In vivo Measurements of Ionized Mg2+ and Ca2+ in Single Cells

1977 ◽  
pp. 552-566 ◽  
Author(s):  
A. Scarpa ◽  
T. Tiffert ◽  
F. J. Brinley
Keyword(s):  
Single Cells ◽  
In Vivo Measurements

In Vivo Measurements of Cranial Electrical Stimulation Using Stereotactic-EEG: A Pilot Study

2021 ◽  
Author(s):  
Minmin Wang ◽  
Shenghua Zhu ◽  
Haonan Guan ◽  
Hongjie Jiang ◽  
Jianmin Zhang ◽  
...  
Keyword(s):  
Electrical Stimulation ◽  
Pilot Study ◽  
In Vivo Measurements

scholarly journals Simulating a Ground Truth for Transit Time Analysis of Indicator Dilution Curves

2020 ◽  
Vol 6 (3) ◽  
pp. 268-271
Author(s):  
Michael Reiß ◽  
Ady Naber ◽  
Werner Nahm
Keyword(s):  
Transit Time ◽  
Sampling Rate ◽  
Ground Truth ◽  
Indicator Dilution ◽  
Cross Sectional ◽  
In Vivo Measurements ◽  
Multiple Indicator Dilution ◽  
Transit Times ◽  
Dilution Curves

AbstractTransit times of a bolus through an organ can provide valuable information for researchers, technicians and clinicians. Therefore, an indicator is injected and the temporal propagation is monitored at two distinct locations. The transit time extracted from two indicator dilution curves can be used to calculate for example blood flow and thus provide the surgeon with important diagnostic information. However, the performance of methods to determine the transit time Δt cannot be assessed quantitatively due to the lack of a sufficient and trustworthy ground truth derived from in vivo measurements. Therefore, we propose a method to obtain an in silico generated dataset of differently subsampled indicator dilution curves with a ground truth of the transit time. This method allows variations on shape, sampling rate and noise while being accurate and easily configurable. COMSOL Multiphysics is used to simulate a laminar flow through a pipe containing blood analogue. The indicator is modelled as a rectangular function of concentration in a segment of the pipe. Afterwards, a flow is applied and the rectangular function will be diluted. Shape varying dilution curves are obtained by discrete-time measurement of the average dye concentration over different cross-sectional areas of the pipe. One dataset is obtained by duplicating one curve followed by subsampling, delaying and applying noise. Multiple indicator dilution curves were simulated, which are qualitatively matching in vivo measurements. The curves temporal resolution, delay and noise level can be chosen according to the requirements of the field of research. Various datasets, each containing two corresponding dilution curves with an existing ground truth transit time, are now available. With additional knowledge or assumptions regarding the detection-specific transfer function, realistic signal characteristics can be simulated. The accuracy of methods for the assessment of Δt can now be quantitatively compared and their sensitivity to noise evaluated.

In vivo measurements of glenohumeral distraction technique performed in three different joint positions

2021 ◽  
pp. 1-7
Author(s):  
Diego Guerra-Rodríguez ◽  
Liliana Rozo ◽  
Daniel Basilio ◽  
Juan Guerrero-Henriquez
Keyword(s):  
In Vivo Measurements

In vivo expansion of cells expressing acquired CSF3R mutations in patients with severe congenital neutropenia

Blood ◽  
2009 ◽  
Vol 113 (3) ◽  
pp. 668-670 ◽  
Author(s):  
Manuela Germeshausen ◽  
Karl Welte ◽  
Matthias Ballmaier
Keyword(s):  
Single Cells ◽  
Bone Marrow Failure ◽  
Point Mutations ◽  
Predictive Marker ◽  
Lymphoid Cells ◽  
Lower Percentage ◽  
Severe Congenital Neutropenia ◽  
Congenital Neutropenia ◽  
Bone Marrow Failure Syndrome

Abstract Severe congenital neutropenia (CN) is a rare bone marrow failure syndrome with a high incidence of acute leukemia. In previous studies, we could show that point mutations in the gene for the granulocyte colony-stimulating factor (G-CSF) receptor CSF3R are a highly predictive marker for leukemic development in CN patients. To find out at which stage of hematopoietic development these mutations emerge and how they are propagated during hematopoietic differentiation, we analyzed single cells of different hematopoietic subpopulations from CN patients with CSF3R mutations. We found that CSF3R mutations are not restricted to the myeloid compartment but are also detectable in lymphoid cells, although at a much lower percentage. From our observations, we conclude that CSF3R mutations are acquired in multipotent hematopoietic progenitor cells in CN patients and that they are clonally expanded in myeloid cells expressing the G-CSF receptor due to the growth advantage mediated by the CSF3R mutation.

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