Investigating the Feasibility of Laser-Doppler Vibrometry for Vibrational Analysis of Living Mammalian Cells

Author(s):  
Sascha Schwarz ◽  
Stefanie Kiderlen ◽  
Robert Moerl ◽  
Stefanie Sudhop ◽  
Hauke Clausen-Schaumann ◽  
...  
2013 ◽  
Vol 415 ◽  
pp. 538-543
Author(s):  
Paolo Castellini ◽  
Milena Martarelli ◽  
Enrico Primo Tomasini

Laser Doppler Vibrometry (LDV) is a well established technique able to accurately measure vibration velocity of any kind of structure in remote, i.e. non-intrusive way, this allowing to overcome the problem of mass loading, typical of contact sensors as accelerometers and strain-gauges, which has strong influence in case of lightweight structures. Moreover, the possibility of driving automatically the laser beam, by means of moving mirrors controlled with galvanometer servo-actuators, permits to perform scanning measurements at different locations with high spatial resolution and reduced testing time and easily measure the operational deflection shapes (ODS) of the scanned surface. The exploitation of the moving mirrors has allowed to drive the laser beam in a continuous way making it to scan continuously over the structure surface and cover it completely. This way of operation, named Continuous Scanning LDV, permits to perform full-field measurements, the LDV output carrying simultaneously the time-and spatial-dependent information related to the structural vibration. A complementary strategy making use of the LDV coupled with moving mirrors is the so called Tracking LDV, where the laser beam is driven to follow a moving object whose trajectory must be known a priori or measured during operation (e.g. via an encoder in the case of rotating structures). In this paper some applications of the Tracking Laser Doppler Vibrometry (TLDV) and Continuous Scanning Laser Doppler Vibrometry (CSLDV) will be described they concerning, specifically modal and vibrational analysis of rotating structures.


2020 ◽  
Vol 60 (8) ◽  
pp. 1067-1078
Author(s):  
S. Schwarz ◽  
B. Hartmann ◽  
J. Sauer ◽  
R. Burgkart ◽  
S. Sudhop ◽  
...  

Abstract Background Investigating the mechanical properties of biological and biocompatible hydrogels is important in tissue engineering and biofabrication. Atomic force microscopy (AFM) and compression testing are routinely used to determine mechanical properties of tissue and tissue constructs. However, these techniques are slow and require mechanical contact with the sample, rendering in situ measurements difficult. Objective We therefore aim at a fast and contactless method for determining the mechanical properties of biological hydrogels and investigate if an optical method, like Laser-Doppler vibrometry (LDV), can accomplish this task. Methods LDV is a fast contactless method for mechanical analysis. Nonetheless, LDV setups operating in the visible range of the optical spectrum are difficult to use for transparent materials, such as biological hydrogels, because LDV relies on reflected or back-scattered light from the sample. We therefore use a near-infrared (NIR) scanning LDV to determine the vibration spectra of cylindrical gelatin discs of different gelatin concentration and compare the results to AFM data and unconfined compression testing. Results We show that the gelatin test structures can be analyzed, using a NIR LDV, and the Young’s moduli can be deduced from the resonance frequencies of the first normal (0,1) mode of these structures. As expected, the frequency of this mode increases with the square root of the Young’s modulus and the damping constant increases exponentially with gelatin concentration, which underpins the validity of our approach. Conclusions Our results demonstrate that NIR wavelengths are suitable for a fast, contactless vibrational analysis of transparent hydrogel structures.


2021 ◽  
Vol 70 ◽  
pp. 1-10
Author(s):  
Sara Casaccia ◽  
Erik J. Sirevaag ◽  
Mark G. Frank ◽  
Joseph A. O'Sullivan ◽  
Lorenzo Scalise ◽  
...  

2015 ◽  
Vol 24 (4) ◽  
pp. 1038-1042 ◽  
Author(s):  
Celso Accoto ◽  
Antonio Qualtieri ◽  
Ferruccio Pisanello ◽  
Carlo Ricciardi ◽  
Candido Fabrizio Pirri ◽  
...  

2021 ◽  
Author(s):  
Sejong Chun ◽  
Sibok Lee ◽  
Hyewon Yoon

Abstract Thermowells with helical strakes are becoming promising to prevent them from fatigue fracture by Kármán vortex street. Many studies suggest various kinds of measurement techniques, including strain rate measurement, acceleration measurement, and high-speed visualization to evaluate the role of Kármán vortex street to the flow-induced vibration. Nevertheless, use of laser Doppler vibrometry has not yet been reported in the literature. This study compared the tip deflection of a thermowell due to the flow-induced vibration by using the laser Doppler vibrometry and the strain rate measurement. The laser Doppler vibrometry could measure the tip deflection directly. On the other hand, the strain rate measurement had to convert the strain rate into the tip deflection through the Euler-Bernoulli beam theory. Measurement equivalence between the laser Doppler vibrometry and the strain rate measurement was discussed with the results of tip deflections of the thermowell.


2018 ◽  
Vol 184 ◽  
pp. 1030-1039 ◽  
Author(s):  
L. Maio ◽  
F. Ricci ◽  
V. Memmolo ◽  
E. Monaco ◽  
N.D. Boffa

2018 ◽  
Vol 319 ◽  
pp. 61-67 ◽  
Author(s):  
Silja Schmidtchen ◽  
Holger Fritze ◽  
Sean Bishop ◽  
Di Chen ◽  
Harry L. Tuller

2017 ◽  
Vol 99 ◽  
pp. 88-97 ◽  
Author(s):  
Ludovic Labelle ◽  
N.B. Roozen ◽  
Jan Vandenbroeck ◽  
Shuichi Akasaka ◽  
Christ Glorieux

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