Expression Analysis of Mitochondrial Components in a Variety of Plant Species Using Real-Time Quantitative PCR

2004 ◽  
pp. 61-72
Author(s):  
Katharine A. Howell ◽  
Ryan Lister ◽  
James Whelan
Keyword(s):  
Real Time ◽  
Quantitative Pcr ◽  
Plant Species ◽  
Expression Analysis ◽  
Real Time Quantitative Pcr

scholarly journals Identification of reference genes for expression analysis by real-time quantitative PCR in drought-stressed soybean

2011 ◽  
Vol 46 (1) ◽  
pp. 58-65 ◽  
Author(s):  
Renata Stolf-Moreira ◽  
Eliana Gertrudes de Macedo Lemos ◽  
Ricardo Vilela Abdelnoor ◽  
Magda Aparecida Beneventi ◽  
Amanda Alves Paiva Rolla ◽  
...  
Keyword(s):  
Real Time ◽  
Quantitative Pcr ◽  
Reference Gene ◽  
Expression Analysis ◽  
Stable Gene ◽  
Expression Stability ◽  
Hydroponic System ◽  
Mean Values ◽  
Real Time Quantitative Pcr ◽  
Ct Data

The objective of this work was to validate, by quantitative PCR in real time (RT-qPCR), genes to be used as reference in studies of gene expression in soybean in drought-stressed trials. Four genes commonly used in soybean were evaluated: Gmβ-actin, GmGAPDH, GmLectin and GmRNAr18S. Total RNA was extracted from six samples: three from roots in a hydroponic system with different drought intensities (0, 25, 50, 75 and 100 minutes of water stress), and three from leaves of plants grown in sand with different soil moistures (15, 5 and 2.5% gravimetric humidity). The raw cycle threshold (Ct) data were analyzed, and the efficiency of each primer was calculated for an overall analysis of the Ct range among the different samples. The GeNorm application was used to evaluate the best reference gene, according to its stability. The GmGAPDH was the least stable gene, with the highest mean values of expression stability (M), and the most stable genes, with the lowest M values, were the Gmβ-actin and GmRNAr18S, when both root and leaves samples were tested. These genes can be used in RT-qPCR as reference gene for expression analysis.

scholarly journals Quantification and Stability of Human Adenoviruses and Polyomavirus JCPyV in Wastewater Matrices

2006 ◽  
Vol 72 (12) ◽  
pp. 7894-7896 ◽  
Author(s):  
Silvia Bofill-Mas ◽  
Nestor Albinana-Gimenez ◽  
Pilar Clemente-Casares ◽  
Ayalkibet Hundesa ◽  
Jesus Rodriguez-Manzano ◽  
...  
Keyword(s):  
Real Time ◽  
Quantitative Pcr ◽  
High Stability ◽  
Wastewater Sludge ◽  
Human Polyomavirus ◽  
Sewage Effluent ◽  
Viral Contamination ◽  
Human Adenoviruses ◽  
Urban Sewage ◽  
Real Time Quantitative Pcr

ABSTRACT Human adenoviruses (HAdV) and human polyomavirus JCPyV have been previously proposed as indicators of fecal viral contamination in the environment. Different wastewater matrices have been analyzed by applying real-time quantitative PCR procedures for the presence, quantity, and stability of a wide diversity of excreted HAdV and JCPyV. High quantities of HAdV and JCPyV were detected in sewage, effluent wastewater, sludge, and biosolid samples. Both viruses showed high stability in urban sewage. These results confirm the suitability of both viruses as indicators of human fecal viral pollution.

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