Characterization of the Phenotypic Features, Immuno-modulatory Properties and Therapeutic Potentials of Wharton’s Jelly-Derived Mesenchymal Stromal Cells

2014 ◽  
pp. 311-334
Author(s):  
Ike dela Peña ◽  
Marina Bastawrous ◽  
Diego Lozano ◽  
Daniela Aguirre ◽  
Diana Hernandez ◽  
...  
Keyword(s):  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Wharton’S Jelly ◽  
Wharton's Jelly ◽  
Phenotypic Features

Derivation of Mesenchymal Stromal Cells from Ovine Umbilical Cord Wharton's Jelly

2021 ◽  
Vol 1 (1) ◽  
Author(s):  
Irene Carreras‐Sánchez ◽  
Alba López‐Fernández ◽  
Raquel Rojas‐Márquez ◽  
Roberto Vélez ◽  
Màrius Aguirre ◽  
...  
Keyword(s):  
Umbilical Cord ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Wharton’S Jelly ◽  
Wharton's Jelly

Mesenchymal Stromal Cells Derived from Wharton's Jelly

2010 ◽  
pp. 267-287
Author(s):  
Mark L. Weiss ◽  
Kiranbabu Seshareddy
Keyword(s):  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Wharton’S Jelly ◽  
Wharton's Jelly

Immunological impact of Wharton’s Jelly mesenchymal stromal cells and natural killer cell co-culture

2018 ◽  
Vol 447 (1-2) ◽  
pp. 111-124 ◽  
Author(s):  
Mehdi Najar ◽  
Mohammad Fayyad-Kazan ◽  
Nathalie Meuleman ◽  
Dominique Bron ◽  
Hussein Fayyad-Kazan ◽  
...  
Keyword(s):  
Natural Killer Cell ◽  
Natural Killer ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Killer Cell ◽  
Wharton’S Jelly ◽  
Wharton's Jelly

scholarly journals Effects of maternal obesity on Wharton’s Jelly mesenchymal stromal cells

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Heba Badraiq ◽  
Aleksandra Cvoro ◽  
Antonio Galleu ◽  
Marisa Simon ◽  
Cristian Miere ◽  
...  
Keyword(s):  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Maternal Obesity ◽  
Wharton’S Jelly ◽  
Wharton's Jelly

scholarly journals Osteogenic commitment of Wharton’s jelly mesenchymal stromal cells: mechanisms and implications for bioprocess development and clinical application

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Raquel Cabrera-Pérez ◽  
Marta Monguió-Tortajada ◽  
Ana Gámez-Valero ◽  
Raquel Rojas-Márquez ◽  
Francesc Enric Borràs ◽  
...  
Keyword(s):  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Proteomic Analysis ◽  
Invasive Procedure ◽  
Multipotent Mesenchymal Stromal Cells ◽  
Wharton’S Jelly ◽  
Tanshinone Iia ◽  
Osteogenic Potential ◽  
Alternative Source ◽  
Wharton's Jelly

Abstract Background Orthopaedic diseases are one of the major targets for regenerative medicine. In this context, Wharton’s jelly (WJ) is an alternative source to bone marrow (BM) for allogeneic transplantation since its isolation does not require an invasive procedure for cell collection and does not raise major ethical concerns. However, the osteogenic capacity of human WJ-derived multipotent mesenchymal stromal cells (MSC) remains unclear. Methods Here, we compared the baseline osteogenic potential of MSC from WJ and BM cell sources by cytological staining, quantitative real-time PCR and proteomic analysis, and assessed chemical and biological strategies for priming undifferentiated WJ-MSC. Concretely, different inhibitors/activators of the TGFβ1-BMP2 signalling pathway as well as the secretome of differentiating BM-MSC were tested. Results Cytochemical staining as well as gene expression and proteomic analysis revealed that osteogenic commitment was poor in WJ-MSC. However, stimulation of the BMP2 pathway with BMP2 plus tanshinone IIA and the addition of extracellular vesicles or protein-enriched preparations from differentiating BM-MSC enhanced WJ-MSC osteogenesis. Furthermore, greater outcome was obtained with the use of conditioned media from differentiating BM-MSC. Conclusions Altogether, our results point to the use of master banks of WJ-MSC as a valuable alternative to BM-MSC for orthopaedic conditions.

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