Biolistic Transformation of Cotton Zygotic Embryo Meristem

2012 ◽  
pp. 47-57 ◽  
Author(s):  
Kanniah Rajasekaran
Keyword(s):  
Zygotic Embryo ◽  
Biolistic Transformation

scholarly journals Plant regeneration and transformation of Trachyspermum ammi using Agrobacterium tumefaciens and zygotic embryos

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Masoumeh Nomani ◽  
Masoud Tohidfar
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Plant Regeneration ◽  
Genetic Transformation ◽  
Zygotic Embryo ◽  
Plant Biotechnology ◽  
Hplc Method ◽  
Zygotic Embryos ◽  
Naphthaleneacetic Acid ◽  
Transformed Plants ◽  
Trachyspermum Ammi

Abstract Background Trachyspermum ammi is one of the key medicinal plant species with many beneficial properties. Thymol is the most important substance in the essential oil of this plant. Thymol is a natural monoterpene phenol with high anti-microbial, anti-bacterial, and anti-oxidant properties. Thymol in the latest research has a significant impact on slowing the progression of cancer cells in human. In this research, embryos were employed as convenient explants for the fast and effectual regeneration and transformation of T. ammi. To regenerate this plant, Murashige and Skoog (MS) and Gamborg's B5 (B5) media were supplemented with diverse concentrations of plant growth regulators, such as 6-benzyladenine (BA), 1-naphthaleneacetic acid (NAA), 2,4-dichlorophenoxyacetic acid (2,4-D), and kinetin (kin). Transgenic Trachyspermum ammi plants were also obtained using Agrobacterium-mediated transformation and zygotic embryos explants. Moreover, two Agrobacterium tumefaciens strains (EHA101 and LBA4404) harboring pBI121-TPS2 were utilized for genetic transformation to Trachyspermum ammi. Results According to the obtained results, the highest plant-regeneration frequency was obtained with B5 medium supplemented with 0.5 mg/l BA and 1 mg/l NAA. The integrated gene was also approved using the PCR reaction and the Southern blot method. Results also showed that the EHA101 strain outperformed another strain in inoculation time (30 s) and co-cultivation period (1 day) (transformation efficiency 19.29%). Furthermore, HPLC method demonstrated that the transformed plants contained a higher thymol level than non-transformed plants. Conclusions In this research, a fast protocol was introduced for the regeneration and transformation of Trachyspermum ammi, using zygotic embryo explants in 25–35 days. Our findings confirmed the increase in the thymol in the aerial part of Trachyspermum ammi. We further presented an efficacious technique for enhancing thymol content in Trachyspermum ammi using Agrobacterium-mediated plant transformation system that can be beneficial in genetic transformation and other plant biotechnology techniques.

scholarly journals Transcriptomic analysis highlights epigenetic and transcriptional regulation during zygotic embryo development of Pinus pinaster

2013 ◽  
Vol 13 (1) ◽  
pp. 123 ◽  
Author(s):  
José J de Vega-Bartol ◽  
Marta Simões ◽  
W Lorenz ◽  
Andreia S Rodrigues ◽  
Rob Alba ◽  
...  
Keyword(s):  
Transcriptional Regulation ◽  
Embryo Development ◽  
Pinus Pinaster ◽  
Zygotic Embryo ◽  
Transcriptomic Analysis

Biolistic transformation, expression, and inheritance of bovine β-casein in soybean (Glycine max)

1999 ◽  
Vol 35 (4) ◽  
pp. 344-349 ◽  
Author(s):  
P. J. Maughan ◽  
R. Philip ◽  
M. -J. Cho ◽  
J. M. Widholm ◽  
L. O. Vodkin
Keyword(s):  
Glycine Max ◽  
Biolistic Transformation

scholarly journals Reproductive Characteristics of the Selected Cocoa (Theobroma cacao L.) Clones after Regenerated from the Somatic Embryogenesis Culture

2021 ◽  
Vol 18 (24) ◽  
pp. 1406
Author(s):  
Gibson Entuni ◽  
Hollena Nori ◽  
Rebicca Edward ◽  
Ahmad Kamil bin Mohammad Jaafar
Keyword(s):  
Somatic Embryogenesis ◽  
Reproductive Cycle ◽  
Embryo Culture ◽  
Zygotic Embryo ◽  
Reproductive Organ ◽  
Immature Zygotic Embryo ◽  
Reproductive Characteristics ◽  
Zygotic Embryo Culture ◽  
Typical Period ◽  
Immature Zygotic Embryo Culture

This study was conducted to evaluate the reproductive characteristics of 4 elite cocoa clones (MCBC1, PBC230, KKM22 and KKM4) propagated via somatic embryogenesis culture. From the findings, all clones have similar reproductive characteristics with clones from conventional grafted. However, only KKM4 clone from immature zygotic embryo culture produced the shortest staminode to style distance of 1.83 mm. This consequently influenced flower stability by reducing the efficiency of pollination by insects. It was found that this clone also has the highest number of flowers drop after anthesis (5 flowers) and lowest production of cherelle (5 cherelles). Further observation revealed that floral development from first bud visible (BBCH51) to flower anthesis (BBCH68) of all clones took around 31 days. These cocoa flowers which remained receptive soon after anthesis at 10 am (day-31) until the next day (day-32) suggesting 2 days’ period of receptivity. HIGHLIGHTS It is crucial to assess the presence of off-type characteristics in the reproductive organ structure such as the distance between staminode to style, period of reproductive cycle and stigmatic receptivity of cocoa clones regenerated from somatic embryogenesis The converging and parallel type of staminode to style distances are the ideal flower spatial arrangements for the optimal pollination in cocoa plant compared to splay type Only KKM4 clone propagated from immature zygotic embryo culture showed variation in the distance between staminode to style distance and this caused pollination failure by insect which then consequently caused minimum cherelle production All regenerated cocoa clones observed with typical period of the reproductive cycle and stigmatic receptivity GRAPHICAL ABSTRACT

scholarly journals EMBRIOGENESIS SOMATIK ANGGREK BULAN Phalaenopsis amabilis (L.) Bl: STRUKTUR DAN POLA PERKEMBANGAN

2007 ◽  
Vol 13 (1) ◽  
pp. 33-38
Author(s):  
Edy Setiti Wida Utami ◽  
Issirep Soemardi ◽  
Taryono Taryono ◽  
Endang Semiarti
Keyword(s):  
Somatic Embryo ◽  
Somatic Embryos ◽  
Zygotic Embryo ◽  
Leaf Explants ◽  
Gellan Gum ◽  
Development Pattern ◽  
Induction Medium ◽  
Embryo Germination ◽  
Maturation Medium ◽  
One Year

Research of the structure and development pattern of somatic embryos from callus of leaf explants moon orchid Phalaenopsis amabilis (L) Bl had been done. One year old of plantlets were used as explants sources. Basal leaf of these explants were cultured in Somatic Embryo Induction Medium (SEIM) e.i.: NP(New Phalaenopsis) medium added with 2 mg/L NAA, 1 mg/L BA, 10 g/L sucrose, and 2 g/L gellan gum. Then somatic embryos were transferred to EMM (Embryo Maturation Medium) e.i. NP medium added with 1 mg/L NAA, 1 mg/L BA, 10 g/L sucrose, and 2 g/L gellan gum. Finally, mature somatic embryo were transferred to NP medium without plant growth regulator as Embryo Germination Medium (EGM). The origin of somatic embryos initially from single cell at the pheriphery of embryogenic callus. These cells then devided in mitotic repeatedly formed globular proembryo, elongation embryo, and completed embryo. The structure and development pattern of somatic embryos as the same as with zygotic embryo.

scholarly journals Biolistic transformation of grapevine using minimal gene cassette technology

2006 ◽  
Vol 25 (8) ◽  
pp. 807-814 ◽  
Author(s):  
Jose R. Vidal ◽  
Julie R. Kikkert ◽  
Bruno D. Donzelli ◽  
Patricia G. Wallace ◽  
Bruce I. Reisch
Keyword(s):  
Gene Cassette ◽  
Biolistic Transformation
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