Genetic Variation in in-Vitro Cultures and Regenerated Plants in Tomato and its Implications

1991 ◽  
pp. 259-275 ◽  
Author(s):  
K. Sree Ramulu
Keyword(s):  
Genetic Variation ◽  
In Vitro Cultures ◽  
Regenerated Plants

scholarly journals The effect of media composition on multiplication of grape rootstocks in vitro

2012 ◽  
Vol 60 (8) ◽  
pp. 141-144 ◽  
Author(s):  
Břetislav Křižan ◽  
Eva Ondrušiková ◽  
Jana Moudrá
Keyword(s):  
Mass Production ◽  
Plant Material ◽  
In Vitro Cultures ◽  
Media Composition ◽  
Mass Propagation ◽  
Regenerated Plants ◽  
Optimized Protocol ◽  
Embryogenic Calluses ◽  
Murashige Skoog

The current demand for in vitro cultures of grape rootstocks, not only for mass production of plants, but also for genetic engineering is evident. The study on micropropagation of grape rootstock genotypes namely Kober 5BB, Kober 125AA and Teleki 5C was performed. The aim of the study was to develop an optimized protocol to obtain large quantity of plant material. Protocol is based on regeneration via organogenesis, considering that grape embryogenic calluses are laborious to establish and the genotype of the regenerated plants can be altered. Using of Driver and Kuniyuki Walnut media for the establishing of proliferating cultures gave better results than Murashige Skoog media in case of all used rootstocks. Subsequent cultivation on modified Murashige Skoog media with 1-naphtalene acetic acid and increased concentration of cytokynin was characterized by multiplication of cultures and formation of clusters with high multiplication capability. The clusters obtained from rootstock genotypes were suitable for mass propagation as well as for genetic transformation due to their high ability of regeneration.

scholarly journals Micropropagation and assessment of somaclonal variation in Galanthus transcaucasicus in vitro plantlets

2021 ◽  
Vol 27 (4) ◽  
pp. 505-515
Author(s):  
Narges Asadi ◽  
Hossein Zarei ◽  
Seyyed Hamidreza Hashemi-Petroudi ◽  
Seyyed Javad Mousavizadeh
Keyword(s):  
Genetic Variation ◽  
Plant Growth ◽  
In Vitro Culture ◽  
Somaclonal Variation ◽  
Growth Regulators ◽  
Naphthaleneacetic Acid ◽  
In Vitro Plantlets ◽  
Regenerated Plants ◽  
High Concentrations

Abstract In vitro culture of twin-scaling explants of Galanthus transcaucasicus with different concentrations of plant growth regulators (PGRs) including 0.5, 1, 2, 3, 4, 6, 8, and 10 mg L-1 naphthaleneacetic acid (NAA) and 0.5, 1, 2, 3, and 4 mg L-1 benzyladenine (BA) was studied. After 18 weeks, the number of regenerated bulblets and intensity of callus was measured. Subsequently, bulblets were transferred to a medium with 0.5, 1, 2, 3, and 4 mg L-1 NAA and 0.5, 1, 2, 3, and 4 mg L-1 BA and, after 15 weeks, the bulblets length and diameter were measured. The highest intensity of callus was obtained on 4 mg L-1 NAA or 8 mg L-1 NAA with 1 mg L-1 BA. The highest number of regenerated bulblets was detected with 6 mg L-1 NAA and 2 mg L-1 BA. The highest diameter of bulblets occurred on four mgL-1 NAA (9.4 mm), while the lowest was observed on 0.5 mg L-1 BA (1.83 mm). The analysis of genetic variation using ISSR revealed that there was no somaclonal variation among the regenerated plants from BA and low level of NAA, but there was a significant somaclonal variation at high concentrations of NAA.

scholarly journals Somaclonal Variation in Habanero Pepper (Capsicum chinense Jacq.) as Assessed ISSR Molecular Markers

HortScience ◽  
2014 ◽  
Vol 49 (4) ◽  
pp. 481-485 ◽  
Author(s):  
Jericó J. Bello-Bello ◽  
Lourdes G. Iglesias-Andreu ◽  
Susana A. Avilés-Viñas ◽  
Eunice Gómez-Uc ◽  
Adriana Canto-Flick ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Issr Markers ◽  
Direct Organogenesis ◽  
Capsicum Chinense ◽  
Distance Analysis ◽  
System A ◽  
Regenerated Plants ◽  
Habanero Pepper ◽  
Genetic Distance Analysis

Intersimple sequence repeat (ISSR) markers were used to evaluate the effects of in vitro culture on genetic variation in Habanero pepper (Capsicum chinense Jacq.) regeneration protocols. A total of 219 ISSR clear and reproducible fragments were generated with 13 ISSR primers in direct organogenesis, direct and indirect somatic embryos, and the embryogenic callus system. A cluster analysis was performed to express in the form of dendrogram the relationships among different regeneration systems and the genetic variability detected. Genetic distance analysis indicated that our regeneration protocols are inappropriate for micropropagation, conservation, or genetic transformation; however, they may be applicable to breeding. This is the first report on the use of molecular analysis to evaluate genetic variation of in vitro-regenerated plants of Habanero pepper using ISSR markers.

scholarly journals In vitro cultures of Blackstonia perfoliata (L.) Hudson and an assay of the secoiridoid content

2014 ◽  
Vol 61 (3-4) ◽  
pp. 359-368 ◽  
Author(s):  
Lutosława Skrzypczak ◽  
Maria Wesołowska ◽  
Anna Krupińska ◽  
Barbara Thiem
Keyword(s):  
Medicinal Plant ◽  
Callus Tissue ◽  
In Vitro Cultures ◽  
Regenerated Plants

<i>In vitro</i> cultures of <i>Blackstonia perfoliata</i> (L.) Hudson, a popular medicinal plant, were initiated. Regenerated plants and callus tissue were obtained and then analysed for secoiridoids.

scholarly journals Genetic diversity and volatile oil components variation in Achillea fragrantissima wild accessions and their regenerated genotypes

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Abdelfattah Badr ◽  
Hanaa H. El-Shazly ◽  
Mahmoud Sakr ◽  
Mai M. Farid ◽  
Marwa Hamouda ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Essential Oil ◽  
Volatile Oil ◽  
Habitat Destruction ◽  
Regenerated Plants ◽  
Achillea Fragrantissima ◽  
Oil Components ◽  
Wild Accessions

Abstract Background Wild medicinal plants are suffering natural environmental stresses and habitat destruction. The genetic diversity evaluation of wild accessions and their in vitro raised genotypes using molecular markers, as well as the estimation of substances of pharmaceutical value in wild plants and their regenerated genotypes are convenient approaches to test the genetic fidelity of regenerated plants as a source of substances of pharmaceutical value. In this study, the genetic diversity of 12 accessions of the medicinal plant Achillea fragrantissima, representing five sites in the mountains of South Sinai, Egypt, were estimated by the inter simple sequence repeats (ISSR) fingerprinting and their volatile oil components were identified using gas chromatography-mass spectrometry (GC-MS) analysis. The same accessions were regenerated in vitro and the genetic diversity and volatile oil components of propagated genotypes were determined and compared to their wild parents. Results Clustering and principal component analyses indicated that the wild accessions and their regenerated genotypes were genetically differentiated, but the regenerated plants are relatively more diverse compared to their wild parents. However, genetic variation between wild accessions is inherited to their in vitro propagated genotypes indicating genotypic differentiation of the examined accessions. The number of volatile oil compounds in the wild A. fragrantissima accessions was 31 compounds while in the in vitro propagated plants only 24 compounds were detected. Four major compounds are common to both wild and regenerated plants; these are artemisia ketone, alpha-thujone, dodecane, and piperitone. Conclusions Genome profiling and essential oil components analysis showed variations in A. fragrantissima accessions from different populations. Genetic differences between wild and regenerated genotypes were analyzed and validated with the final conclusion that in vitro conditions elicited higher genetic variation that is associated with reduced amount and diversity in the essential oil components.

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