Absolute Quantification of Specific Proteins in Complex Mixtures Using Visible Isotope-Coded Affinity Tags

2004 ◽  
Vol 76 (14) ◽  
pp. 4104-4111 ◽  
Author(s):  
Yu Lu ◽  
Patricia Bottari ◽  
Frantisek Turecek ◽  
Ruedi Aebersold ◽  
Michael H. Gelb
Keyword(s):  
Absolute Quantification ◽  
Complex Mixtures ◽  
Affinity Tags ◽  
Specific Proteins

Design and Synthesis of Visible Isotope-Coded Affinity Tags for the Absolute Quantification of Specific Proteins in Complex Mixtures

2004 ◽  
Vol 15 (2) ◽  
pp. 380-388 ◽  
Author(s):  
Patricia Bottari ◽  
Ruedi Aebersold ◽  
Frantisek Turecek ◽  
Michael H. Gelb
Keyword(s):  
Absolute Quantification ◽  
Complex Mixtures ◽  
Affinity Tags ◽  
Design And Synthesis ◽  
Specific Proteins ◽  
The Absolute

scholarly journals Absolute Quantification of Specific Proteins in Complex Mixtures Using Visible Isotope-Coded Affinity Tags

2007 ◽  
pp. 159-176
Author(s):  
Yu Lu ◽  
Patricia Bottari ◽  
Ruedi Aebersold ◽  
Frantisek Turecek ◽  
Michael H. Gelb
Keyword(s):  
Absolute Quantification ◽  
Complex Mixtures ◽  
Affinity Tags ◽  
Specific Proteins

scholarly journals A clickable glutamine (CliQ) derivative for the traceless reversible modification of peptides and proteins

2019 ◽  
Vol 55 (14) ◽  
pp. 2043-2045
Author(s):  
Samuel D. Whedon ◽  
Marissa K. Parker ◽  
Elizabeth L. Tyson ◽  
Tobias Ritterhoff ◽  
Patrick M. M. Shelton ◽  
...  
Keyword(s):  
Click Reaction ◽  
Complex Mixtures ◽  
Selective Isolation ◽  
Affinity Tags ◽  
Reversible Modification

The Cu(i)-mediated click reaction of proteins with affinity tags enables their selective isolation from complex mixtures.

Absolute Quantification of Allergens from Complex Mixtures: A New Sensitive Tool for Standardization of Allergen Extracts for Specific Immunotherapy

2011 ◽  
Vol 10 (4) ◽  
pp. 2113-2122 ◽  
Author(s):  
Ulla Seppälä ◽  
Claire Dauly ◽  
Sarah Robinson ◽  
Martin Hornshaw ◽  
Jørgen Nedergaard Larsen ◽  
...  
Keyword(s):  
Specific Immunotherapy ◽  
Absolute Quantification ◽  
Complex Mixtures ◽  
Sensitive Tool ◽  
Allergen Extracts

Changes Occur in Plasma Membrane Turnover when K562 Leukemia Cells are Induced to Differentiate

1982 ◽  
Vol 40 ◽  
pp. 286-287
Author(s):  
L. M. Marshall
Keyword(s):  
Plasma Membrane ◽  
Membrane Proteins ◽  
Intracellular Transport ◽  
Parent Cell ◽  
Membrane Turnover ◽  
Coated Pits ◽  
Surface Distribution ◽  
Specific Proteins ◽  
Erythroleukemic Cell ◽  
Specific Membrane

A human erythroleukemic cell line, metabolically blocked in a late stage of erythropoiesis, becomes capable of differentiation along the normal pathway when grown in the presence of hemin. This process is characterized by hemoglobin synthesis followed by rearrangement of the plasma membrane proteins and culminates in asymmetrical cytokinesis in the absence of nuclear division. A reticulocyte-like cell buds from the nucleus-containing parent cell after erythrocyte specific membrane proteins have been sequestered into its membrane. In this process the parent cell faces two obstacles. First, to organize its erythrocyte specific proteins at one pole of the cell for inclusion in the reticulocyte; second, to reduce or abolish membrane protein turnover since hemoglobin is virtually the only protein being synthesized at this stage. A means of achieving redistribution and cessation of turnover could involve movement of membrane proteins by a directional lipid flow. Generation of a lipid flow towards one pole and accumulation of erythrocyte-specific membrane proteins could be achieved by clathrin coated pits which are implicated in membrane endocytosis, intracellular transport and turnover. In non-differentiating cells, membrane proteins are turned over and are random in surface distribution. If, however, the erythrocyte specific proteins in differentiating cells were excluded from endocytosing coated pits, not only would their turnover cease, but they would also tend to drift towards and collect at the site of endocytosis. This hypothesis requires that different protein species are endocytosed by the coated vesicles in non-differentiating than by differentiating cells.

Analysis of 3-d molecular distribution with the digital imaging microscope

1988 ◽  
Vol 46 ◽  
pp. 40-41
Author(s):  
W.A. Carrington ◽  
F.S. Fay ◽  
K.E. Fogarty ◽  
L. Lifshitz
Keyword(s):  
Image Restoration ◽  
Digital Imaging ◽  
Fluorescent Dyes ◽  
Optical Axis ◽  
Computer Hardware ◽  
Computer Algorithms ◽  
Low Contrast ◽  
Specific Proteins ◽  
Effective Use ◽  
Single Living Cells

Advances in digital imaging microscopy and in the synthesis of fluorescent dyes allow the determination of 3D distribution of specific proteins, ions, GNA or DNA in single living cells. Effective use of this technology requires a combination of optical and computer hardware and software for image restoration, feature extraction and computer graphics.The digital imaging microscope consists of a conventional epifluorescence microscope with computer controlled focus, excitation and emission wavelength and duration of excitation. Images are recorded with a cooled (-80°C) CCD. 3D images are obtained as a series of optical sections at .25 - .5 μm intervals.A conventional microscope has substantial blurring along its optical axis. Out of focus contributions to a single optical section cause low contrast and flare; details are poorly resolved along the optical axis. We have developed new computer algorithms for reversing these distortions. These image restoration techniques and scanning confocal microscopes yield significantly better images; the results from the two are comparable.

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