Functional Heterogeneity of Human T Cell Responses to Class I and Class II MHC Antigens

1984 ◽  
pp. 622-623
Author(s):  
N. E. Goeken ◽  
M. K. Eckerle ◽  
P. J. Lioubin ◽  
T. S. Staggs
Keyword(s):  
T Cell ◽  
T Cell Responses ◽  
Class Ii ◽  
Class I ◽  
Functional Heterogeneity ◽  
Mhc Antigens ◽  
Cell Responses ◽  
Human T Cell ◽  
Class Ii Mhc ◽  
Class Ii Mhc Antigens

scholarly journals Identical peptides recognized by MHC class I- and II-restricted T cells.

1989 ◽  
Vol 170 (1) ◽  
pp. 279-289 ◽  
Author(s):  
D L Perkins ◽  
M Z Lai ◽  
J A Smith ◽  
M L Gefter
Keyword(s):  
T Cells ◽  
T Cell ◽  
T Cell Responses ◽  
Class Ii ◽  
Antigen Recognition ◽  
Class I ◽  
Single Class ◽  
Mhc Molecules ◽  
Cell Responses ◽  
Class Ii Mhc

Previous data from many groups show that both class I and class II-restricted T cells recognize short synthetic peptides in the context of their respective MHC molecules (9-18), all of the peptides described to date are restricted to only a single class of MHC molecules; however, structural homology between the class I and II MHC molecules and the use of similar TCRs by class I and II-restricted T cells suggest that antigen recognition mechanisms are similar in both systems. To directly compare antigen recognition in the two systems, we analyzed peptides for the ability to function in both a class I and II-restricted system and found that seven of seven individual peptides tested stimulate both class I and II-restricted T cell responses. In addition, two of the peptides can function in different species stimulating both human class I and murine class II T cell responses. Thus, the process of T cell recognition of antigen in the context of MHC molecules was highly conserved in evolution not only between the class I and class II MHC systems, but also between the murine and human species.

Mouse Schwann cells activate MHC class I and II restricted T-cell responses, but require external peptide processing for MHC class II presentation

2010 ◽  
Vol 37 (2) ◽  
pp. 483-490 ◽  
Author(s):  
Gerd Meyer zu Hörste ◽  
Holger Heidenreich ◽  
Anne K. Mausberg ◽  
Helmar C. Lehmann ◽  
Anneloor L.M.A. ten Asbroek ◽  
...  
Keyword(s):  
T Cell ◽  
Schwann Cells ◽  
Mhc Class I ◽  
Mhc Class Ii ◽  
T Cell Responses ◽  
Class Ii ◽  
Class I ◽  
Peptide Processing ◽  
Cell Responses

scholarly journals Design of Glycopeptides Used to Investigate Class II MHC Binding and T-Cell Responses Associated with Autoimmune Arthritis

PLoS ONE ◽  
2011 ◽  
Vol 6 (3) ◽  
pp. e17881 ◽  
Author(s):  
Ida E. Andersson ◽  
C. David Andersson ◽  
Tsvetelina Batsalova ◽  
Balik Dzhambazov ◽  
Rikard Holmdahl ◽  
...  
Keyword(s):  
T Cell ◽  
T Cell Responses ◽  
Class Ii ◽  
Autoimmune Arthritis ◽  
Cell Responses ◽  
Class Ii Mhc

scholarly journals Alloreactive T Cell Responses and Acute Rejection of Single Class II MHC-Disparate Heart Allografts Are under Strict Regulation by CD4+CD25+ T Cells.

2005 ◽  
Vol 174 (8) ◽  
pp. 5135.2-5135 ◽  
Author(s):  
Soren Schenk ◽  
Danielle D. Kish ◽  
Chunshui He ◽  
Tarek El-Sawy ◽  
Eise Chiffoleau ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
Acute Rejection ◽  
T Cell Responses ◽  
Class Ii ◽  
Single Class ◽  
Cell Responses ◽  
Class Ii Mhc ◽  
Strict Regulation ◽  
Alloreactive T Cell

Enhancement of Class II-Restricted T cell Responses by Costimulatory NK Receptors for Class I MHC Proteins

Science ◽  
1996 ◽  
Vol 274 (5295) ◽  
pp. 2097-2100 ◽  
Author(s):  
O. Mandelboim ◽  
D. M. Davis ◽  
H. T. Reyburn ◽  
M. Vales-Gomez ◽  
E. G. Sheu ◽  
...  
Keyword(s):  
T Cell ◽  
T Cell Responses ◽  
Class Ii ◽  
Class I ◽  
Class I Mhc ◽  
Nk Receptors ◽  
Cell Responses

Expression of class I and class II MHC antigens in neuromuscular diseases

1989 ◽  
Vol 89 (2-3) ◽  
pp. 213-226 ◽  
Author(s):  
Rhoda M. McDouall ◽  
Michael J. Dunn ◽  
Victor Dubowitz
Keyword(s):  
Neuromuscular Diseases ◽  
Class Ii ◽  
Class I ◽  
Mhc Antigens ◽  
Class Ii Mhc ◽  
Class Ii Mhc Antigens

scholarly journals 115 CYTOLOGICAL ANALYSIS OF HEPATIC GENE EXPRESSION AND IMMUNOLOGICAL RESPONSE OF MHC ANTIGENS IN MOUSE AMNIOTIC EPITHELIAL CELLS

2005 ◽  
Vol 17 (2) ◽  
pp. 208
Author(s):  
T. Mitani ◽  
T. Nagai ◽  
D. Suzuki ◽  
Y. Ukida ◽  
H. Kato ◽  
...  
Keyword(s):  
Class Ii ◽  
Class I ◽  
Hepatic Gene Expression ◽  
Rt Pcr ◽  
Mhc Antigens ◽  
Mhc Molecules ◽  
Hepatic Cells ◽  
Class Ii Mhc ◽  
Class Ii Mhc Antigens ◽  
Ifn Γ

Human amniotic epithelial cells (hAECs) have been reported to have unique properties. They express almost no class I and class II MHC antigens and lack response to interferon-γ (IFN-γ) which mediates the expression of those MHC molecules. Moreover, hAECs express some genes characteristic of hepatic cells. Therefore, hAECs seem to have multipotency and are expected to substitute for hepatic tissues in part. We aimed to develop the experimental model for investigating AECs in mice (mAECs). In this study, we examined the induction of MHC molecules by IFN-γ and the hepatic gene expression in mAECs. Murine amniotic membranes were collected from C57BL/6J females at 17.5 days of gestation. They were digested by 0.03% hyaluronidase followed by 0.2% collagenase treatment. Dissociated mAECs were cultured on dishes in DMEM supplemented with 10% FBS at 37°C under 5%CO2 in air. Embryonic fibroblasts (EFs) collected from C57BL/6J fetuses at 13.5 dpc were cultured in the same condition as mAECs. In Experiment I, the effect of IFN-γ on induction of MHC molecules in mAECs was examined. mAECs and EFs cultured in the presence or absence of IFN-γ at 1 × 103 U mL−1 for 72 h were recovered and incubated with FITC-conjugated antibodies against mouse H-2 MHC class I or I-A/I-E MHC class II antigens. The cells were analyzed by flow cytometry. In Experiment II, the expression of the genes in mAECs was examined by RT-PCR. mRNA was purified from adult liver, EFs, fresh mAECs, and mAECs cultured for 5 days. As the genes characteristic for hepatic cells, HNF-3α, HNF-3β, HNF-3γ, HNF-4, transthyretin (TTR), albumin, α-fetoprotein (AFP), glucose-6-phosphatase (G6P), and asialoglycoprotein receptor-1 (Asgr1) were examined. In Experiment I, cell-surface expression of class I and class II MHC antigens in response to IFN-γ was observed weakly in mAECs as compared to EFs, suggesting different property in hAECs which lack the expression of those antigens. In Experiment II, RT-PCR analysis showed that all of the genes except G6P were expressed in fresh mAECs. However, the expression of transcription factors such as HNF-3α, HNF-3β, HNF-4, and TTR, serum proteins such as albumin and AFP, and Asgr1 decreased after in vitro culture, contrary to the case of hAECs in which, for example, albumin appeared after cultivation. In conclusion, it was evident that mAECs have quite different properties, both in the inductivity of MHC molecules and the expression of hepatic genes, from hAECs. This work was supported by Wakayama Prefecture Collaboration of Regional Entities for the Advancement of Technological Excellence of the JST, and by a Grant-in-Aid for the 21st Century COE Program of the Japan MEXT.

Mature dendritic cells pulsed with freeze–thaw cell lysates define an effective in vitro vaccine designed to elicit EBV-specific CD4+ and CD8+ T lymphocyte responses

Blood ◽  
2000 ◽  
Vol 96 (5) ◽  
pp. 1857-1864 ◽  
Author(s):  
Wolfgang Herr ◽  
Elena Ranieri ◽  
Walter Olson ◽  
Hassane Zarour ◽  
Loreto Gesualdo ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
T Cell ◽  
Mhc Class I ◽  
T Cell Responses ◽  
Class Ii ◽  
Interferon Γ ◽  
Class I ◽  
Freeze Thaw ◽  
Cell Responses

Abstract Immunotherapy trials targeting the induction of tumor-reactive T-cell responses in cancer patients appear to hold significant promise. Because nonmutated lineage-specific antigens and mutated idiotypic antigens may be coexpressed by tumor cells, the use of autologous tumor material to promote the broadest range of antitumor T-cell specificities has significant clinical potential in cancer vaccination trials. As a model for vaccination in the cancer setting, we chose to analyze the promotion of T-cell responses against Epstein-Barr virus (EBV)-transformed B-lymphoblastoid cell line (B-LCL)–derived antigens in vitro. A series of bulk antigenic formats (freeze–thaw lysate, trifluoroacetic acid lysate, extracted membranes, affinity-purified MHC class I– and class II–presented peptides, acid-eluted peptides) prepared from EBV B-LCLs were tested for their ability to stimulate EBV B-LCL–reactive CD4+ and CD8+ T lymphocytes in vitro when pulsed onto autologous dendritic cells (DCs). DC presentation of freeze–thaw lysate material derived from (either autologous or allogeneic) EBV B-LCLs with an Mr of 10 kd or larger stimulated optimal anti-EBV B-LCL responsiveness from freshly isolated CD4+ and CD8+ peripheral blood T cells. These in vivo “memory” T-cell responses were observed only in EBV-seropositive donors. CD4+ T-cell responses to lysate-pulsed DCs were Th1 type (ie, strong interferon-γ and weak interleukin-5 responses). While CD8+ T-cell responses were also observed in interferon-γ Elispot assays and in cytotoxicity assays, these responses were of low frequency unless the DC stimulators were induced to “mature” after being fed with tumor lysates. Optimal-length, naturally processed, and MHC class I– or class II–presented tumor peptides were comparatively poorly immunogenic in this model system.

Identification of Novel MHC Class II-Restricted Male-Specific mHAg Encoded bySMCY(JARID1D)..

Blood ◽  
2009 ◽  
Vol 114 (22) ◽  
pp. 1344-1344
Author(s):  
Nobuharu Fujii ◽  
Kellie V Rosinski ◽  
Paulo V Campregher ◽  
Edus H Warren
Keyword(s):  
T Cell ◽  
Mhc Class I ◽  
Mhc Class Ii ◽  
Cell Clone ◽  
T Cell Responses ◽  
Class Ii ◽  
Cd4 T Cell ◽  
Class I ◽  
Cell Responses ◽  
T Cell Clone

Abstract Abstract 1344 Poster Board I-366 Male recipients of female hematopoietic cell grafts, when compared with all other donor/recipient gender combinations, have an increased risk for both acute and chronic GVHD, but also have a significantly decreased risk of posttransplant relapse. F→M HCT is also characterized at the cellular level by donor (female) T cell responses against male-specific minor histocompatibility (H-Y) antigens, which can contribute to both graft-versus-host disease (GVHD) and graft-versus-leukemia (GVL) activity. SMCY is a Y-chromosome gene that has previously been shown to encode at least two distinct MHC class I-restricted H-Y antigens presented by HLA-A*0201 and HLA-B*0702, respectively. Also, association between CD8+ T cell responses specific for the SMCY311-319 FIDSYICQV epitope and GVHD or GVL has been reported. A CD8+ FIDSYICQV-specific T cell clone was also reported to induce histological signs of GVHD reaction in an in vitro skin-explant assay. To date, however, only two MHC class I-restricted, and no MHC class II-restricted, H-Y antigens encoded by SMCY have been characterized. Given the large size of the SMCY and the homologous SMCX proteins and the fact that they are only 85% identical at the amino acid sequence level, we hypothesized that SMCY encodes other MHC class I- and class II-restricted H-Y antigens, and that T cell responses against these epitopes may likewise contribute to GVHD and GVL activity after F→M HCT. Arrays of pentadecapeptides with eleven-residue overlap were designed to tile regions of the SMCY protein that are non-identical to the corresponding regions of its X chromosome-encoded homologue SMCX, and then used to generate SMCY-specific T cell lines recognizing novel SMCY-encoded MHC class I- and class II-restricted H-Y antigens. Peripheral blood mononuclear cells (PBMC) were obtained on posttransplant day +126 from a 46 year-old male patient with monosomy 7 AML who had received a hematopoietic cell graft from his MHC-identical sister, and were stimulated in vitro with dendritic cells derived from his pretransplant PBMC that had been pulsed with the SMCY pentadecapeptides. After three stimulations, a SMCY peptide-specific CD4+ T cell line as well as a SMCY311-319 (FIDSYICQV)-specific CD8+ T cell line were obtained. After cloning by limiting dilution, we further characterized the SMCY-specific CD4+ T cell clone, 13H3. The 13H3 T cell clone recognizes the SMCY232-246 15-mer peptide, ELKKLQIYGPGPKMM, presented by HLA-DRB1*1501, and has a CD3+, CD4+, CD8−, CD45RA−, CD45RO+ surface phenotype. The cytokine release profile of this clone when assessed with SMCY232-246-loaded donor-derived EBV-LCL, as measured by the Luminex assay, is characterized mainly by Th1 cytokines (IFN-g and IL-2), but the clone also produced low to moderate levels of the Th2 cytokines IL-4, IL-10, and TGF-β. A minigene encoding SMCY232-246 was recognized by the 13H3 clone in a HLA-DRB1*1501-dependent fashion when transfected into COS-7 cells, but a minigene encoding the homologous SMCX-derived ELKKLQIYGAGPKMM peptide was not recognized, demonstrating that the clone is SMCY-specific. The 13H3 clone recognized 3 of 5 HLA-DRB1*1501+ male primary leukemia cells, but did not recognize either of 2 HLA-DRB1*1501− male or either of 2 HLA-DRB1*1501+ female primary leukemia cells. These results suggest that CD4+ T cell responses against the SMCY232-246 epitope could potentially contribute to GVL activity after F→M HCT. A SMCY232-246/HLA-DRB1*1501 tetramer has been constructed which specifically marks the 13H3 T cell clone, and future studies will use this reagent to determine whether CD4+ T cells specific for this epitope can be detected directly ex vivo in posttransplant blood samples from HLA-DRB1*1501+ F→M HCT recipients. Disclosures No relevant conflicts of interest to declare.

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