Hydrogen Gas Generation from Enzymatic Hydrolysis of Pre-Treated Rice Straw by Bacteria Through Dark Fermentation

Author(s):  
R. Singh ◽  
C. Srivastava ◽  
M. Srivastava
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Chiranjeevi Thulluri ◽  
Ravi Balasubramaniam ◽  
Harshad Ravindra Velankar

AbstractCellulolytic enzymes can readily access the cellulosic component of lignocellulosic biomass after the removal of lignin during biomass pretreatment. The enzymatic hydrolysis of cellulose is necessary for generating monomeric sugars, which are then fermented into ethanol. In our study, a combination of a deep eutectic (DE) mixture (of 2-aminoethanol and tetra-n-butyl ammonium bromide) and a cyclic ether (tetrahydrofuran) was used for selective delignification of rice straw (RS) under mild conditions (100 °C). Pretreatment with DE-THF solvent system caused ~ 46% delignification whereas cellulose (~ 91%) and hemicellulose (~ 67%) recoveries remained higher. The new solvent system could be reused upto 10 subsequent cycles with the same effectivity. Interestingly, the DE-THF pretreated cellulose showed remarkable enzymatic hydrolysability, despite an increase in its crystallinity to 72.3%. Contrary to conventional pretreatments, we report for the first time that the enzymatic hydrolysis of pretreated cellulose is enhanced by the removal of lignin during DE-THF pretreatment, notwithstanding an increase in its crystallinity. The current study paves way for the development of newer strategies for biomass depolymerization with DES based solvents.


2003 ◽  
Vol 105 (1-3) ◽  
pp. 155-164 ◽  
Author(s):  
Betzabé Sulbarán-De-Ferrer ◽  
Marielena Aristiguieta ◽  
Bruce E. Dale ◽  
Alexis Ferrer ◽  
Graciela Ojeda-De-Rodriguez

2012 ◽  
Vol 123 ◽  
pp. 636-645 ◽  
Author(s):  
Kyoung-Mi Lee ◽  
Dayanand Kalyani ◽  
Manish Kumar Tiwari ◽  
Tae-Su Kim ◽  
Saurabh Sudha Dhiman ◽  
...  

Author(s):  
Jaruwan Damaurai ◽  
Thanchanok Preechakun ◽  
Marisa Raita ◽  
Verawat Champreda ◽  
Navadol Laosiripojana

1988 ◽  
Vol 26 (2) ◽  
pp. 85-95 ◽  
Author(s):  
Douglas B. Rivers ◽  
George H. Emert

Materials ◽  
2018 ◽  
Vol 11 (5) ◽  
pp. 802 ◽  
Author(s):  
Ken-Lin Chang ◽  
Xiao-Qin Wang ◽  
Ye-Ju Han ◽  
Hao Deng ◽  
Jing-yong Liu ◽  
...  

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