culture and use of a cultured epidermal autograft in a dog

2003 ◽  
Vol 16 (04) ◽  
pp. 255-259 ◽  
Author(s):  
M. A. Stanley ◽  
R. A. S. White ◽  
D. M. Anderson
Keyword(s):  
Clinical Case ◽  
Skin Flaps ◽  
Feeder Cells ◽  
Thickness Skin ◽  
Scald Injury ◽  
Keratinocyte Culture ◽  
Cultured Epithelial Autograft ◽  
Skin Reconstruction ◽  
Dressing Change ◽  
Yorkshire Terrier

SummaryA seven month old female Yorkshire Terrier was admitted with severe contracture and scarring of the ventral abdomen, thorax and limbs, after a scald injury. Surgical management consisted of multiple releasing and reconstruction skin flaps. Keratinocytes, harvested from a 1 cm2 full thickness skin biopsy, were cultured on a lawn of irradiated feeder cells, and used as a cultured epithelial autograft on the remaining skin deficits. Epithelialisation of the deficits was complete at the first dressing change, five days after application. This is the first report of successful culture of canine keratinocytes suitable for use in skin reconstruction in a clinical case.

Stages of cell cannibalism–entosis–in normal human keratinocyte culture

2015 ◽  
Vol 80 (11) ◽  
pp. 1469-1477 ◽  
Author(s):  
A. S. Garanina ◽  
L. A. Khashba ◽  
G. E. Onishchenko
Keyword(s):  
Human Keratinocyte ◽  
Keratinocyte Culture ◽  
Normal Human

Human keratinocyte culture from the peritonsillar mucosa

1996 ◽  
Vol 253 (4-5) ◽  
Author(s):  
P. Neugebauer ◽  
B. Bonnekoh ◽  
A. Wevers ◽  
O. Michel ◽  
G. Mahrle ◽  
...  
Keyword(s):  
Human Keratinocyte ◽  
Keratinocyte Culture
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