Label-Free Optical Biosensors for Monitoring Cellular Processes and Cytotoxic Agents at Interfaces Using Guided Modes and Advanced Phase-Contrast Imaging Techniques

2016 ◽  
pp. 443-468 ◽  
Author(s):  
Inna Székács ◽  
Robert Horvath ◽  
András Székács
Keyword(s):  
Phase Contrast ◽  
Imaging Techniques ◽  
Cytotoxic Agents ◽  
Phase Contrast Imaging ◽  
Optical Biosensors ◽  
Label Free ◽  
Contrast Imaging ◽  
Cellular Processes ◽  
Guided Modes ◽  
Advanced Phase

Label-free microfluidic platform for blood analysis based on phase-contrast imaging

2021 ◽  
Author(s):  
Lisa Miccio ◽  
Flora Cimmino ◽  
Ivana Kurelac ◽  
Massimiliano M. Villone ◽  
Vittorio Bianco ◽  
...  
Keyword(s):  
Phase Contrast ◽  
Blood Analysis ◽  
Phase Contrast Imaging ◽  
Label Free ◽  
Contrast Imaging ◽  
Microfluidic Platform

Design, development and first experiments on the X-ray imaging beamline at Indus-2 synchrotron source RRCAT, India

2015 ◽  
Vol 22 (6) ◽  
pp. 1531-1539 ◽  
Author(s):  
A. K. Agrawal ◽  
B. Singh ◽  
Y. S. Kashyap ◽  
M. Shukla ◽  
P. S. Sarkar ◽  
...  
Keyword(s):  
Phase Contrast ◽  
Imaging Techniques ◽  
Phase Contrast Imaging ◽  
Design Development ◽  
Contrast Imaging ◽  
Synchrotron Source ◽  
Full Field ◽  
X Ray ◽  
X Ray Imaging ◽  
Micro Tomography

A full-field hard X-ray imaging beamline (BL-4) was designed, developed, installed and commissioned recently at the Indus-2 synchrotron radiation source at RRCAT, Indore, India. The bending-magnet beamline is operated in monochromatic and white beam mode. A variety of imaging techniques are implemented such as high-resolution radiography, propagation- and analyzer-based phase contrast imaging, real-time imaging, absorption and phase contrast tomographyetc. First experiments on propagation-based phase contrast imaging and micro-tomography are reported.

scholarly journals Absolute zinc quantification at the sub-cellular level by combined use of hard X-ray fluorescence and phase contrast imaging techniques

2013 ◽  
Vol 463 ◽  
pp. 012021 ◽  
Author(s):  
Ewelina Kosior ◽  
Sylvain Bohic ◽  
Heikki Suhonen ◽  
Peter Cloetens
Keyword(s):  
Phase Contrast ◽  
Imaging Techniques ◽  
Cellular Level ◽  
Phase Contrast Imaging ◽  
Contrast Imaging ◽  
X Ray ◽  
Combined Use

scholarly journals Digital, Rapid, Accurate, and Label-Free Enumeration of Viable Microorganisms Enabled by Custom-Built On-Glass-Slide Culturing Device and Microscopic Scanning

Sensors ◽  
2018 ◽  
Vol 18 (11) ◽  
pp. 3700 ◽  
Author(s):  
Donghui Song ◽  
Haomin Liu ◽  
Qiuchen Dong ◽  
Zichao Bian ◽  
Huixiang Wu ◽  
...  
Keyword(s):  
Phase Contrast ◽  
Glass Slide ◽  
Phase Contrast Imaging ◽  
Label Free ◽  
Fluorescent Staining ◽  
Contrast Imaging ◽  
E Coli ◽  
Colony Counting ◽  
Gel Film ◽  
Rapid Formation

Accurately measuring the number of viable microorganisms plays an essential role in microbiological studies. Since the conventional agar method of enumerating visible colonies is time-consuming and not accurate, efforts have been made towards overcoming these limitations by counting the invisible micro-colonies. However, none of studies on micro-colony counting was able to save significant time or provide accurate results. Herein, we developed an on-glass-slide cell culture device that enables rapid formation of micro-colonies on a 0.38 mm-thick gel film without suffering from nutrient and oxygen deprivation during bacteria culturing. Employing a phase contrast imaging setup, we achieved rapid microscopic scanning of micro-colonies within a large sample area on the thin film without the need of fluorescent staining. Using Escherichia coli (E. coli) as a demonstration, our technique was able to shorten the culturing time to within 5 h and automatically enumerate the micro-colonies from the phase contrast images. Moreover, this method delivered more accurate counts than the conventional visible colony counting methods. Due to these advantages, this imaging-based micro-colony enumeration technique provides a new platform for the quantification of viable microorganisms.

scholarly journals Comparison of propagation- and grating-based x-ray phase-contrast imaging techniques with a liquid-metal-jet source

2014 ◽  
Author(s):  
T. Zhou ◽  
U. Lundström ◽  
Thomas Thüring ◽  
S. Rutishauser ◽  
D. H. Larsson ◽  
...  
Keyword(s):  
Liquid Metal ◽  
Phase Contrast ◽  
Imaging Techniques ◽  
Phase Contrast Imaging ◽  
Contrast Imaging ◽  
X Ray ◽  
Metal Jet

scholarly journals Advanced phase-contrast imaging using a grating interferometer

2009 ◽  
Vol 16 (4) ◽  
pp. 562-572 ◽  
Author(s):  
Samuel Alan McDonald ◽  
Federica Marone ◽  
Christoph Hintermüller ◽  
Gordan Mikuljan ◽  
Christian David ◽  
...  
Keyword(s):  
Phase Contrast ◽  
Phase Contrast Imaging ◽  
Contrast Imaging ◽  
Advanced Phase ◽  
Grating Interferometer

scholarly journals Compact laser accelerators for X-ray phase-contrast imaging

2014 ◽  
Vol 372 (2010) ◽  
pp. 20130032 ◽  
Author(s):  
Z. Najmudin ◽  
S. Kneip ◽  
M. S. Bloom ◽  
S. P. D. Mangles ◽  
O. Chekhlov ◽  
...  
Keyword(s):  
Generation X ◽  
Phase Contrast ◽  
Electron Beams ◽  
Imaging Techniques ◽  
Phase Contrast Imaging ◽  
Fourth Generation ◽  
Light Sources ◽  
Contrast Imaging ◽  
Synchrotron Source ◽  
X Ray

Advances in X-ray imaging techniques have been driven by advances in novel X-ray sources. The latest fourth-generation X-ray sources can boast large photon fluxes at unprecedented brightness. However, the large size of these facilities means that these sources are not available for everyday applications. With advances in laser plasma acceleration, electron beams can now be generated at energies comparable to those used in light sources, but in university-sized laboratories. By making use of the strong transverse focusing of plasma accelerators, bright sources of betatron radiation have been produced. Here, we demonstrate phase-contrast imaging of a biological sample for the first time by radiation generated by GeV electron beams produced by a laser accelerator. The work was performed using a greater than 300 TW laser, which allowed the energy of the synchrotron source to be extended to the 10–100 keV range.

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