English reading teaching in college is an important aspect in cultivating students' listening, speaking, reading and writing skills, as well as a vital way to cultivate students' ability of using language. Many scholars have proved that language teaching and culture teaching are inseparable in reading teaching. The cultural penetration in English is emphasized by language researchers. However, a number of teachers pay more attention to words and difficult sentences in English reading teaching, they ignore Chinese traditional culture factors in reading materials and the importance of the traditional Chinese culture teaching in college. college stage is a key period of language learning. At this period, they should gradually understand Chinese traditional culture. It is a prerequisite for college students to strengthen their Chinese cultural knowledge in English learning. However, there are a few studies on the penetration of traditional Chinese culture in college English reading teaching. According to this situation, the author consults many literature surveys of recent years. From the viewpoints of students, teachers and teaching materials, the paper shows the present situation of the Chinese cultural penetration in college English reading teaching. According to the results of study, the paper puts forward some relevant teaching principles and approaches about how to conduct Chinese cultural penetration in English reading teaching.