WaveM-CNN for Automatic Recognition of Sub-cellular Organelles

Author(s):  
Duc Hoa Tran ◽  
Michel Meunier ◽  
Farida Cheriet
Author(s):  
A.R. Beaudoin ◽  
G. Grondin ◽  
A. Lord ◽  
M. Pelletier

We have recently described the ultrastructural localization of NADPase activity in the exocrine pancreas of rat. The enzyme was found in the intermediate saccules of the Golgi apparatus, in dense bodies and lysosomes but was absent from zymogen granules. A very intense reaction was noticed in a peculiar structure which was termed “Snake-Like Tubule” (SLT). The purposes of the present study were firstly to delineate SLT distribution in the acinar cell and secondly to define any possible relationship or association with other cellular organelles.NADPase cytochemical reaction was performed on the pancreas of adult Sprague Dawley rats. Small lobules were excised and fixed for 50 min, at 4°C, in 2% glutaraldehyde buffered with 0.1M cacodylate at pH 7.2. Lobules were rinsed several times with the same buffer containing 570 sucrose and cut with a Mcllwayn tissue chopper. Sections were washed several times with buffer and incubated for 2 hr at 37°C in the following medium: 4mM NADPH; 40mM sodium acetate buffer, pH 5.0; 4mM lead acetate and 5% sucrose.


Author(s):  
M. K. Lamvik

When observing small objects such as cellular organelles by scanning electron microscopy, it is often valuable to use the techniques of transmission electron microscopy. The common practice of mounting and coating for SEM may not always be necessary. These possibilities are illustrated using vertebrate skeletal muscle myofibrils.Micrographs for this study were made using a Hitachi HFS-2 scanning electron microscope, with photographic recording usually done at 60 seconds per frame. The instrument was operated at 25 kV, with a specimen chamber vacuum usually better than 10-7 torr. Myofibrils were obtained from rabbit back muscle using the method of Zak et al. To show the component filaments of this contractile organelle, the myofibrils were partially disrupted by agitation in a relaxing medium. A brief centrifugation was done to clear the solution of most of the undisrupted myofibrils before a drop was placed on the grid. Standard 3 mm transmission electron microscope grids covered with thin carbon films were used in this study.


Informatica ◽  
2007 ◽  
Vol 18 (4) ◽  
pp. 603-614
Author(s):  
Darius Grigaitis ◽  
Vaida Bartkutė ◽  
Leonidas Sakalauskas

2020 ◽  
Vol 14 (10) ◽  
pp. 2263-2272
Author(s):  
Mingqiang Pan ◽  
Cheng Sun ◽  
Jizhu Liu ◽  
Yangjun Wang

PLoS ONE ◽  
2015 ◽  
Vol 10 (5) ◽  
pp. e0121838 ◽  
Author(s):  
Baiying Lei ◽  
Ee-Leng Tan ◽  
Siping Chen ◽  
Liu Zhuo ◽  
Shengli Li ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document