Simple Evolution of Complex Crystal Species

This tutorial will discuss the methodology of low dose electron diffraction and imaging of crystalline biological objects, the problems of data interpretation for two-dimensional projected density maps of glucose embedded protein crystals, the factors to be considered in combining tilt data from three-dimensional crystals, and finally, the prospects of achieving a high resolution three-dimensional density map of a biological crystal. This methodology will be illustrated using two proteins under investigation in our laboratory, the T4 DNA helix destabilizing protein gp32*I and the crotoxin complex crystal.

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Eels Under Standing Wave Conditions

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100054911 ◽

1982 ◽

Vol 40 ◽

pp. 492-495

Author(s):

O.L. Krivanek ◽

J. TaftØ

Keyword(s):

Standing Wave ◽

Wave Conditions ◽

Set Up ◽

A Site ◽

Complex Crystal

It is well known that a standing electron wavefield can be set up in a crystal such that its intensity peaks at the atomic sites or between the sites or in the case of more complex crystal, at one or another type of a site. The effect is usually referred to as channelling but this term is not entirely appropriate; by analogy with the more established particle channelling, electrons would have to be described as channelling either through the channels or through the channel walls, depending on the diffraction conditions.

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Spot-scan imaging of ice-embedded catalase crystal on a slow-scan CCD camera in a 400-kV electron cryomicroscope

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100168220 ◽

1994 ◽

Vol 52 ◽

pp. 98-99

Author(s):

Wah Chiu ◽

Michael Sherman ◽

Jaap Brink

Keyword(s):

Electron Diffraction ◽

Ccd Camera ◽

Protein Crystal ◽

Modulation Transfer ◽

Measured Intensity ◽

3 Dimensional ◽

Dimensional Reconstruction ◽

Diffraction Patterns ◽

Complex Crystal

In protein electron crystallography, both low dose electron diffraction patterns and images are needed to provide accurate amplitudes and phases respectively for a 3-dimensional reconstruction. We have demonstrated that the Gatan 1024x1024 model 679 slow-scan CCD camera is useful to record electron diffraction intensities of glucose-embedded crotoxin complex crystal to 3 Å resolution. The quality of the electron diffraction intensities is high on the basis of the measured intensity equivalence ofthe Friedel-related reflections. Moreover, the number of patterns recorded from a single crystal can be as high as 120 under the constraints of radiation damage and electron statistics for the reflections in each pattern.A limitation of the slow-scan CCD camera for recording electron images of protein crystal arises from the relatively large pixel size, i.e. 24 μm (provided by Gatan). The modulation transfer function of our camera with a P43 scintillator has been determined for 400 keV electrons and shows an amplitude fall-off to 0.25 at 1/60 μm−1.

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Faculty Opinions recommendation of The yeast ski complex: crystal structure and RNA channeling to the exosome complex.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.718077709.793483675 ◽

2013 ◽

Author(s):

Ben Luisi ◽

Katarzyna Bandyra

Keyword(s):

Crystal Structure ◽

Complex Crystal Structure ◽

Complex Crystal ◽

Exosome Complex

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Thiophene-containing thiolato dimers, oxygen inserted Cu(II) complex, crystal structures, molecular docking and theoretical studies

Journal of Coordination Chemistry ◽

10.1080/00958972.2016.1192611 ◽

2016 ◽

Vol 69 (11-13) ◽

pp. 2015-2023 ◽

Cited By ~ 2

Author(s):

Shaikh M. Mobin ◽

Mohd. Tauqeer ◽

Akbar Mohammad ◽

Veenu Mishra ◽

Pratibha Kumari

Keyword(s):

Molecular Docking ◽

Crystal Structures ◽

Theoretical Studies ◽

Complex Crystal

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Vapochromism induced by intermolecular electron transfer coupled with hydrogen-bond formation in zinc dithiolene complex

Journal of Materials Chemistry C ◽

10.1039/d0tc04280c ◽

2020 ◽

Vol 8 (42) ◽

pp. 14939-14947

Author(s):

So Yokomori ◽

Shun Dekura ◽

Tomoko Fujino ◽

Mitsuaki Kawamura ◽

Taisuke Ozaki ◽

...

Keyword(s):

Hydrogen Bond ◽

Electron Transfer ◽

Bond Formation ◽

Intermolecular Electron Transfer ◽

Hydrogen Bond Formation ◽

Complex Crystal

A novel vapochromic mechanism by intermolecular electron transfer coupled with hydrogen-bond formation was realized in a zinc dithiolene complex crystal.

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