Preparation of Multiple Site-Specific Mutant Proteins for NMR Studies by PCR-Directed Cell-Free Protein Synthesis

2013 ◽  
pp. 169-187
Author(s):  
Kiyoshi Ozawa ◽  
Ruhu Qi
Keyword(s):  
Protein Synthesis ◽  
Multiple Site ◽  
Nmr Studies ◽  
Site Specific ◽  
Mutant Proteins ◽  
Free Protein ◽  
Cell Free Protein Synthesis

Multiple Site-Specific Installations ofNε-Monomethyl-L-Lysine into Histone Proteins by Cell-Based and Cell-Free Protein Synthesis

ChemBioChem ◽  
2014 ◽  
Vol 15 (12) ◽  
pp. 1830-1838 ◽  
Author(s):  
Tatsuo Yanagisawa ◽  
Mihoko Takahashi ◽  
Takahito Mukai ◽  
Shin Sato ◽  
Masatoshi Wakamori ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Multiple Site ◽  
Histone Proteins ◽  
Site Specific ◽  
Free Protein ◽  
Cell Free Protein Synthesis

scholarly journals High-yield cell-free protein synthesis for site-specific incorporation of unnatural amino acids at two sites

2012 ◽  
Vol 418 (4) ◽  
pp. 652-656 ◽  
Author(s):  
Kiyoshi Ozawa ◽  
Karin V. Loscha ◽  
Kekini V. Kuppan ◽  
Choy Theng Loh ◽  
Nicholas E. Dixon ◽  
...  
Keyword(s):  
Amino Acids ◽  
Protein Synthesis ◽  
Unnatural Amino Acids ◽  
High Yield ◽  
Site Specific ◽  
Free Protein ◽  
Cell Free Protein Synthesis

scholarly journals Cell-free protein synthesis of perdeuterated proteins for NMR studies

2007 ◽  
Vol 39 (3) ◽  
pp. 229-238 ◽  
Author(s):  
Touraj Etezady-Esfarjani ◽  
Sebastian Hiller ◽  
Cristina Villalba ◽  
Kurt Wüthrich
Keyword(s):  
Protein Synthesis ◽  
Nmr Studies ◽  
Free Protein ◽  
Perdeuterated Proteins ◽  
Cell Free Protein Synthesis

Cell-free Protein Synthesis in an Autoinduction System for NMR Studies of Protein–Protein Interactions

2005 ◽  
Vol 32 (3) ◽  
pp. 235-241 ◽  
Author(s):  
Kiyoshi Ozawa ◽  
Slobodan Jergic ◽  
Jeffrey A. Crowther ◽  
Phillip R. Thompson ◽  
Gene Wijffels ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Protein Interactions ◽  
Protein Protein Interactions ◽  
Nmr Studies ◽  
Free Protein ◽  
Cell Free Protein Synthesis
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