Hairy Root Culture of Nicotiana tabacum (Tobacco) as a Platform for Gene Manipulation of Secondary Metabolism

2017 ◽  
pp. 145-163 ◽  
Author(s):  
Mahesh Kumar ◽  
Adinpunya Mitra
Keyword(s):  
Nicotiana Tabacum ◽  
Secondary Metabolism ◽  
Hairy Root ◽  
Root Culture ◽  
Hairy Root Culture ◽  
Gene Manipulation

scholarly journals Advances and Perspectives in Tissue Culture and Genetic Engineering of Cannabis

2021 ◽  
Vol 22 (11) ◽  
pp. 5671
Author(s):  
Mohsen Hesami ◽  
Austin Baiton ◽  
Milad Alizadeh ◽  
Marco Pepe ◽  
Davoud Torkamaneh ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Genetic Engineering ◽  
Suspension Culture ◽  
Secondary Metabolite ◽  
Hairy Root ◽  
Root Culture ◽  
Hairy Root Culture ◽  
Gene Transformation ◽  
Secondary Metabolite Production ◽  
Metabolite Production

For a long time, Cannabis sativa has been used for therapeutic and industrial purposes. Due to its increasing demand in medicine, recreation, and industry, there is a dire need to apply new biotechnological tools to introduce new genotypes with desirable traits and enhanced secondary metabolite production. Micropropagation, conservation, cell suspension culture, hairy root culture, polyploidy manipulation, and Agrobacterium-mediated gene transformation have been studied and used in cannabis. However, some obstacles such as the low rate of transgenic plant regeneration and low efficiency of secondary metabolite production in hairy root culture and cell suspension culture have restricted the application of these approaches in cannabis. In the current review, in vitro culture and genetic engineering methods in cannabis along with other promising techniques such as morphogenic genes, new computational approaches, clustered regularly interspaced short palindromic repeats (CRISPR), CRISPR/Cas9-equipped Agrobacterium-mediated genome editing, and hairy root culture, that can help improve gene transformation and plant regeneration, as well as enhance secondary metabolite production, have been highlighted and discussed.

A reliable and efficient protocol for induction of hairy roots in Agastache foeniculum

Biologia ◽  
2014 ◽  
Vol 69 (7) ◽  
Author(s):  
Elnaz Nourozi ◽  
Bahman Hosseini ◽  
Abbas Hassani
Keyword(s):  
Salicylic Acid ◽  
Agrobacterium Rhizogenes ◽  
Hairy Root ◽  
Hairy Roots ◽  
High Performance ◽  
Root Culture ◽  
Hairy Root Culture ◽  
Biochemical Properties ◽  
Separate Experiment ◽  
Transformed Roots

AbstractHairy root culture system is a valuable tool to study the characteristics of gene expression, gene function, root biology, biochemical properties and biosynthesis pathways of secondary metabolites. In the present study, hairy roots were established in Anise hyssop (Agastache foeniculum) via Agrobacterium rhizogenes. Three strains of Agrobacterium rhizogenes (A4, A7 and 9435), were used for induction of hairy roots in four various explants (hypocotyl, cotyledon, one-month-old leaf and five-month-old leaf) of Anise hyssop. The highest frequency of transformation was achieved using A4 strain in one-month-old leaves (51.1%). The transgenic states of hairy root lines were confirmed by PCR (Polymerase chain reaction) method. High performance liquid chromatography analysis revealed that the production of rosmarinic acid (RA) in transformed roots of A. foeniculum was almost 4-fold higher than that of the non-transformed roots. In a separate experiment, hairy roots obtained from one-month-old leaves inoculated with A4 strain, were grown in liquid medium and the effects of different concentrations of salicylic acid (0.0, 0.01, 0.1 and 1 mM) and chitosan (0, 50, 100 and 150 mg L−1) (as elicitor) and sucrose (20, 30, 40 and 50 g L−1) on the growth of hairy roots were evaluated. The results showed that, 30 g L−1 sucrose and 100 mg L−1 chitosan increased the biomass of hairy root cultures and application of salicylic acid reduced the growth of hairy roots compared with control roots.

Purification and stabilization of ricin B from tobacco hairy root culture medium by aqueous two-phase extraction

2005 ◽  
Vol 117 (1) ◽  
pp. 39-48 ◽  
Author(s):  
Chenming Zhang ◽  
Fabricio Medina-Bolivar ◽  
Scott Buswell ◽  
Carole L. Cramer
Keyword(s):  
Hairy Root ◽  
Culture Medium ◽  
Root Culture ◽  
Hairy Root Culture ◽  
Phase Extraction ◽  
Two Phase ◽  
Aqueous Two Phase Extraction

Establishment of hairy root culture of Rubia yunnanensis Diels: production of Rubiaceae-type cyclopeptides and quinones

2021 ◽  
Author(s):  
Yuanyuan Miao ◽  
Yanyun Hu ◽  
Shanyong Yi ◽  
Xuejia Zhang ◽  
Ninghua Tan
Keyword(s):  
Hairy Root ◽  
Root Culture ◽  
Hairy Root Culture

Hairy Root Culture for In Vitro Production of Secondary Metabolites: A Promising Biotechnological Approach

2018 ◽  
pp. 235-250
Author(s):  
Ravi Shankar Singh ◽  
Tirthartha Chattopadhyay ◽  
Dharamsheela Thakur ◽  
Nitish Kumar ◽  
Tribhuwan Kumar ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Hairy Root ◽  
Root Culture ◽  
Hairy Root Culture ◽  
In Vitro Production ◽  
Biotechnological Approach ◽  
Vitro Production

Oxygen Effects in Hairy Root Culture

Hairy Roots ◽  
2020 ◽  
pp. 140-150
Author(s):  
Shaoxiong Yu ◽  
M.G.P. Mahagamasekera ◽  
Gary R.C. Williams ◽  
Kanokwan Kanokwaree ◽  
Pauline M. Doran
Keyword(s):  
Hairy Root ◽  
Root Culture ◽  
Hairy Root Culture ◽  
Oxygen Effects

scholarly journals Establishment of the hairy root culture of Echium plantagineum L. and its shikonin production

3 Biotech ◽  
2020 ◽  
Vol 10 (10) ◽  
Author(s):  
Jiang-Yan Fu ◽  
Hua Zhao ◽  
Jia-Xin Bao ◽  
Zhong-Ling Wen ◽  
Rong-Jun Fang ◽  
...  
Keyword(s):  
Hairy Root ◽  
Root Culture ◽  
Hairy Root Culture

scholarly journals Prospects of Plumbago rosea L. hairy root culture in traditional preparations: a phytochemical comparison with tuberous roots

Plant Root ◽  
2014 ◽  
Vol 8 (0) ◽  
pp. 13-23 ◽  
Author(s):  
K. Satheeshkumar ◽  
Binoy Jose ◽  
Dhanya Pillai ◽  
P. N. Krishnan
Keyword(s):  
Hairy Root ◽  
Root Culture ◽  
Hairy Root Culture ◽  
Tuberous Roots ◽  
Plumbago Rosea
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