Effect of Solution Compositions on Optimum Redox Potential in Bioleaching of Chalcopyrite by Moderately Thermophilic Bacteria

2015 ◽  
pp. 225-227 ◽  
Author(s):  
Hongbo Zhao ◽  
Jun Wang ◽  
Wenqing Qin ◽  
Guanzhou Qiu
2015 ◽  
Vol 151 ◽  
pp. 141-150 ◽  
Author(s):  
Hongbo Zhao ◽  
Jun Wang ◽  
Congren Yang ◽  
Minghao Hu ◽  
Xiaowen Gan ◽  
...  

Proceedings ◽  
2021 ◽  
Vol 66 (1) ◽  
pp. 31
Author(s):  
Sachiko Nakamura ◽  
Norio Kurosawa

Lignocellulosic biomass comprises cellulose, hemicellulose, and lignin and is a potential source of fuels and chemicals. Although this complex biomass is persistent, it can be cooperatively decomposed by a microbial consortium in nature. In this study, a coculture of the moderately thermophilic bacteria Thermobifida fusca and Ureibacillus thermosphaericus was used for biodegradation of rice chaff. The bacterial strains were incubated in modified Brock’s basal salt medium (pH 8.0) supplemented with yeast extract and rice chaff at 50 °C for 7 days. The concentration of reducing sugars and the enzymatic activities of laccase, lignin peroxidase, cellulase, and xylanase in the supernatant of the culture medium were measured every day. The concentrations of reducing sugars in solo cultures of T. fusca and U. thermosphaericus and a mixed culture of the two strains after 7 days of incubation were 0.047, 0.040, and 0.195 mg/mL, respectively, indicating that the decomposition of rice chaff was enhanced in the coculture. Based on the results, it is thought that the lignin surrounding the cellulose was decomposed by laccase and lignin peroxidase secreted from U. thermosphaericus, resulting in cellulose and hemicellulose in the rice chaff being easily decomposed by enzymes from T. fusca.


2006 ◽  
Vol 72 (5) ◽  
pp. 3375-3382 ◽  
Author(s):  
Ebaid M. A. Ibrahim ◽  
Matthias Arensk�tter ◽  
Heinrich Luftmann ◽  
Alexander Steinb�chel

ABSTRACT The enrichment and isolation of thermophilic bacteria capable of rubber [poly(cis-1,4-isoprene)] degradation revealed eight different strains exhibiting both currently known strategies used by rubber-degrading mesophilic bacteria. Taxonomic characterization of these isolates by 16S rRNA gene sequence analysis demonstrated closest relationships to Actinomadura nitritigenes, Nocardia farcinica, and Thermomonospora curvata. While strains related to N. farcinica exhibited adhesive growth as described for mycolic acid-containing actinomycetes belonging to the genus Gordonia, strains related to A. nitritigenes and T. curvata formed translucent halos on natural rubber latex agar as described for several mycelium-forming actinomycetes. For all strains, optimum growth rates were observed at 50�C. The capability of rubber degradation was confirmed by mineralization experiments and by gel permeation chromatography (GPC). Intermediates resulting from early degradation steps were purified by preparative GPC, and their analysis by infrared spectroscopy revealed the occurrence of carbonyl carbon atoms. Staining with Schiff's reagent also revealed the presence of aldehyde groups in the intermediates. Bifunctional isoprenoid species terminated with a keto and aldehyde function were found by matrix-assisted laser desorption ionization-time-of-flight and electrospray ionization mass spectrometry analyses. Evidence was obtained that biodegradation of poly(cis-1,4-isoprene) is initiated by endocleavage, rather than by exocleavage. A gene (lcp) coding for a protein with high homology to Lcp (latex-clearing protein) from Streptomyces sp. strain K30 was identified in Nocardia farcinica E1. Streptomyces lividans TK23 expressing this Lcp homologue was able to cleave synthetic poly(cis-1,4-isoprene), confirming its involvement in initial polymer cleavage.


2015 ◽  
Vol 1130 ◽  
pp. 414-417
Author(s):  
R.E. Rivera Santillan ◽  
F. Patricio Ramirez ◽  
V.D. Aguilar Perez

The recovery of metallic aluminum from the waste generated in the production of secondary aluminum by bioleaching process using the nutrient medium MKM was investigated. The development of this project was carried out due to the need to treat waste foundry which have a major impact on the environment, using environmentally friendly techniques, such as bioleaching. At work, bioleaching of smelting slag of aluminum generated in the laboratories of the Department of Metallurgy, Faculty of Chemistry, UNAM, was reported. Tests were conducted using thermophilic microorganisms at 70°C in an orbital incubator at natural pH, obtaining metallic clean aluminum, aluminum in solution and salts solution. The pH, bacterial growth and redox potential were monitored daily. We conclude that bioleaching of aluminum slags is feasible. The lack of information about the treatment of this type of industrial waste, makes this research is pioneer in this field.


2015 ◽  
Vol 25 (8) ◽  
pp. 2725-2733 ◽  
Author(s):  
Hong-bo ZHAO ◽  
Jun WANG ◽  
Wen-qing QIN ◽  
Xi-hua ZHENG ◽  
Lang TAO ◽  
...  

2015 ◽  
Vol 65 (Pt_2) ◽  
pp. 549-555 ◽  
Author(s):  
O. L. Kovaleva ◽  
A. Yu. Merkel ◽  
A. A. Novikov ◽  
R. V. Baslerov ◽  
S. V. Toshchakov ◽  
...  

Three strains of facultatively aerobic, moderately thermophilic bacteria were isolated from terrestrial hot springs in Baikal Lake region and Kamchatka (Russia). Cells of the new isolates were cocci reproducing by binary fission. The temperature range for growth was between 20 and 56 °C and the pH range for growth from pH 4.5 to 8.5, with optimal growth at 47–50 °C and pH 7.0–7.5. The organisms were chemoheterotrophs preferring sugars and polysaccharides as growth substrates. 16S rRNA gene sequences of strains 2842, 2813 and 2918Kr were nearly identical (99.7–100 % similarity) and indicated that the strains belonged to the phylum Planctomycetes . The phylogenetically closest cultivated relatives were Algisphaera agarilytica 06SJR6-2T and Phycisphaera mikurensis FYK2301M01T with 16S rRNA gene sequence similarity values of 82.4 and 80.3 %, respectively. The novel strains differed from them by higher growth temperature, sensitivity to NaCl concentration above 3.0 % and by their cellular fatty acids profile. On the basis of phylogenetic and physiological data, strains 2842T, 2813 and 2918Kr represent a novel genus and species for which we propose the name Tepidisphaera mucosa sp. nov. The type strain is 2842T ( = VKM B-2832T = JCM 19875T). We also propose that Tepidisphaera gen. nov. is the type genus of a novel family, Tepidisphaeraceae fam. nov. and a novel order, Tepidisphaerales ord. nov.


2003 ◽  
Vol 30 (4) ◽  
pp. 225-238 ◽  
Author(s):  
M. Kolari ◽  
J. Nuutinen ◽  
F. A. Rainey ◽  
M. S. Salkinoja-Salonen

2011 ◽  
Vol 5 (2) ◽  
pp. 56-60 ◽  
Author(s):  
Yoko Kawasaki ◽  
Madoka Aoki ◽  
Yoriyasu Makino ◽  
Hiroyuki Sakai ◽  
Yuki Tsuboi ◽  
...  

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