Identification of Thioredoxin Target Disulfides Using Isotope-Coded Affinity Tags

2013 ◽  
pp. 677-685 ◽  
Author(s):  
Per Hägglund ◽  
Jakob Bunkenborg ◽  
Kenji Maeda ◽  
Christine Finnie ◽  
Birte Svensson
Keyword(s):  
Affinity Tags

Affinity Tags for Protein Purification

2020 ◽  
Vol 21 (8) ◽  
pp. 821-830
Author(s):  
Vibhor Mishra
Keyword(s):  
Protein Purification ◽  
Protein Complexes ◽  
Affinity Purification ◽  
Protein Domain ◽  
Affinity Tag ◽  
Small Peptides ◽  
Affinity Tags ◽  
C Terminus ◽  
Solubility Enhancers ◽  
As Solubility

The affinity tags are unique proteins/peptides that are attached at the N- or C-terminus of the recombinant proteins. These tags help in protein purification. Additionally, some affinity tags also serve a dual purpose as solubility enhancers for challenging protein targets. By applying a combinatorial approach, carefully chosen affinity tags designed in tandem have proven to be very successful in the purification of single proteins or multi-protein complexes. In this mini-review, the key features of the most commonly used affinity tags are discussed. The affinity tags have been classified into two significant categories, epitope tags, and protein/domain tags. The epitope tags are generally small peptides with high affinity towards a chromatography resin. The protein/domain tags often perform double duty as solubility enhancers as well as aid in affinity purification. Finally, protease-based affinity tag removal strategies after purification are discussed.

Isotope-Coded Maleimide Affinity Tags for Proteomics Applications

2021 ◽  
Author(s):  
Adam P. Wdowiak ◽  
Marisa N. Duong ◽  
Rohan D. Joyce ◽  
Amber E. Boyatzis ◽  
Mark C. Walkey ◽  
...  
Keyword(s):  
Affinity Tags

Absolute Quantification of Specific Proteins in Complex Mixtures Using Visible Isotope-Coded Affinity Tags

2004 ◽  
Vol 76 (14) ◽  
pp. 4104-4111 ◽  
Author(s):  
Yu Lu ◽  
Patricia Bottari ◽  
Frantisek Turecek ◽  
Ruedi Aebersold ◽  
Michael H. Gelb
Keyword(s):  
Absolute Quantification ◽  
Complex Mixtures ◽  
Affinity Tags ◽  
Specific Proteins

scholarly journals Affinity-tags are removed from Cladosporium fulvum effector proteins expressed in the tomato leaf apoplast

2006 ◽  
Vol 57 (3) ◽  
pp. 599-608 ◽  
Author(s):  
H. Peter van Esse ◽  
Bart P. H. J. Thomma ◽  
John W. van 't Klooster ◽  
Pierre J. G. M. de Wit
Keyword(s):  
Tomato Leaf ◽  
Effector Proteins ◽  
Cladosporium Fulvum ◽  
Affinity Tags ◽  
Leaf Apoplast

scholarly journals Design of Novel Affinity Tags to Increase Recovery of Recombinant Proteins in Ther Soluble Forms

2010 ◽  
Vol 98 (3) ◽  
pp. 445a
Author(s):  
Nicholas D. Tingquist ◽  
Anna E. Daily ◽  
T.K.S. Kumar
Keyword(s):  
Recombinant Proteins ◽  
Affinity Tags

NMR Probing of Protein−Protein Interactions Using Reporter Ligands and Affinity Tags

2004 ◽  
Vol 126 (6) ◽  
pp. 1636-1637 ◽  
Author(s):  
Martin L. Ludwiczek ◽  
Bettina Baminger ◽  
Robert Konrat
Keyword(s):  
Protein Interactions ◽  
Protein Protein Interactions ◽  
Affinity Tags

scholarly journals Comparison of mitochondrial protein expression in wildtype and mitochondrial branched chain aminotransferase (BCATm) KO mice using isotope coded affinity tags (iTRAQ)

2007 ◽  
Vol 21 (5) ◽  
Author(s):  
Christopher J Lynch ◽  
Bruce A Stanley ◽  
Anne E Stanley ◽  
Manisha Nautiyal ◽  
Pengxiang She ◽  
...  
Keyword(s):  
Protein Expression ◽  
Mitochondrial Protein ◽  
Affinity Tags ◽  
Branched Chain ◽  
Branched Chain Aminotransferase

Synthesis Based on Affinity Separation: A New Methodology for High-Throughput Synthesis Using Affinity Tags

2004 ◽  
pp. 87-98 ◽  
Author(s):  
Koichi Fukase ◽  
San-Qi Zhang ◽  
Yoshiyuki Fukase ◽  
Naomi Umesako ◽  
Shoichi Kusumoto
Keyword(s):  
High Throughput ◽  
Affinity Separation ◽  
Affinity Tags

Overview of Affinity Tags for Protein Purification

2004 ◽  
Vol 36 (1) ◽  
Author(s):  
Michelle E. Kimple ◽  
John Sondek
Keyword(s):  
Protein Purification ◽  
Affinity Tags
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