Purification of Fungal Haustoria from Infected Plant Tissue by Flow Cytometry

2014 ◽  
pp. 103-110 ◽  
Author(s):  
Diana P. Garnica ◽  
John P. Rathjen
Keyword(s):  
Flow Cytometry ◽  
Plant Tissue ◽  
Infected Plant

Freeze-fracture, freeze-etch complementary pairs of virus-infected cells reveal value of etching even when relatively high concentrations of cryoprotectants are used

1978 ◽  
Vol 36 (2) ◽  
pp. 136-137
Author(s):  
Russell L. Steere ◽  
Eric F. Erbe
Keyword(s):  
Plant Tissue ◽  
Phosphate Buffer ◽  
Infected Plant ◽  
Freeze Fracture ◽  
Distilled Water ◽  
Virus Infected Plant ◽  
Infected Cells ◽  
High Concentrations

It has been assumed by many involved in freeze-etch or freeze-fracture studies that it would be useless to etch specimens which were cryoprotected by more than 15% glycerol. We presumed that the amount of cryoprotective material exposed at the surface would serve as a contaminating layer and prevent the visualization of fine details. Recent unexpected freeze-etch results indicated that it would be useful to compare complementary replicas in which one-half of the frozen-fractured specimen would be shadowed and replicated immediately after fracturing whereas the complement would be etched at -98°C for 1 to 10 minutes before being shadowed and replicated.Standard complementary replica holders (Steere, 1973) with hinges removed were used for this study. Specimens consisting of unfixed virus-infected plant tissue infiltrated with 0.05 M phosphate buffer or distilled water were used without cryoprotectant. Some were permitted to settle through gradients to the desired concentrations of different cryoprotectants.

Characterization and aggressiveness of take-all root rot pathogens isolated from symptomatic bermudagrass putting greens

2021 ◽  
Author(s):  
Cameron Stephens ◽  
Travis W Gannon ◽  
Marc Cubeta ◽  
Tim L. Sit ◽  
Jim Kerns
Keyword(s):  
Plant Tissue ◽  
Root Rot ◽  
Pathogen Detection ◽  
Infected Plant ◽  
Gaeumannomyces Graminis ◽  
In Planta ◽  
High Resolution Melt ◽  
Putting Greens ◽  
Ultradwarf Bermudagrass ◽  
Take All

Take-all root rot is a disease of ultradwarf bermudagrass putting greens caused by Gaeumannomyces graminis (Gg), Gaeumannomyces sp. (Gx), Gaeumannomyces graminicola (Ggram), Candidacolonium cynodontis (Cc), and Magnaporthiopsis cynodontis (Mc). Many etiological and epidemiological components of this disease remain unknown. Improving pathogen identification and our understanding of the aggressiveness of these pathogens along with growth at different temperatures will advance our knowledge of disease development to optimize management strategies. Take-all root rot pathogens were isolated from symptomatic bermudagrass root and stolon pieces from 16 different golf courses. Isolates of Gg, Gx, Ggram, Cc, and Mc were used to inoculate ‘Champion’ bermudagrass in an in planta aggressiveness assay. Each pathogen was also evaluated at 10, 15, 20, 25, 30, and 35C to determine growth temperature optima. Infected plant tissue was used to develop a real-time PCR high resolution melt assay for pathogen detection. This assay was able to differentiate each pathogen directly from infected plant tissue using a single primer pair. In general, Ggram, Gg, and Gx were the most aggressive while Cc and Mc exhibited moderate aggressiveness. Pathogens were more aggressive when incubated at 30C compared to 20C. While they grew optimally between 24.4 and 27.8C, pathogens exhibited limited growth at 35C and no growth at 10C. These data provide important information on this disease and its causal agents that may improve take-all root rot management.

Using Flow Cytometry Analysis in Plant Tissue Culture Derived Plants

2018 ◽  
pp. 317-332
Author(s):  
Rosa María Escobedo-Gracia-Medrano ◽  
Martha Josefa Burgos-Tan ◽  
José Roberto Ku-Cauich ◽  
Adriana Quiroz-Moreno
Keyword(s):  
Flow Cytometry ◽  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Flow Cytometry Analysis

Bacteriocin production by Pseudomonas syringae PsW-1 in plant tissue

1982 ◽  
Vol 28 (6) ◽  
pp. 600-604 ◽  
Author(s):  
Mary L. Smidt ◽  
Anne K. Vidaver
Keyword(s):  
Pseudomonas Syringae ◽  
Plant Tissue ◽  
Inner Core ◽  
Infected Plant ◽  
Resistant Mutant ◽  
Sensitive Strain ◽  
Bacteriocin Activity ◽  
Outer Sheath ◽  
Red Kidney Bean ◽  
20 Nm

The production and activity of syringacin W-1, a particulate bacteriocin made by Pseudomonas syringae PsW-1, was studied in plant tissue. The bacteriocin is rod shaped, approximately 20 nm wide and 75 nm long, and composed of an outer sheath and inner core. Both the producing strain, PsW-1, and a sensitive strain, 16, grew within red kidney bean stems. Strains PsW-1 and 16, or mutants derived from them, were injected into bean stems singly or in mixtures. All singly inoculated strains grew well. However, when the bacteriocin-producing strain was co-inoculated with the sensitive strain, the latter grew poorly, if at all. This was not due to competition for available nutrients, since the sensitive strain grew as well in the presence of a bacteriocin-nonproducing mutant as it did alone. Also, a bacteriocin-resistant mutant grew as well in the presence of the producing strain as it did alone. Bacteriocin activity and particles were recovered from infected plant tissue.

Electro-Extraction of Viruses from Infected Plant Tissue

1981 ◽  
Vol 11 (3) ◽  
pp. 321-338
Author(s):  
A. Poison ◽  
K. J. van der Merwe
Keyword(s):  
Plant Tissue ◽  
Infected Plant

Detection of the phytotoxin coronatine by ELISA and localization in infected plant tissue

2001 ◽  
Vol 58 (6) ◽  
pp. 247-258 ◽  
Author(s):  
Y.F. Zhao ◽  
W.T. Jones ◽  
P. Sutherland ◽  
D.A. Palmer ◽  
R.E. Mitchell ◽  
...  
Keyword(s):  
Plant Tissue ◽  
Infected Plant
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