scholarly journals Biolistic transformation of grapevine using minimal gene cassette technology

2006 ◽  
Vol 25 (8) ◽  
pp. 807-814 ◽  
Author(s):  
Jose R. Vidal ◽  
Julie R. Kikkert ◽  
Bruno D. Donzelli ◽  
Patricia G. Wallace ◽  
Bruce I. Reisch
Keyword(s):  
Gene Cassette ◽  
Biolistic Transformation

scholarly journals Proteins encoded by the DpnI restriction gene cassette. Hyperproduction and characterization of the DpnI endonuclease.

1988 ◽  
Vol 263 (29) ◽  
pp. 14696-14702
Author(s):  
A G de la Campa ◽  
S S Springhorn ◽  
P Kale ◽  
S A Lacks
Keyword(s):  
Gene Cassette

scholarly journals The Role of Prophage for Genome Diversification within a Clonal Lineage of Lactobacillus johnsonii: Characterization of the Defective Prophage LJ771

2008 ◽  
Vol 190 (17) ◽  
pp. 5806-5813 ◽  
Author(s):  
Emmanuel Denou ◽  
Raymond David Pridmore ◽  
Marco Ventura ◽  
Anne-Cécile Pittet ◽  
Marie-Camille Zwahlen ◽  
...  
Keyword(s):  
Methionine Sulfoxide ◽  
Gene Cassette ◽  
Methionine Sulfoxide Reductase ◽  
Toxin Gene ◽  
Clonal Lineage ◽  
Lactobacillus Johnsonii ◽  
Reductase Gene ◽  
Phage Dna

ABSTRACT Two independent isolates of the gut commensal Lactobacillus johnsonii were sequenced. These isolates belonged to the same clonal lineage and differed mainly by a 40.8-kb prophage, LJ771, belonging to the Sfi11 phage lineage. LJ771 shares close DNA sequence identity with Lactobacillus gasseri prophages. LJ771 coexists as an integrated prophage and excised circular phage DNA, but phage DNA packaged into extracellular phage particles was not detected. Between the phage lysin gene and attR a likely mazE (“antitoxin”)/pemK (“toxin”) gene cassette was detected in LJ771 but not in the L. gasseri prophages. Expressed pemK could be cloned in Escherichia coli only together with the mazE gene. LJ771 was shown to be highly stable and could be cured only by coexpression of mazE from a plasmid. The prophage was integrated into the methionine sulfoxide reductase gene (msrA) and complemented the 5′ end of this gene, creating a protein with a slightly altered N-terminal sequence. The two L. johnsonii strains had identical in vitro growth and in vivo gut persistence phenotypes. Also, in an isogenic background, the presence of the prophage resulted in no growth disadvantage.

scholarly journals Use of a novel nonantibiotic triple marker gene cassette to monitor high survival of Pseudomonas fluorescens SBW25 on winter wheat in the field

2008 ◽  
Vol 63 (2) ◽  
pp. 156-168 ◽  
Author(s):  
Lotta Jäderlund ◽  
Maria Hellman ◽  
Ingvar Sundh ◽  
Mark J. Bailey ◽  
Janet K. Jansson
Keyword(s):  
Winter Wheat ◽  
Pseudomonas Fluorescens ◽  
Marker Gene ◽  
Gene Cassette ◽  
High Survival ◽  
Pseudomonas Fluorescens Sbw25

Biolistic transformation, expression, and inheritance of bovine β-casein in soybean (Glycine max)

1999 ◽  
Vol 35 (4) ◽  
pp. 344-349 ◽  
Author(s):  
P. J. Maughan ◽  
R. Philip ◽  
M. -J. Cho ◽  
J. M. Widholm ◽  
L. O. Vodkin
Keyword(s):  
Glycine Max ◽  
Biolistic Transformation

scholarly journals Dual gene expression cassette is superior than single gene cassette for enhancing sheath blight tolerance in transgenic rice

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Subhasis Karmakar ◽  
Kutubuddin A. Molla ◽  
Kaushik Das ◽  
Sailendra Nath Sarkar ◽  
Swapan K. Datta ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transgenic Rice ◽  
Single Gene ◽  
Gene Cassette ◽  
Sheath Blight ◽  
Expression Cassette ◽  
Gene Expression Cassette

scholarly journals Complete Genome Sequence of Wohlfahrtiimonas chitiniclastica Strain BM-Y, Isolated from the Pancreas of a Zebra in China

2016 ◽  
Vol 4 (3) ◽  
Author(s):  
Wei Zhou ◽  
Mu Li ◽  
Lingwei Zhu ◽  
Fuyou Hua ◽  
Xue Ji ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Gene Cassette ◽  
Whole Genome ◽  
Beta Lactamase ◽  
Extended Spectrum Beta Lactamase ◽  
Extended Spectrum ◽  
First Time

Here, a complete genome sequence of Wohlfahrtiimonas chitiniclastica strain BM-Y is presented. The whole genome is 2.18-Mb and contains a bla VEB-1 gene cassette which endows it with resistance to ceftazidime, ampicillin, tetracycline, etc. To our knowledge, this is the first time that an extended spectrum beta-lactamase (ESBL) type W. chitiniclastica strain has been found.

scholarly journals Molecular evaluation of drug resistance in clinical isolates of Salmonella enterica serovar Typhi from Pakistan

2013 ◽  
Vol 7 (12) ◽  
pp. 929-940 ◽  
Author(s):  
Amna Afzal ◽  
Yasra Sarwar ◽  
Aamir Ali ◽  
Abbas Maqbool ◽  
Muhammad Salman ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Nalidixic Acid ◽  
Salmonella Enterica ◽  
Single Gene ◽  
Mutation Screening ◽  
Gene Cassette ◽  
Drug Susceptibility ◽  
Multidrug Resistant ◽  
Fourth Generation ◽  
Salmonella Enterica Serovar Typhi

Introduction: This study aimed to determine the drug susceptibility patterns and genetic elements related to drug resistance in isolates of Salmonella enterica serovar Typhi (S. Typhi) from the Faisalabad region of Pakistan. Methodology: The drug resistance status of 80 isolates were evaluated by determining antimicrobial susceptibility, MICs, drug resistance genes involved, and the presence of integrons. Nalidixic acid resistance and reduced susceptibility to ciprofloxacin were also investigated by mutation screening of the gyrA, gyrB, parC, and parE genes. Results: Forty-seven (58.7%) isolates were multidrug resistant (MDR). Among the different resistance (R) types, the most commonly observed (13/80) was AmChStrTeSxtSmzTmp, which is the most frequent type observed in India and Pakistan. The most common drug resistant genes were blaTEM-1, cat, strA-strB, tetB, sul1, sul2, and dfrA7. Among the detected genes, only dfrA7 was found to be associated in the form of a single gene cassette within the class 1 integrons. Conclusions: MIC determination of currently used drugs revealed fourth-generation gatifloxacin as an effective drug against multidrug-resistant S. Typhi, but its clinical use is controversial. The Ser83→Phe substitution in gyrA was the predominant alteration in nalidixic acid-resistant isolates, exhibiting reduced susceptibility and increased MICs against ciprofloxacin. No mutations in gyrB, parC, or parE were detected in any isolate.

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