The physiological activity of twenty one 9-amine-9-fluorenephosphonic acid derivatives was tested. The compounds were synthesized by the Wrocław Technical University. <i>Spirodela oligorrhiza</i> was used as the test plant. There was tested the inhibiting influence of these compounds on the growth of <i>Spirodela</i> and synergism with the herbicide "mecoprop". Ethyl esters substituted at the nitrogen atom with buthyl and heptyl group appeared to be most active. Their concentration of 0.5-1.5 × 10<sup>-5</sup> M inhibited the growth of <i>Spirodela</i> by 50%. Most of the 9-amine-9-fluorenephosphonic acid derivatives tested with different substitutes showed a strong synergism with the herbicide "mecoprop". An addition of these compounds to sublethal solution of the herbicide killed <i>Spirodela</i> within 10-20 houres.