Tissue Engineering of Vascularized Adipose Tissue for Soft Tissue Reconstruction

2014 ◽  
pp. 23-40
Author(s):  
Silvan M. Klein ◽  
Jody Vykoukal ◽  
Lukas Prantl ◽  
Juergen H. Dolderer
Keyword(s):  
Tissue Engineering ◽  
Adipose Tissue ◽  
Soft Tissue ◽  
Soft Tissue Reconstruction ◽  
Tissue Reconstruction

scholarly journals An immunologically active, adipose-derived extracellular matrix biomaterial for soft tissue reconstruction: concept to clinical trial

2022 ◽  
Vol 7 (1) ◽  
Author(s):  
Amy E. Anderson ◽  
Iwen Wu ◽  
Alexis J. Parrillo ◽  
Matthew T. Wolf ◽  
David R. Maestas ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Adipose Tissue ◽  
Soft Tissue ◽  
Human Subjects ◽  
Donor Site ◽  
Tissue Remodeling ◽  
Interleukin 4 ◽  
Soft Tissue Reconstruction ◽  
Fat Grafts ◽  
Tissue Reconstruction

AbstractSoft tissue reconstruction remains an intractable clinical challenge as current surgical options and synthetic implants may produce inadequate outcomes. Soft tissue deficits may be surgically reconstructed using autologous adipose tissue, but these procedures can lead to donor site morbidity, require multiple procedures, and have highly variable outcomes. To address this clinical need, we developed an “off-the-shelf” adipose extracellular matrix (ECM) biomaterial from allograft human tissue (Acellular Adipose Tissue, AAT). We applied physical and chemical processing methods to remove lipids and create an injectable matrix that mimicked the properties of lipoaspirate. Biological activity was assessed using cell migration and adipogenesis assays. Characterization of regenerative immune properties in a murine muscle injury model revealed that allograft and xenograft AAT induced pro-regenerative CD4+ T cells and macrophages with xenograft AAT additionally attracting eosinophils secreting interleukin 4 (Il4). In immunocompromised mice, AAT injections retained similar volumes as human fat grafts but lacked cysts and calcifications seen in the fat grafts. The combination of AAT with human adipose-derived stem cells (ASCs) resulted in lower implant volumes. However, tissue remodeling and adipogenesis increased significantly in combination with ASCs. Larger injected volumes of porcine-derived AAT demonstrated biocompatibility and greater retention when applied allogeneicly in Yorkshire cross pigs. AAT was implanted in healthy volunteers in abdominal tissue that was later removed by elective procedures. AAT implants were well tolerated in all human subjects. Implants removed between 1 and 18 weeks demonstrated increasing cellular infiltration and immune populations, suggesting continued tissue remodeling and the potential for long-term tissue replacement.

Acellular Adipose Tissue Scaffold for Soft Tissue Reconstruction

2011 ◽  
Vol 128 ◽  
pp. 83-84 ◽  
Author(s):  
Iwen Wu ◽  
Zayna Nahas ◽  
Gedge Rosson ◽  
Jennifer Elisseeff
Keyword(s):  
Adipose Tissue ◽  
Soft Tissue ◽  
Soft Tissue Reconstruction ◽  
Tissue Scaffold ◽  
Tissue Reconstruction

“Smart” Autologous Tissue Engineering for Soft Tissue Reconstruction

2006 ◽  
Vol 22 (03) ◽  
Author(s):  
J.J. Vranckx ◽  
P. Vermeulen ◽  
S. Dickens ◽  
H. Devroe ◽  
P. Massage ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Soft Tissue ◽  
Soft Tissue Reconstruction ◽  
Autologous Tissue ◽  
Tissue Reconstruction

scholarly journals An immunologically active, adipose-derived extracellular matrix biomaterial for soft tissue reconstruction: concept to clinical trial

2020 ◽  
Author(s):  
Amy E. Anderson ◽  
Iwen Wu ◽  
Alexis J. Parrillo ◽  
David R. Maestas ◽  
Ian Graham ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Soft Tissue ◽  
Human Subjects ◽  
Donor Site ◽  
Tissue Remodeling ◽  
Fat Grafting ◽  
Interleukin 4 ◽  
Fat Graft ◽  
Soft Tissue Reconstruction ◽  
Tissue Reconstruction

ABSTRACTSoft tissue reconstruction remains an intractable clinical challenge as current surgical options and synthetic implants may produce inadequate outcomes. Soft tissue deficits may be surgically reconstructed using autologous adipose tissue, but these procedures can lead to donor site morbidity, require multiple trips to the operating room, and have highly variable outcomes. To address the clinical need for soft tissue reconstruction, we developed an “off-the-shelf” adipose matrix from allograft human adipose tissue (acellular adipose tissue, AAT). We applied physical and chemical processing methods to remove lipids and create an injectable matrix that mimicked the properties of fat grafting materials. Biological activity was assessed using cell migration and stem cell adipogenesis assays. Characterization of the regenerative immunology properties in a murine muscle injury model revealed allograft and xenograft AAT induced pro-regenerative CD4+ T cells and macrophages with xenograft AAT attracting additional eosinophils secreting interleukin 4 (Il4). In immunocompromised mice, AAT injections retained similar tissue volumes as human fat grafts but did not have the cysts and calcifications that formed in the human fat graft implants. Combination of AAT with human adipose-derived stem cells (ASCs) resulted in lower implant volumes. However, tissue remodeling and new adipose development increased significantly with the addition of cells. Larger injected volumes of porcine-derived AAT demonstrated biocompatibility and greater volume retention when applied allogeneicly in Yorkshire cross pigs. Under a biologic IND application, AAT was implanted in healthy volunteers in abdominal tissue that was later removed (panniculectomy or abdominoplasty). The AAT implants were well tolerated and biocompatible in all eight human subjects. Analysis of implants removed between 1 and 18 weeks demonstrated increasing cellular infiltration and immune populations, suggesting continued tissue remodeling and the potential for long term tissue replacement.SUMMARYAn adipose-derived injectable biomaterial provides volume correction for soft tissue defects while promoting pro-healing immune responses.

Autologous Fat Transfer and Cell-Based Strategies for Soft Tissue Reconstruction

2014 ◽  
Vol 3 (1) ◽  
pp. 62-69
Author(s):  
Kshemendra Senarath-Yapa ◽  
Rebecca Garza ◽  
Adrian McArdle ◽  
Graham Walmsley ◽  
Michael Hu ◽  
...  
Keyword(s):  
Soft Tissue ◽  
Soft Tissue Reconstruction ◽  
Fat Transfer ◽  
Autologous Fat ◽  
Autologous Fat Transfer ◽  
Tissue Reconstruction

scholarly journals Radical treatment of severe open fractures of extremities by orthoplastic surgery: a 10-year retrospective study

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Zhao Yang ◽  
Chao Xu ◽  
Yong-Gang Zhu ◽  
Jun Li ◽  
Zi-Xiang Wu ◽  
...  
Keyword(s):  
Soft Tissue ◽  
Infection Rate ◽  
Clinical Data ◽  
Open Fractures ◽  
Soft Tissue Reconstruction ◽  
Satisfactory Outcome ◽  
Tissue Damages ◽  
Tissue Reconstruction ◽  
Severe Soft Tissue

Abstract Objective This study aimed to retrospectively analyze clinical data of a series of patients with severe open fractures of extremities (Gustilo IIIb or IIIc), who achieved a satisfactory outcome through radical orthoplastic surgery, so as to provide a reference for determining the treatment of severe open fractures of extremities. Methods The clinical data of 41 consecutive patients with severe open fracture (Gustilo IIIb or IIIc) of the limb, who underwent successful surgical debridement, fixation, and soft tissue reconstruction in one stage between January 2008 and January 2019, were retrospectively reviewed. Postoperative indicators, including infection rate and union time, were acquired by a regular follow-up and analyzed. Results The mean (±SD) age of the patients was 38 ± 16 years. A total of 90 open fractures and severe soft tissue damages were analyzed. The soft tissue cover was achieved within 72 h. The overall rate of infection was 14.6% (6/41). Sex and the Mangled Extremity Severity Score were associated with infection. The median union time of 40 patients (one amputation) was 32 weeks. Conclusion The overall rate of infection exhibited a lower tendency in this study compared with previous studies on high-grade open fractures following a two-stage orthopedic approach. The consequence of infection rate and union time was similar to that in previous studies. These results indicated that the single-stage radical orthoplastic treatment was an effective and reliable option for reconstructing severe open fractures.

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