Dynamic DNA Damage and Repair Modeling: Bridging the Gap Between Experimental Damage Readout and Model Structure

2019 ◽  
pp. 127-137 ◽  
Author(s):  
Mathias S. Weyland ◽  
Pauline Thumser-Henner ◽  
Carla Rohrer Bley ◽  
Simone Ulzega ◽  
Alke Petri-Fink ◽  
...  
Keyword(s):  
Dna Damage ◽  
Model Structure ◽  
Dna Damage And Repair

DNA Damage and Repair in the Brain After Cerebral Ischemia

2001 ◽  
Vol 1 (6) ◽  
pp. 483-495 ◽  
Author(s):  
Bentham Science Publisher Philip K. Liu
Keyword(s):  
Dna Damage ◽  
Cerebral Ischemia ◽  
Dna Damage And Repair ◽  
The Brain

scholarly journals Piperlongumine inhibits migration and proliferation of castration-resistant prostate cancer cells via triggering persistent DNA damage

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Ding-fang Zhang ◽  
Zhi-chun Yang ◽  
Jian-qiang Chen ◽  
Xiang-xiang Jin ◽  
Yin-da Qiu ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cell Proliferation ◽  
Dna Damage ◽  
Cell Adhesion ◽  
Anticancer Activity ◽  
Cancer Cells ◽  
Castration Resistant Prostate Cancer ◽  
Prostate Cancer Cells ◽  
Dna Damage And Repair ◽  
Castration Resistant

Abstract Background Metastatic castration-resistant prostate cancer (CRPC) is the leading cause of death among men diagnosed with prostate cancer. Piperlongumine (PL) is a novel potential anticancer agent that has been demonstrated to exhibit anticancer efficacy against prostate cancer cells. However, the effects of PL on DNA damage and repair against CRPC have remained unclear. The aim of this study was to further explore the anticancer activity and mechanisms of action of PL against CRPC in terms of DNA damage and repair processes. Methods The effect of PL on CRPC was evaluated by MTT assay, long-term cell proliferation, reactive oxygen species assay, western blot assay, flow cytometry assay (annexin V/PI staining), β-gal staining assay and DAPI staining assay. The capacity of PL to inhibit the invasion and migration of CRPC cells was assessed by scratch-wound assay, cell adhesion assay, transwell assay and immunofluorescence (IF) assay. The effect of PL on DNA damage and repair was determined via IF assay and comet assay. Results The results showed that PL exhibited stronger anticancer activity against CRPC compared to that of taxol, cisplatin (DDP), doxorubicin (Dox), or 5-Fluorouracil (5-FU), with fewer side effects in normal cells. Importantly, PL treatment significantly decreased cell adhesion to the extracellular matrix and inhibited the migration of CRPC cells through affecting the expression and distribution of focal adhesion kinase (FAK), leading to concentration-dependent inhibition of CRPC cell proliferation and concomitantly increased cell death. Moreover, PL treatment triggered persistent DNA damage and provoked strong DNA damage responses in CRPC cells. Conclusion Collectively, our findings demonstrate that PL potently inhibited proliferation, migration, and invasion of CRPC cells and that these potent anticancer effects were potentially achieved via triggering persistent DNA damage in CRPC cells.

In vitro studies of DNA damage and repair in spermatogenic cells from rats and humans

1998 ◽  
Vol 95 ◽  
pp. 213
Author(s):  
C. Bjørge ◽  
A.-K. Olsen ◽  
R. Wiger ◽  
G. Brunborg ◽  
K. Haug ◽  
...  
Keyword(s):  
Dna Damage ◽  
In Vitro Studies ◽  
Spermatogenic Cells ◽  
Dna Damage And Repair

DNA damage and repair capacity by comet assay in lymphocytes of white-collar active smokers and passive smokers (non- and ex-smokers) at workplace

2006 ◽  
Vol 167 (2) ◽  
pp. 131-141 ◽  
Author(s):  
Maria Enrica Fracasso ◽  
Denise Doria ◽  
Paola Franceschetti ◽  
Luigi Perbellini ◽  
Luciano Romeo
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
White Collar ◽  
Repair Capacity ◽  
Dna Damage And Repair

scholarly journals O21 - The role of DNA damage and repair in allergic airway inflammation

2014 ◽  
Vol 4 (Suppl 1) ◽  
pp. O21
Author(s):  
Tze Khee Chan ◽  
Xin Yi Loh ◽  
Daniel WS Tan ◽  
Bevin P Engelward ◽  
Fred WS Wong
Keyword(s):  
Dna Damage ◽  
Airway Inflammation ◽  
Allergic Airway Inflammation ◽  
Dna Damage And Repair

scholarly journals DNA Damage and Repair: Fruit and Vegetable Effects in a Feeding Trial

2010 ◽  
Vol 62 (3) ◽  
pp. 329-335 ◽  
Author(s):  
Jyh-Lurn Chang ◽  
Gang Chen ◽  
Cornelia M. Ulrich ◽  
Jeannette Bigler ◽  
Irena B. King ◽  
...  
Keyword(s):  
Dna Damage ◽  
Fruit And Vegetable ◽  
Feeding Trial ◽  
Dna Damage And Repair

DNA damage and repair capacity in lymphocytes from obstructive sleep apnea patients

2007 ◽  
Vol 48 (9) ◽  
pp. 722-727 ◽  
Author(s):  
Konstantina Kontogianni ◽  
Niki Messini-Nikolaki ◽  
Konstantinos Christou ◽  
Konstantinos Gourgoulianis ◽  
Smaragdi Tsilimigaki ◽  
...  
Keyword(s):  
Dna Damage ◽  
Obstructive Sleep Apnea ◽  
Sleep Apnea ◽  
Repair Capacity ◽  
Dna Damage And Repair ◽  
Obstructive Sleep

Radiation-induced DNA Damage and Repair in Lens Epithelial Cells of both Ptch1(+/–) and Ercc2(+/–) Mutated Mice

2021 ◽  
Vol 197 (1) ◽  
Author(s):  
S. G. R. Barnard ◽  
R. McCarron ◽  
M. Mancuso ◽  
I. De Stefano ◽  
S. Pazzaglia ◽  
...  
Keyword(s):  
Dna Damage ◽  
Epithelial Cells ◽  
Lens Epithelial Cells ◽  
Dna Damage And Repair ◽  
Radiation Induced
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