Evolution of Molecular Investigations on Sturgeon Sex Determination and Most Recent Developments in DNA Methylation with a Focus on the Siberian Sturgeon

2017 ◽  
pp. 71-91 ◽  
Author(s):  
Rémy Simide ◽  
Sandrine Gaillard
Keyword(s):  
Dna Methylation ◽  
Sex Determination ◽  
Siberian Sturgeon ◽  
Recent Developments

DNA methylation detection: recent developments in bisulfite free electrochemical and optical approaches

The Analyst ◽  
2018 ◽  
Vol 143 (20) ◽  
pp. 4802-4818 ◽  
Author(s):  
Ripon Bhattacharjee ◽  
Sofia Moriam ◽  
Muhammad Umer ◽  
Nam-Trung Nguyen ◽  
Muhammad J. A. Shiddiky
Keyword(s):  
Dna Methylation ◽  
Detection Methods ◽  
Electrochemical Biosensors ◽  
Bisulfite Treatment ◽  
Free Dna ◽  
Recent Developments

The recent development of optical and electrochemical biosensors for bisulfite treatment free DNA methylation detection methods have been reviewed. The major challenges associated with the bisulfite treatment in DNA methylation detection and their potential solutions are also discussed.

Sex Determination and Differentiation of the Siberian Sturgeon

2017 ◽  
pp. 93-113 ◽  
Author(s):  
Denise Vizziano-Cantonnet ◽  
Santiago Di Landro ◽  
André Lasalle
Keyword(s):  
Sex Determination ◽  
Siberian Sturgeon

scholarly journals DNA Methylation Reprogramming during Sex Determination and Transition in Zebrafish

2020 ◽  
Author(s):  
Xinxin Wang ◽  
Xin Ma ◽  
Gaobo Wei ◽  
Weirui Ma ◽  
Zhen Zhang ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Sex Determination ◽  
Sexual Development ◽  
Epigenetic Modification ◽  
Sex Differentiation ◽  
Primordial Germ Cells ◽  
Regulation Of Gene Expression ◽  
Adult Zebrafish ◽  
Germline Development ◽  
Male Sex

AbstractIt is a mystery about sex determination and sexual plasticity in species without sex chromosomes. DNA methylation is a prevalent epigenetic modification in vertebrates, which has been shown to involve in the regulation of gene expression and embryo development. However, it remains unclear about how DNA methylation regulates sexual development. To elucidate it, we used zebrafish to investigate DNA methylation reprogramming during juvenile germ cell development and adult female-to-male sex transition. We revealed that primordial germ cells (PGCs) undergo significant DNA methylation reprogramming during germline development and set to an oocyte/ovary-like pattern at 9 days post fertilization (9 dpf). When blocking DNMTs activity in juveniles after 9 dpf, the zebrafish preferably develops into females. We also show that Tet3 involves in PGC development. Notably, we find that DNA methylome reprogramming during adult zebrafish sex transition is similar to the reprogramming during the sex differentiation from 9 dpf PGCs to sperm. Furthermore, inhibiting DNMTs activity can prevent the female-to-male sex transition, suggesting that methylation reprogramming is required for zebrafish sex transition. In summary, DNA methylation plays important roles in zebrafish germline development and sexual plasticity.

scholarly journals DNA methylation in germ cell tumour aetiology: current understanding and outstanding questions

Reproduction ◽  
2013 ◽  
Vol 146 (2) ◽  
pp. R49-R60 ◽  
Author(s):  
Martin Cusack ◽  
Paul Scotting
Keyword(s):  
Dna Methylation ◽  
Germ Cell ◽  
Epigenetic Modification ◽  
Germ Cell Tumour ◽  
Genome Wide ◽  
Recent Developments ◽  
Methylator Phenotype ◽  
Aberrant Dna Methylation ◽  
Promoter Region Hypermethylation

Germ cell tumours (GCTs) are a diverse group of neoplasms that can be histologically subclassified as either seminomatous or non-seminomatous. These two subtypes have distinct levels of differentiation and clinical characteristics, the non-seminomatous tumours being associated with poorer prognosis. In this article, we review how different patterns of aberrant DNA methylation relate to these subtypes. Aberrant DNA methylation is a hallmark of all human cancers, but particular subsets of cancers show unusually high frequencies of promoter region hypermethylation. Such a ‘methylator phenotype’ has been described in non-seminomatous tumours. We discuss the possible cause of distinct methylation profiles in GCTs and the potential of DNA methylation to provide new targets for therapy. We also consider how recent developments in our understanding of this epigenetic modification and the development of genome-wide technologies are shedding new light on the role of DNA methylation in cancer aetiology.

scholarly journals Daphnia as an Emerging Epigenetic Model Organism

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Kami D. M. Harris ◽  
Nicholas J. Bartlett ◽  
Vett K. Lloyd
Keyword(s):  
Heavy Metals ◽  
Dna Methylation ◽  
Sex Determination ◽  
Large Body ◽  
Model Organism ◽  
Environmental Stressors ◽  
Epigenetic Mechanisms ◽  
Transgenic Technology ◽  
Ecological Literature ◽  
Epigenetic Effects

Daphnia offer a variety of benefits for the study of epigenetics. Daphnia’s parthenogenetic life cycle allows the study of epigenetic effects in the absence of confounding genetic differences. Sex determination and sexual reproduction are epigenetically determined as are several other well-studied alternate phenotypes that arise in response to environmental stressors. Additionally, there is a large body of ecological literature available, recently complemented by the genome sequence of one species and transgenic technology. DNA methylation has been shown to be altered in response to toxicants and heavy metals, although investigation of other epigenetic mechanisms is only beginning. More thorough studies on DNA methylation as well as investigation of histone modifications and RNAi in sex determination and predator-induced defenses using this ecologically and evolutionarily important organism will contribute to our understanding of epigenetics.

scholarly journals The association of changes in DNA methylation with temperature-dependent sex determination in cucumber

2017 ◽  
Vol 68 (11) ◽  
pp. 2899-2912 ◽  
Author(s):  
Yun-Song Lai ◽  
Xiaohui Zhang ◽  
Wei Zhang ◽  
Di Shen ◽  
Haiping Wang ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Sex Determination ◽  
Temperature Dependent

scholarly journals The Role of DNA Methylation Reprogramming during Sex Determination and Transition in Zebrafish

2021 ◽  
Author(s):  
Xinxin Wang ◽  
Xin Ma ◽  
Gaobo Wei ◽  
Weirui Ma ◽  
Zhen Zhang ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Sex Determination
Sign in / Sign up

Export Citation Format

Share Document