Advances in Cell Seeding of Tissue Engineered Vascular Grafts

2020 ◽  
pp. 1-25
Author(s):  
Justin S. Weinbaum ◽  
Darren G. Haskett ◽  
Talya F. Mandelkern ◽  
David A. Vorp
Keyword(s):  
Vascular Grafts ◽  
Cell Seeding

Endothelial cell seeding of vascular grafts: Experimental investigations and clinical update

1996 ◽  
Vol 5 (5) ◽  
pp. 296 ◽  
Author(s):  
Manfred Deutsch ◽  
Johann Meinhart ◽  
Teddy Fischlein ◽  
Peter Zilla
Keyword(s):  
Endothelial Cell ◽  
Vascular Grafts ◽  
Experimental Investigations ◽  
Cell Seeding ◽  
Endothelial Cell Seeding

Endothelial Cell Seeding on PTFE Vascular Prostheses Using a Standardized Seeding Technique

1989 ◽  
Vol 12 (4) ◽  
pp. 270-275 ◽  
Author(s):  
J. Gerlach ◽  
K.-M. Kreusel ◽  
H.H. Schauwecker ◽  
E.S. Bücherl
Keyword(s):  
Endothelial Cells ◽  
Rotation Speed ◽  
Vascular Grafts ◽  
Cell Number ◽  
Cell Seeding ◽  
Vascular Prostheses ◽  
Adhesion Rate ◽  
Endothelial Cell Seeding ◽  
Kinetics Of ◽  
Graft Diameter

A standardized method was developed for seeding endothelial cells (EC) in tubular vascular grafts. A rotational cell seeding device for tubular prostheses is presented and parameters influencing the kinetics of cell adhesion (rotation speed, graft diameter, cell suspension level, inoculated cell number) are reported. Seeding EC in 14 mm ID PTFE vascular grafts with rotation rate of 10 rph gave an adhesion rate of 80% in a homogeneous monolayer.

Microvascular Endothelial Cell Seeding of Small-Diameter Dacron|2TM|0 Vascular Grafts

1988 ◽  
Vol 1 (1) ◽  
pp. 35-44 ◽  
Author(s):  
Steven P. Schmidt ◽  
Navid Monajjem ◽  
M. Michelle Evancho ◽  
Todd R. Pippert ◽  
W. V. Sharp
Keyword(s):  
Endothelial Cell ◽  
Vascular Grafts ◽  
Small Diameter ◽  
Microvascular Endothelial Cell ◽  
Cell Seeding ◽  
Endothelial Cell Seeding ◽  
Microvascular Endothelial

Fibrinolytic profiles after endothelial cell seeding of prosthetic vascular grafts

1994 ◽  
Vol 8 ◽  
pp. 139
Author(s):  
J.V. Smyth ◽  
M. Welch ◽  
H.M.H. Carr ◽  
P. Eisenberg ◽  
M.G. Walker
Keyword(s):  
Endothelial Cell ◽  
Vascular Grafts ◽  
Cell Seeding ◽  
Endothelial Cell Seeding

Endothelial Cell Seeding on Crosslinked Collagen: Effects of Crosslinking on Endothelial Cell Proliferation and Functional Parameters

2000 ◽  
Vol 84 (08) ◽  
pp. 325-331 ◽  
Author(s):  
M. J. B. Wissink ◽  
M. J. A. van Luyn ◽  
R. Beernink ◽  
F. Dijk ◽  
A. A. Poot ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Endothelial Cell ◽  
Plasminogen Activator ◽  
Type I Collagen ◽  
Vascular Grafts ◽  
Small Diameter ◽  
Endothelial Cell Proliferation ◽  
Type I ◽  
Cell Seeding ◽  
Endothelial Cell Seeding

SummaryEndothelial cell seeding, a promising method to improve the performance of small-diameter vascular grafts, requires a suitable substrate, such as crosslinked collagen. Commonly used crosslinking agents such as glutaraldehyde and formaldehyde cause, however, cytotoxic reactions and thereby hamper endothelialization of currently available collagen-coated vascular graft materials.The aim of this study was to investigate the effects of an alternative method for crosslinking of collagen, using N-(3-dimethylaminopropyl)-N’-ethylcarbodiimide (EDC) in combination with N-hydroxysuccinimide (NHS), on various cellular functions of human umbilical vein endothelial cells (HUVECs) in vitro. Compared to non-crosslinked type I collagen, proliferation of seeded endothelial cells was significantly increased on EDC/NHS-crosslinked collagen. Furthermore, higher cell numbers were found with increasing crosslink densities. Neither the morphology of the cells nor the secretion of prostacyclin (PGI2), von Willebrand factor (vWF), tissue plasminogen activator (t-PA) and plasminogen activator inhibitor (PAI-1) was affected by the crosslink density of the collagen substrate. Therefore, EDC/NHScrosslinked collagen is candidate substrate for in vivo application such as endothelial cell seeding of collagen-coated vascular grafts.

Dynamic Straining Combined with Fibrin Gel Cell Seeding Improves Strength of Tissue-Engineered Small-Diameter Vascular Grafts

2009 ◽  
Vol 15 (5) ◽  
pp. 1081-1089 ◽  
Author(s):  
Maria Stekelenburg ◽  
Marcel C.M. Rutten ◽  
Luc H.E.H. Snoeckx ◽  
Frank P.T. Baaijens
Keyword(s):  
Vascular Grafts ◽  
Small Diameter ◽  
Cell Seeding ◽  
Fibrin Gel ◽  
Small Diameter Vascular Grafts

114. Evaluation of the Role of Selective Cell Seeding on Neovessel Formation in Tissue Engineered Vascular Grafts

2009 ◽  
Vol 151 (2) ◽  
pp. 222-223
Author(s):  
T.L. Mirensky ◽  
N. Hibino ◽  
R. Sawh-Martinez ◽  
M.P. Brennan ◽  
T. Yi ◽  
...  
Keyword(s):  
Vascular Grafts ◽  
Cell Seeding

Endothelial cell seeding of polytetrafluoroethylene vascular grafts in humans: A preliminary report

1987 ◽  
Vol 6 (6) ◽  
pp. 535-541 ◽  
Author(s):  
Peter Zilla ◽  
Roland Fasol ◽  
Manfred Deutsch ◽  
Teddy Fischlein ◽  
Erich Minar ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Preliminary Report ◽  
Vascular Grafts ◽  
Cell Seeding ◽  
Endothelial Cell Seeding

scholarly journals Tissue-engineered vascular grafts: does cell seeding matter?

2010 ◽  
Vol 45 (6) ◽  
pp. 1299-1305 ◽  
Author(s):  
Tamar L. Mirensky ◽  
Narutoshi Hibino ◽  
Rajendra F. Sawh-Martinez ◽  
Tai Yi ◽  
Gustavo Villalona ◽  
...  
Keyword(s):  
Vascular Grafts ◽  
Cell Seeding
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