Rapid Detection of Hepatitis C Virus in Plasma and Liver Biopsies by Capillary Electrophoresis

1998 ◽  
pp. 193-201
Author(s):  
Sameer A. Sakallah ◽  
Robert W. Lanning ◽  
David L. Cooper
Keyword(s):  
Capillary Electrophoresis ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Rapid Detection ◽  
Liver Biopsies

scholarly journals Detection and Localization by In Situ Molecular Biology Techniques and Immunohistochemistry of Hepatitis C Virus in Livers of Chronically Infected Patients

1998 ◽  
Vol 46 (5) ◽  
pp. 653-660 ◽  
Author(s):  
Francine M. Walker ◽  
Marie-Christine Dazza ◽  
Marie-Christine Dauge ◽  
Olivier Boucher ◽  
Christophe Bedel ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Interferon Α ◽  
Rt Pcr ◽  
Biopsy Specimens ◽  
Bile Ductule ◽  
Liver Biopsies ◽  
Cytoplasmic Signals ◽  
Detection And Localization

Hepatitis C virus (HCV) detection in the livers of chronically infected patients remains a debatable issue. We used immunohistochemistry, in situ hybridization (ISH) alone or after microwave heating with FITC-labeled probes, RT-PCR with unlabeled primers followed by ISH (RT-PCR-ISH), and in situ RT-PCR with FITC-labeled primers (in situ RT-PCRd) to localize the virus in 38 liver biopsy specimens from 21 chronically infected HCV patients treated with interferon-α (IFN-α). Biopsies were taken at the beginning and end of IFN-α treatment and 1 year later. Results were compared with that of HCV-PCR in serum. RT-PCR-ISH and in situ RT-PCRd showed HCV signal in all liver biopsies even in responders with seronegative HCV PCR. This signal was intranuclear, diffuse, or peripheral, in hepatocytes, bile ductule cells, and lymphocytes. Cytoplasmic signals were occasionally observed. Whereas the percentage of labeled hepatocytes remained constant, the number of labeled lymphoid follicles decreased after INF-α therapy. Immunohistochemistry resulted in the same pattern of positivity but it was weaker and inconstant. This study indicates the persistency of HCV latency in IFN-α responders 1 year after IFN-α treatment cessation, a finding that certainly deserves confirmation.

scholarly journals Fully automated rapid quantification of Hepatitis C Virus RNA in human plasma and serum by integrated on-chip RT-qPCR and capillary electrophoresis

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Samuel D. H. Chan ◽  
Hidenori Toyoda ◽  
Jayashree Sanjeeviraman ◽  
Aurelie Souppe ◽  
Mari Iwamoto ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Human Plasma ◽  
Hepatitis C Virus Rna ◽  
Virus Rna ◽  
On Chip ◽  
Rapid Quantification

Clinical value and safety of liver biopsies in patients transplanted for hepatitis C virus-related end-stage liver disease

2014 ◽  
Vol 16 (6) ◽  
pp. 958-967
Author(s):  
C. Brauner ◽  
T.O. Lankisch ◽  
P. Fytili ◽  
J. Jaroszewicz ◽  
F. Lehner ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Liver Disease ◽  
Hepatitis C ◽  
Clinical Value ◽  
End Stage Liver Disease ◽  
Liver Biopsies ◽  
End Stage

Effects of delayed freezing of liver biopsies on the detection of hepatitis C virus RNA strands

2000 ◽  
Vol 32 (6) ◽  
pp. 1019-1025 ◽  
Author(s):  
Antonio Madejón ◽  
Maria Luisa Manzano ◽  
Carlos Arocena ◽  
Inmaculada Castillo ◽  
Vicente Carreño
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Hepatitis C Virus Rna ◽  
Virus Rna ◽  
Liver Biopsies

Evaluation of Methods for Hepatitis C Virus Detection in Archival Liver Biopsies

1994 ◽  
Vol 190 (11) ◽  
pp. 1017-1025 ◽  
Author(s):  
P. Komminoth ◽  
V. Adams ◽  
A.A. Long ◽  
J. Roth ◽  
P. Saremaslani ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Virus Detection ◽  
Liver Biopsies ◽  
Evaluation Of Methods
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