Anisotropic Conductivity Imaging with MREIT Using Equipotential Projection Algorithm

2007 ◽  
pp. 496-499 ◽  
Author(s):  
B.M. Eyüboğlu ◽  
E. Değirmenci
Keyword(s):  
Projection Algorithm ◽  
Anisotropic Conductivity ◽  
Conductivity Imaging

Electron Microscopy and image reconstruction reveal the structural basis for spectrin's elastic properties

1992 ◽  
Vol 50 (1) ◽  
pp. 510-511
Author(s):  
Amy M. McGough ◽  
Robert Josephs
Keyword(s):  
Electron Microscopy ◽  
Elastic Properties ◽  
Conformational Changes ◽  
Quaternary Structure ◽  
Three Dimensional ◽  
Membrane Skeleton ◽  
Projection Algorithm ◽  
Structural Basis ◽  
Iterative Approximation ◽  
Membrane Skeletons

The remarkable deformability of the erythrocyte derives in large part from the elastic properties of spectrin, the major component of the membrane skeleton. It is generally accepted that spectrin's elasticity arises from marked conformational changes which include variations in its overall length (1). In this work the structure of spectrin in partially expanded membrane skeletons was studied by electron microscopy to determine the molecular basis for spectrin's elastic properties. Spectrin molecules were analysed with respect to three features: length, conformation, and quaternary structure. The results of these studies lead to a model of how spectrin mediates the elastic deformation of the erythrocyte.Membrane skeletons were isolated from erythrocyte membrane ghosts, negatively stained, and examined by transmission electron microscopy (2). Particle lengths and end-to-end distances were measured from enlarged prints using the computer program MACMEASURE. Spectrin conformation (straightness) was assessed by calculating the particles’ correlation length by iterative approximation (3). Digitised spectrin images were correlation averaged or Fourier filtered to improve their signal-to-noise ratios. Three-dimensional reconstructions were performed using a suite of programs which were based on the filtered back-projection algorithm and executed on a cluster of Microvax 3200 workstations (4).

Anisotropic conductivity for single-cell electroporation simulation with tangentially dispersive membrane

2021 ◽  
pp. 138426
Author(s):  
Fei Guo ◽  
Kun Qian ◽  
Lin Zhang ◽  
Hao Deng ◽  
Xin Li ◽  
...  
Keyword(s):  
Single Cell ◽  
Anisotropic Conductivity ◽  
Cell Electroporation ◽  
Single Cell Electroporation
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