Effect of Intermittent Fluid Shear Stress on Cardiomyogenic Differentiation of Rat Bone Marrow Mesenchymal Stem Cells

2013 ◽  
pp. 1953-1956 ◽  
Author(s):  
Yan Huang ◽  
Yubo Fan
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Shear Stress ◽  
Mesenchymal Stem Cells ◽  
Fluid Shear Stress ◽  
Fluid Shear ◽  
Cardiomyogenic Differentiation ◽  
Marrow Mesenchymal Stem Cells ◽  
Rat Bone

scholarly journals Stable changing fluid shear stress promotes osteogenesis

2018 ◽  
Author(s):  
Linyuan Wang ◽  
Fang Xiao ◽  
Lei Yu ◽  
Hang Su ◽  
Yueqin Yang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Shear Stress ◽  
Mesenchymal Stem Cells ◽  
Fluid Shear Stress ◽  
Nuclear Factor ◽  
Mechanical Stimuli ◽  
Fluid Shear ◽  
Receptor Activator ◽  
Nuclear Factor Κ B

AbstractObjectiveBone marrow cells encounter various chemical and mechanical stimuli from the internal environment. In vivo, fluid shear stress (FSS) is one of the primary mechanical stimuli that affect bone marrow-derived mesenchymal stem cells (BMCs) activity. Since various cases of FSS influence BMCs activity differently, the purpose of this study is to determine how BMC activity in osteogenesis and osteoclastgenesis is affected by stable and unstable changing FSS.MethodBMCs samples from the femur of the mouse, divided in to three groups: stimulate by stable changing FSS, unstable changing FSS, and no FSS. RT-PCR would be applied to detect OPG, RANKL, ALP, OCN, RUNX2, and RANK of all the samples at the 3rd day. Alizarin red staining and TRAP staining would be applied to test all the samples at the 6th day.ResultsThe S group samples showed the lowest level in RANKL mRNA and showed the highest level in OPG mRNA. RANKL/OPG mRNA in the S group was the smallest during three groups. Comparing with the C group samples, RUNX2 mRNA in the S group was increased significantly. RANK mRNA in the C group was three times more than S group. S group had the largest area of mineralized nodules, and largest number of area ≥100µm, ≥500µm2 or ≥1000µm2. The area of positive TRAP stain in S group was the smallest among three groups.ConclusionStable changing FSS significantly increases osteogenesis relating to OPG-RANKL-RANK pathway. Compared with unstable changing FSS, stable changing FSS afford a more appropriate stimulation to osteogenesis.AbbreviationsALPAlkaline phosphataseBMCsBone marrow-derived mesenchymal stem cellsCx43Connexin 43C3aComplement Component 3aJNKc-Jun N-terminal kinaseERKExtracellular regulated protein kinasesERK5Extracellular-signal-regulated kinaseFSSFluid shear stressFasLFirst apoptosis signal LigandGSK3βGlycogen synthase kinase 3βLPALysophosphatidic acidMSCsMesenchymal stem cellsM-CSFMacrophage colony-stimulating factorMCP-1Monocyte chemoattractant protein-1RANKReceptor Activator of Nuclear Factor κ BRANKLReceptor activator of nuclear factor κ B ligandRUNX2Runt-related transcription factor 2P2Y2RReceptor of P2Y2LGR4Repeat-containing G-protein-coupled receptor 4SemaSemaphorinsTRAPTartrate-Resistant Acid AhosphataseTRPM7Transient receptor potential cation channel subfamily M member 7TRAFsTumornecrosisfactorreceptor associatedfactorsOPGOsteoprotegerinOCNOsteocalcinOPNOsteopontin

scholarly journals Hyaluronan Synthases’ Expression and Activity Are Induced by Fluid Shear Stress in Bone Marrow-Derived Mesenchymal Stem Cells

2021 ◽  
Vol 22 (6) ◽  
pp. 3123
Author(s):  
Sebastian Reiprich ◽  
Elif Akova ◽  
Attila Aszódi ◽  
Veronika Schönitzer
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Shear Stress ◽  
Mesenchymal Stem Cells ◽  
Fluid Shear Stress ◽  
Regulatory Function ◽  
Fluid Shear ◽  
Chitin Synthases ◽  
Hyaluronan Synthases ◽  
Expression And Activity

During biomineralization, the cells generating the biominerals must be able to sense the external physical stimuli exerted by the growing mineralized tissue and change their intracellular protein composition according to these stimuli. In molluscan shell, the myosin-chitin synthases have been suggested to be the link for this communication between cells and the biomaterial. Hyaluronan synthases (HAS) belong to the same enzyme family as chitin synthases. Their product hyaluronan (HA) occurs in the bone and is supposed to have a regulatory function during bone regeneration. We hypothesize that HASes’ expression and activity are controlled by fluid-induced mechanotransduction as it is known for molluscan chitin synthases. In this study, bone marrow-derived human mesenchymal stem cells (hMSCs) were exposed to fluid shear stress of 10 Pa. The RNA transcriptome was analyzed by RNA sequencing (RNAseq). HA concentrations in the supernatants were measured by ELISA. The cellular structure of hMSCs and HAS2-overexpressing hMSCs was investigated after treatment with shear stress using confocal microscopy. Fluid shear stress upregulated the expression of genes that encode proteins belonging to the HA biosynthesis and bone mineralization pathways. The HAS activity appeared to be induced. Knowledge about the regulation mechanism governing HAS expression, trafficking, enzymatic activation and quality of the HA product in hMSCs is essential to understand the biological role of HA in the bone microenvironment.

Dosage and Passage Dependent Neuroprotective Effects of Exosomes Derived from Rat Bone Marrow Mesenchymal Stem Cells: An In Vitro Analysis

2018 ◽  
Vol 18 ◽  
Author(s):  
Chaitra Venugopal ◽  
Christopher Shamir ◽  
Sivapriya Senthilkumar ◽  
Janitri Venkatachala Babu ◽  
Peedikayil Kurien Sonu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Neuroprotective Effects ◽  
Marrow Mesenchymal Stem Cells ◽  
Vitro Analysis ◽  
In Vitro Analysis ◽  
Rat Bone

The rate of fluid shear stress is a potent regulator for the differentiation of mesenchymal stem cells

2019 ◽  
Vol 234 (9) ◽  
pp. 16312-16319 ◽  
Author(s):  
Danyang Yue ◽  
Mengxue Zhang ◽  
Juan Lu ◽  
Jin Zhou ◽  
Yuying Bai ◽  
...  
Keyword(s):  
Stem Cells ◽  
Shear Stress ◽  
Mesenchymal Stem Cells ◽  
Fluid Shear Stress ◽  
Fluid Shear
Sign in / Sign up

Export Citation Format

Share Document